Infrared Spectrometry Based Polysaccharide Analysis Service

MtoZ Biolabs has launched the infrared spectroscopy based polysaccharide analysis service which enables systematic analysis of the chemical structure, functional group composition, and intermolecular interactions of polysaccharides. This service is widely applied in natural product research, biomaterial development, and polysaccharide quality control, providing reliable data support for polysaccharide structure identification and functional studies.

 

Principle of Infrared Spectrometry

Infrared spectroscopy (IR) is a technique used to analyze chemical structures by detecting the absorption characteristics of molecules toward infrared radiation. Different chemical bonds generate characteristic absorption peaks within specific wavenumber ranges, which can be used to identify the composition and functional groups of substances. In polysaccharide analysis, infrared spectroscopy can detect structural features such as hydroxyl, carboxyl, and glycosidic bonds, determine ring conformations and substitution variations, and, with the aid of fourier transform infrared spectroscopy (FTIR), achieve high-resolution detection, providing essential support for polysaccharide structure elucidation and property studies.

 



Faramarzi, B. et al. Biophysica, 2023.

Figure 1. Schematic Representation of  FT-IR Spectrometer.

 

Infrared Spectrometry Based Polysaccharide Analysis Service at MtoZ Biolabs

MtoZ Biolabs utilizes advanced infrared spectroscopy (IR) technology to provide clients with precise detection services for polysaccharide structures and compositions, enabling the analysis of key information such as chemical bond types, ring conformations, and substitution characteristics.

• Identify major functional groups in polysaccharides (such as hydroxyl, carboxyl, and ether bonds);

• Determine glycosidic bond types and branching structural features;

• Analyze polysaccharide crystallinity, intermolecular interactions, and conformational changes;

• Compare structural differences of polysaccharides from different sources or under varying treatment conditions.

Our analysis offers a comprehensive understanding of the chemical structures and functional properties of polysaccharides, helping researchers gain deeper insights into their structural foundations and biological functions.

 



Roberto, A D. et al. Marine Biotechnology, 2019.

Figure 2. FTIR Spectroscopy of the Polysaccharides Obtained from L. ochroleuca, P. umbilicalis, and G. corneum.

 

Workflow of Infrared Spectrometry Based Polysaccharide Analysis Service

1. Sample Preparation

The provided polysaccharide samples are purified, properly dried, and homogenized to ensure sample uniformity and representativeness.

 

2. Spectral Acquisition

Infrared spectroscopy is used for detection to obtain the characteristic absorption spectra of the samples.

 

3. Data Processing

Baseline correction, smoothing, and normalization are performed to remove background interference and obtain high-quality spectra.

 

4. Characteristic Peak Analysis

The characteristic absorption peaks of polysaccharides are analyzed to identify major functional groups and glycosidic bond types, inferring molecular structural features.

 

5. Result Reporting

A comprehensive report containing spectral data and structural interpretation is provided, offering reliable reference information for polysaccharide characterization studies.

 

Why Choose MtoZ Biolabs?

✅ High-Sensitivity Detection: Rapidly identifies subtle structural differences in polysaccharide samples.

✅ Non-Destructive Analysis: Obtains complete spectral information without damaging the sample.

✅ Comprehensive Structural Interpretation: Reveals glycosidic bond types, branching structures, and functional group characteristics of polysaccharides.

✅ Professional Technical Support: Provided by an experienced spectroscopy and glycoanalysis team offering customized solutions.

✅ One-Stop Service: Covers the entire process from sample testing to data interpretation and report generation.

 

Applications of Infrared Spectrometry Based Polysaccharide Analysis Service

1. Natural Product Research

Used to analyze the chemical structures and functional group characteristics of polysaccharides derived from plants, microorganisms, and marine sources.

 

2. Process Optimization and Monitoring

Applied to monitor structural changes of polysaccharides during fermentation or extraction processes, providing data support for process improvement.

 

3. Metabolism and Stability Studies

Monitors structural variations of polysaccharides under different environmental or treatment conditions, revealing their stability and degradation properties.

 

4. Quality Control and Standardization

Used for purity assessment and batch consistency evaluation of polysaccharide products, ensuring reliability in production and application.

 

5. Structure-Activity Relationship Studies

Elucidates the relationship between polysaccharide structural features and their physicochemical properties or biological activities, supporting functional research.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Polysaccharide Structural Analysis Results

4. Infrared Spectrometry Data and Spectra

5. Comprehensive Analysis Report

6. Raw Data Files

 

FAQ

Q1: What types of samples are suitable?

A1: The infrared spectrometry based polysaccharide analysis service provided by MtoZ Biolabs is suitable for polysaccharide samples from various sources, including natural polysaccharides, microbial polysaccharides, and their derivatives. To ensure clear spectra and reliable results, samples should have high purity, be free from proteins, salts, and other interfering impurities, and remain well-dried and homogeneous.

 

Q2: What is the service general workflow?

A2:



 

Q3: What data formats are provided?

A3: The service deliverables include standardized, multi-format data that facilitate scientific analysis and further applications. Regular deliverables include:

• Raw spectral data files;

• Spectral image files (TIFF or PNG format);

• Tables of characteristic peaks and structural interpretation (Excel format);

• A comprehensive analysis report (PDF format) including experimental conditions and structural explanations.

If special analytical requirements exist, data formats can be customized according to project specifications.

 

Q4: How should I prepare my samples?

A4: To ensure analytical accuracy and reproducibility, it is recommended that sample preparation follow these guidelines:

(1) Sample form: lyophilized powder or solution forms are acceptable;

(2) Purity: avoid samples containing high salt concentrations or surfactants;

(3) Storage conditions: store at -20℃ for short-term and -80℃ for long-term preservation;

(4) Shipping: transport with cold chain or dry ice, ensuring sealed, leak-proof containers clearly labeled with sample information;

(5) Information provided: specify sample source and treatment method to optimize analytical parameters.

For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.

 

Start Your Project with MtoZ Biolabs    

Contact us to discuss your experimental design or request a quote. Whether you are exploring polysaccharide structural characteristics and functional group distribution or studying their metabolism and stability, MtoZ Biolabs provides professional and precise infrared spectroscopic analysis support.