Human Milk Oligosaccharide Microarray Service
- Human Milk Nutrition Research
- Infant Immunity and Microbiota Development Analysis
- Disease-Related Mechanism Studies
- Nutritional Product Development
Human Milk Oligosaccharide Microarray Service is a specialized analytical service based on high-density microarray technology. This service involves immobilizing a variety of structurally defined human milk oligosaccharides (HMOs) onto the surface of a microarray to systematically analyze their binding specificity and functional profiles with biological molecules such as bacteria, viruses, and receptor proteins. It enables high-throughput and quantitative assessment of the roles HMOs play in anti-infection, host defense, gut homeostasis, and immune regulation. This service provides precise support for research in nutritional science, microbiome studies, biopharmaceutical development, and personalized medicine.
Human milk oligosaccharides are the third most abundant solid component in breast milk, after lactose and lipids. They possess remarkable structural diversity and biological activity, making them highly valuable for research in neonatal health, immunology, and microbiology. Understanding the interactions between HMOs and pathogens, immune-related molecules, or host receptors is critical for uncovering the functions of breast milk in early infant development and for advancing the development of infant nutritional supplements, prebiotics, and vaccine adjuvants. The human milk oligosaccharide microarray is a high-throughput platform that enables systematic analysis of interactions between HMOs and biological molecules such as bacteria, immune components, and receptor proteins. This technology involves immobilizing multiple structurally defined HMOs onto a solid-phase microarray and incubating them with labeled target molecules such as immune factors or pathogen-associated glycan-binding proteins. It helps clarify the mechanisms by which HMOs contribute to immune modulation and infection prevention.
MtoZ Biolabs provides Human Milk Oligosaccharide Microarray Service to systematically evaluate the specific binding interactions between HMOs and biological targets including pathogens, immune receptors, and gut microbiota-related molecules. This service can be used to study the mechanisms through which HMOs regulate immune responses, combat infections, support gut development, and contribute to nutritional functions. It is widely applicable in research areas such as infant nutrition, microbiome-based interventions, functional food development, and basic medical research.
Singh RP. et al. Food Res Int. 2022.
Figure 1. Schematic overview of imperative roles of HMOs on the health of infants.
Analysis Workflow
The main workflow of the Human Milk Oligosaccharide Microarray Service is as follows:
1. Oligosaccharide Selection and Microarray Construction
A variety of structurally defined HMOs are selected and immobilized onto the microarray chip surface through chemical conjugation or related methods to construct a high-density and structurally diverse glycan array.
2. Sample Preparation and Labeling
Target molecule samples are prepared according to the research objective and labeled using fluorescent or enzyme-based markers.
3. Incubation and Binding Reaction
Labeled samples are incubated with the HMO microarray, followed by rigorous washing steps to remove non-specific interactions.
4. Signal Acquisition and Image Scanning
A high-resolution fluorescence scanner is used to capture binding signal images and record fluorescence intensities at each glycan spot.
5. Data Analysis and Report Generation
Bioinformatics tools are applied to standardize the raw data, perform statistical analysis, and visualize results. Binding profiles, heatmaps, and functional annotations are generated and compiled into a comprehensive analysis report.
Service Advantages
Advanced Platform: MtoZ Biolabs established an advanced Human Milk Oligosaccharide Microarray Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Milk Oligosaccharide Microarray Service data, providing clients with a comprehensive data report.
Customizable Service Support: Personalized services are available, including custom array design and data analysis, to meet the specific needs of different research projects.
Sample Submission Suggestions
This service supports a variety of sample types. To ensure optimal experimental performance, it is recommended to consult with the MtoZ Biolabs technical team prior to sample submission to discuss project background and sample details. A detailed and tailored sample preparation and shipping guide will be provided.
Applications
Application examples of the Human Milk Oligosaccharide Microarray Service:
Systematically compare HMO profiles across different populations and lactation stages to evaluate their differences in nutritional support and underlying regulatory mechanisms for infants.
Explore the functional roles of HMOs in shaping the infant gut microbiome and promoting the maturation of the immune system.
Screen HMO binding characteristics associated with gastrointestinal disorders, immune dysregulation, or infection susceptibility to support biomarker discovery and mechanistic research.
Facilitate the screening and validation of key oligosaccharide components in infant formula and functional supplements to enhance product bioactivity.
Related Services
How to order?
