Methylated Peptide Analysis Service
- Raw data files from LC-MS/MS platforms
- Processed data tables in Excel or CSV format containing identified and quantified methylated peptides
- Summary reports in PDF format including analytical methods, quality control results, and key findings
- High-resolution spectra and charts in TIFF or PNG formats
- Additional data formats can be provided upon request to meet publication or specific project needs.
- Cell lysates or nuclear extracts for intracellular methylation studies
- Tissue homogenates for in vivo modification analysis
- Recombinant or fusion proteins for targeted research
- Synthetic peptides for method validation
MtoZ Biolabs provides methylated peptide analysis service using high-resolution mass spectrometry platforms and specific enrichment strategies to help researchers systematically identify and quantify methylation modification sites in proteins, thereby revealing their biological roles in epigenetic regulation, signal transduction, and disease development. This service is applicable to cell, tissue, and recombinant protein samples, providing reliable data and technical support for post-translational modification studies.
What is Protein Methylation
Protein methylation is a common form of post-translational modification that mainly occurs on lysine (Lys) or arginine (Arg) residues. By adding methyl groups to these amino acids, methylation can alter the charge distribution, structural conformation, and intermolecular interactions of proteins.
Biggar, KK. et al. Nat Rev Mol Cell Biol. 2015.
Figure 1. Lys and Arg Methylation
This modification plays a key role in chromatin remodeling, gene expression regulation, signal transduction, and cell cycle control. Abnormal methylation is associated with various diseases, including cancer, metabolic disorders, and neurodegenerative diseases. Therefore, the study of methylated peptides is of great importance for both basic biological research and drug development.
Methylated Peptide Analysis Service at MtoZ Biolabs
The methylated peptide analysis service at MtoZ Biolabs combines highly sensitive mass spectrometry detection with enrichment strategies to comprehensively analyze methylation modifications in complex samples. Main services include:
1. Methylated Peptide Identification: High-resolution LC-MS/MS systems such as Orbitrap Fusion Lumos and Q Exactive HF are used with database searching to accurately identify methylation sites.
2. Quantitative Analysis: Supports multiple quantification strategies such as Label-free, TMT, and iTRAQ to compare methylation levels under different conditions.
3. Multi-Site Modification Analysis: Distinguishes between mono-, di-, and tri-methylation forms.
4. Functional Correlation Analysis: Uses bioinformatics tools to explore the relationships between methylation, signaling pathways, protein functions, and disease mechanisms.
MtoZ Biolabs can design customized experimental workflows based on specific research goals and sample types.
Workflow of Methylated Peptide Analysis Service
Why Choose MtoZ Biolabs
☑️ Advanced mass spectrometry platforms such as Orbitrap Fusion Lumos and Q Exactive HF.
☑️ Experienced scientific team with strong expertise in post-translational modification studies.
☑️ High-sensitivity enrichment for low-abundance methylated peptides.
☑️ Flexible and customizable analysis strategies based on research objectives.
☑️ Reliable and reproducible data suitable for publication.
Applications of Methylated Peptide Analysis Service
1️⃣ Studying chromatin remodeling and epigenetic regulation mechanisms.
2️⃣ Analyzing methylation-dependent signaling networks.
3️⃣ Identifying methylation-related disease targets.
4️⃣ Supporting drug target screening and mechanism validation.
5️⃣ Integrating multi-omics data to reveal cellular dynamics.
Sample Submission Guidelines
For special sample types, please contact the MtoZ Biolabs technical team for personalized guidance.
Deliverables
1. Experimental workflow and instrument parameters
2. Raw mass spectrometry data files
3. Methylation site identification tables
4. Quantitative analysis reports and statistical charts
5. Data quality control and evaluation reports
FAQs
Q1: What is the service's general workflow?
Q2: What data formats are provided?
MtoZ Biolabs provides multiple standardized data formats to ensure compatibility with various analytical and visualization tools. Typical deliverables include:
Q3: How should I prepare my samples?
To ensure accurate and reproducible methylated peptide analysis, MtoZ Biolabs recommends the following preparation guidelines:
1. Sample Type: Cell lysates, tissue samples, serum, recombinant proteins, and synthetic peptides are accepted.
2. Sample Purity: Samples should be free from particulates, high salts, detergents, or organic solvents.
3. Sample Volume: Provide at least 500 µL of liquid sample or 100 mg of solid material. Larger volumes are recommended for replicate analyses.
4. Storage and Shipping: Store samples at −80°C and ship on dry ice to prevent degradation.
5. Documentation: Include details such as sample origin, extraction method, buffer composition, and analysis objectives.
For more information, please refer to Sample Submission Guidelines for Proteomics and Sample Submission Guidelines for Metabolomics.
If you are unsure about your sample preparation, our technical team can provide personalized pre-analysis consultation and a sample submission guide to help you optimize preparation and shipping conditions.
Q4: What types of samples are suitable?
This service is suitable for a variety of biological samples, including:
MtoZ Biolabs can recommend the most appropriate enrichment and analysis strategy based on the sample type and research goals.
Start Your Project with MtoZ Biolabs
Protein methylation plays a vital role in regulating gene expression and cellular function. With advanced mass spectrometry platforms and optimized analytical workflows, MtoZ Biolabs provides a comprehensive methylated peptide analysis service to help researchers uncover the molecular mechanisms of protein modification networks and disease processes. Contact us to learn more or request a customized project quote.
How to order?
