Microproteomics Analysis Service
Microproteomics Analysis Service refers to a specialized analytical service designed for protein samples in the nanogram to microgram range. It employs low-loss sample preparation methods combined with high-sensitivity mass spectrometry platforms to enable comprehensive protein identification, quantification, and functional annotation.
In traditional proteomics research, hundreds of micrograms to milligram-level protein inputs are typically required. However, in fields such as clinical research, histopathology, single-cell biology, and pharmacological studies, researchers often encounter challenges involving scarce or non-replicable samples. Examples include laser-captured tissue sections, biopsy specimens, subcellular fractions obtained via ultracentrifugation, and trace biological fluids. To address the omics analysis needs of these high-value and limited samples, Microproteomics has emerged as a vital solution.
Pellegrini D. et al. Molecular&Cellular Proteomics. 2019.
Microproteomics leverages a suite of optimized strategies tailored for low-input samples, such as efficient protein extraction, simplified digestion workflows, and SP3 bead-based peptide enrichment, to minimize sample loss and enhance signal-to-noise ratios. This streamlined process eliminates traditional steps like protein quantification, gel electrophoresis, and multiple transfer operations, thereby improving recovery rates and ensuring processing consistency. Combined with high-sensitivity mass spectrometry platforms and dedicated low-input preparation protocols, this approach has become a key enabler of high-precision, low-abundance proteomic research.
With cutting-edge chromatography and mass spectrometry platforms, MtoZ Biolabs offers Microproteomics Analysis Service capable of achieving quantitative protein identification and deep proteome coverage from as little as 1μg of protein or approximately 100 cells. This service is broadly applicable to biomarker discovery, drug mechanism elucidation, and spatial proteomics in a wide range of biological and clinical research scenarios.
Analysis Workflow
The main workflow of Microproteomics Analysis Service includes:
1. Sample Reception and Pre-Evaluation
Micro-scale biological samples of various types are received and undergo condition-based pre-assessment.
2. Protein Extraction and Digestion
Proteins are extracted and digested using a one-step protocol with trypsin to maximize peptide yield from low-input material.
3. Peptide Purification and Enrichment
SP3 magnetic bead-based technology is employed to efficiently enrich and purify trace peptides while removing contaminants.
4. Mass Spectrometry Analysis
Protein identification and quantification are performed using an Astral high-resolution mass spectrometer coupled with a nano-LC system.
5. Data Processing and Annotation
Dedicated software is used to carry out quantitative analysis, differential expression profiling, functional enrichment, and pathway annotation.
Sample Submission Suggestions
Sample Types: Supports a wide range of low-input sample types, including microdissected tissues, biopsy samples, cultured cells, cerebrospinal fluid, and more.
Storage Conditions: All samples should be snap-frozen and stored at -80°C. During transport, use dry ice to maintain ultra-low temperatures. For cellular samples, lysis buffer may be added prior to freezing to stabilize protein content.
Additional Notes: Please provide as much sample background information as possible (e.g., tissue origin, processing method, experimental grouping, and research objectives) to facilitate optimized experimental workflows and tailored data analysis strategies.
Service Advantages
1. Low Sample Input: Requires as little as 1μg of protein or approximately 100 cells for detection.
2. One-Step Protein and Peptide Extraction: Deeply optimized sample preparation enables simultaneous extraction of proteins and peptides, achieving recovery rates of up to 90%.
3. Advanced Analysis Platform: MtoZ Biolabs established an advanced Microproteomics Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
4. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
5. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Microproteomics Analysis data, providing clients with a comprehensive data report.
Applications
Example application scenarios of Microproteomics Analysis Service：
Trace Biomarker Discovery
Identify disease-associated proteins from limited sample quantities for early prediction, classification, and prognosis evaluation.
Drug Target and Mechanism of Action Studies
Analyze proteomic changes in target cells or tissues before and after drug treatment to uncover signaling pathways and potential molecular targets.
Support for Spatial and Single-Cell Proteomics
Combined with tissue imaging and single-cell isolation techniques, this approach helps elucidate protein function and interactions within the tissue microenvironment.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Mass Spectrometry Based Proteomics Analysis Service
How to order?
