Isomaltooligosaccharide Analysis Service

MtoZ Biolabs utilizes high-resolution HPLC, LC-MS, and NMR analytical platforms to provide the isomaltooligosaccharide analysis service which enables precise analysis of the composition, structural characteristics, and content of isomaltooligosaccharides. This service is widely applied in studies of polysaccharide hydrolysis products, functional oligosaccharide development, and carbohydrate metabolism research, offering reliable data support for glycan structure elucidation and biological function analysis.

 

What Is Isomaltooligosaccharide?

Isomaltooligosaccharides (IMOs) are a type of oligosaccharide composed of glucose units primarily linked by α-1,6 glycosidic bonds, typically produced through enzymatic transglycosylation of starch. Due to their high resistance to digestion and prebiotic effects, IMOs can promote the proliferation of beneficial bacteria and improve intestinal health. They have extensive applications in fermentation engineering, metabolic regulation, and functional oligosaccharide research, serving as an important subject for exploring the relationship between carbohydrate structures and physiological functions.

 



Sorndech, W. et al. LWT, 2018.

Figure 1. IMOs Common Names and Chemical Structures.

 

Isomaltooligosaccharide Analysis Service at MtoZ Biolabs

1. Quantitative Analysis

Utilizing high-sensitivity chromatographic-mass spectrometric platforms to accurately quantify isomaltooligosaccharides, ensuring reliable and reproducible results.

 

2. Purity Analysis

Evaluating the purity and impurity content of target components in the sample to provide reference data for subsequent studies.

 

3. Structural Characterization

Combining mass spectrometry and nuclear magnetic resonance (NMR) techniques to elucidate glycosidic bond types and molecular configurations, revealing the structural features of isomaltooligosaccharides.

 

4. Degree of Polymerization Distribution

Using high-performance gel filtration chromatography (HPGFC) to determine the proportion of different polymerization degrees and assess molecular weight distribution.

 

5. Stability Evaluation

Assessing the structural stability and degradation characteristics of isomaltooligosaccharides under various conditions.

 

6. Bioactivity Analysis

Investigating the prebiotic functions and biological activities of isomaltooligosaccharides within biological systems.

 



Villéger, R. et al. Applied Sciences, 2022.

Figure 2. Structure of Commercial IMOs determined by MS.

 

Why Choose MtoZ Biolabs?

✅ High-Sensitivity Detection: Enables precise quantification and structural elucidation of isomaltooligosaccharides.

✅ Multidimensional Analysis: Covers multiple analytical dimensions, including composition, purity, and degree of polymerization.

✅ Customized Solutions: Experimental designs are flexibly adjusted based on sample characteristics and research objectives.

✅ One-Time Pricing: Transparent pricing with no hidden fees or additional costs.

✅ One-Stop Service: Professional support throughout the entire process, from sample preparation to report delivery.

 

Applications of Isomaltooligosaccharide Analysis Service

1. Enzymatic Reaction Studies

Used to analyze the catalytic efficiency and reaction pathways of glycosidases or transferases acting on isomaltooligosaccharides.

 

2. Metabolic Mechanism Analysis

Reveals the key roles of isomaltooligosaccharides in cellular energy metabolism and polysaccharide degradation processes.

 

3. Fermentation Process Evaluation

Monitors microbial utilization efficiency and changes in product composition during fermentation involving isomaltooligosaccharides.

 

4. Functional Oligosaccharide Research

Provides data support for structural optimization and bioactivity validation of novel oligosaccharides.

 

5. Biotechnological Applications

Facilitates studies on polysaccharide biosynthesis pathway modification and carbohydrate metabolism regulation.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Quantitative Data of Isomaltooligosaccharides

4. Structural Characterization Results

5. Degree of Polymerization Distribution Profiles

6. Raw Mass Spectrometry and Chromatography Data Files

7. Bioinformatics Analysis

 

FAQ

Q1: What types of samples are suitable?

A1: The isomaltooligosaccharide analysis service provided by MtoZ Biolabs is suitable for various carbohydrate samples, including purified isomaltooligosaccharides, enzymatic hydrolysates, fermentation broths, plant extracts, and related biological materials. To ensure stable and reliable analytical results, samples should be free of visible impurities, suspended particles, or protein residues and should undergo appropriate desalting or filtration to minimize interference.

 

Q2: What is the service general workflow?

A2:



 

Q3: What data formats are provided?

A3: MtoZ Biolabs delivers standardized data in multiple formats to ensure compatibility with research analysis and academic publication. Typical deliverables include:

• Raw data files generated from LC-MS, HPLC, or NMR platforms

• Processed result tables (Excel or CSV format) containing retention times, molecular weights, degrees of polymerization, and relative abundance information

• Analytical reports (PDF format) detailing experimental workflow, data quality evaluation, and key findings

• Chromatographic and mass spectrometric images (TIFF or PNG format) for visualization and comparison

Customized formats can be provided upon request for specific research needs.

 

Q4: How should I prepare my samples?

A4: To ensure accuracy and reproducibility in isomaltooligosaccharide analysis, MtoZ Biolabs recommends the following sample preparation guidelines:

(1) Sample type: Both liquid and lyophilized powder forms are acceptable.

(2) Sample purity: Avoid high salt concentrations, lipids, surfactants, or other interfering substances.

(3) Storage conditions: Store at -20°C for short-term and -80°C for long-term preservation.

(4) Shipping requirements: Ship samples on dry ice using sealed, leak-proof containers.

(5) Additional information: Provide detailed information about sample source, pretreatment procedures, and analysis objectives to ensure the most appropriate analytical design.

For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.

 

Start Your Project with MtoZ Biolabs

Contact us to discuss your experimental design or request a quote. Whether you are exploring its structural characteristics or investigating its roles in metabolic and biological systems, MtoZ Biolabs provides you with high-precision analytical support.