Intact Glycoproteins Analysis Service
- Biomarker Screening
- Biopharmaceutical Quality Control
- Structure-Function Relationship Studies
- Drug Development and Process Optimization
Intact Glycoproteins Analysis Service directly analyzes the molecular weight, glycosylation patterns, and heterogeneity of glycoproteins without enzymatic hydrolysis. This service preserves the native conformation and complete modification information of glycoproteins, and can simultaneously obtain comprehensive information about the protein backbone and the sugar chains it carries in a single analysis, providing a global perspective for structural characterization, quality consistency evaluation, and biological function research.
Glycoproteins play essential roles in cellular communication, signal transduction, immune regulation, protein stability, and pharmacokinetics. Variations in glycosylation type, site, and structural features can directly impact their biological functions and clinical performance. In biopharmaceutical and life science research, particularly in the development and quality control of monoclonal antibodies, recombinant proteins, and glycosylated vaccines, precise characterization of the entire glycoprotein is crucial. While traditional glycan release and peptide mapping methods provide valuable localized information, they may overlook the overall modification landscape. Intact glycoprotein analysis, by leveraging advanced high resolution mass spectrometry and optimized separation strategies, captures the complete glycosylation profile at the intact molecule level without disrupting the tertiary structure. This makes it a powerful tool for biopharmaceutical quality control, batch to batch consistency evaluation, and advanced structural biology research.
Domínguez-Vega E. et al. Talanta. 2018.
Figure 1. HILIC-MS of Intact Erythropoietin.
Services at MtoZ Biolabs
Leveraging advanced mass spectrometry and chromatography platforms, MtoZ Biolabs offers Intact Glycoproteins Analysis Service that enables comprehensive characterization of key features such as protein molecular weight, glycosylation types, heterogeneity distribution, and relative abundance, while preserving the native conformation of glycoproteins. This service delivers high sensitivity and high accuracy data to support biopharmaceutical development, quality consistency evaluation, and structure-function studies.
Analysis Workflow
The main workflow of the Intact Glycoproteins Analysis Service is as follows:
1. Sample Preparation
Remove interfering substances such as salts and surfactants, and perform buffer exchange if necessary to maintain the integrity and stability of glycoproteins.
2. Separation and Purification
Use liquid chromatography techniques such as RP-LC, HILIC, or SEC to separate the target glycoproteins and reduce impurity interference.
3. Mass Spectrometry Analysis
Apply high-resolution mass spectrometry to obtain the intact molecular mass, combined with HCD or ETD to accurately identify glycosylation sites and their combinations.
4. Data Analysis
Use specialized software to analyze molecular weight, glycan composition, modification distribution, and heterogeneity.
Senini I. et al. Commun Chem. 2024.
Figure 2. Workflow for Direct Glycosylation Analysis from Intact Standard and Glycoengineered mAbs by ESI MS and MALDI-ISD MS.
Service Advantages
Advanced Analysis Platform: MtoZ Biolabs established an advanced Intact Glycoproteins Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Intact Glycoproteins Analysis Service data, providing clients with a comprehensive data report.
Preservation of Molecular Integrity: Directly analyze glycoproteins under native conditions to avoid the loss of structural information caused by enzymatic digestion.
Comprehensive Heterogeneity Profiling: Simultaneously characterize different glycosylation forms, subtle mass differences, and structural variations.
Sample Submission Suggestions
Sample Types
MtoZ Biolabs accepts a wide range of biological samples, including purified glycoproteins, protein drug formulations, cell culture supernatants, and serum/plasma.
Storage and Transportation
Store at low temperatures for short-term preservation, and freeze at −80°C for long-term storage. Ship on dry ice and avoid repeated freeze-thaw cycles.
It is recommended to contact the MtoZ Biolabs technical team prior to sample submission to obtain detailed and tailored guidelines for sample preparation and submission.
Applications
Examples of applications for Intact Glycoproteins Analysis Service:
Monitor disease-associated glycan profile changes to provide insights for diagnosis and prognosis.
Perform comprehensive assessment of glycosylation patterns, occupancy, and batch-to-batch variations of therapeutic glycoproteins to ensure production stability and consistent efficacy.
Analyze the impact of glycosylation on protein conformation, stability, receptor binding activity, and immunogenicity to elucidate its role in regulating biological functions.
Provide essential analytical data for glycoengineering, candidate drug screening, and optimization of glycoprotein production processes.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
Related Services
Glycan Composition Analysis Service
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