HPLC Analysis Service

High Performance Liquid Chromatography (HPLC) is a widely used analytical technique renowned for its high resolution, sensitivity, and speed. It is extensively applied in pharmaceutical development, biotechnology, environmental testing, and food safety. HPLC effectively separates and quantifies complex mixtures, playing a crucial role in scientific research and industrial production.

 

Services at MtoZ Biolabs

MtoZ Biolabs, a company specialized in life science analysis services, is committed to providing global research institutions and businesses with professional, efficient, and reliable HPLC analysis service. Utilizing advanced international HPLC systems and methods, we deliver rapid and precise analysis of target compounds and purity in samples. Our HPLC platform features high-end equipment from renowned brands such as Agilent, Waters, and Shimadzu, equipped with various detectors, including UV, diode array (DAD), fluorescence (FLD), and mass spectrometry (MS), addressing diverse analytical needs. Our HPLC Analysis Service encompasses purity analysis, content determination, impurity detection, and method development and validation, catering to pharmaceuticals, biologics, natural products, food additives, and pesticide residues.

 

Analysis Workflow

Clients can enjoy our professional and efficient HPLC analysis service through the following straightforward workflow:

1. Consultation: Discuss analysis requirements with our technical consultants to define objectives and methods.

2. Sample Submission: Clients prepare and submit samples according to our detailed guidelines.

3. Sample Pretreatment: Our technicians perform necessary pretreatment procedures such as dilution, filtration, and extraction based on sample characteristics.

4. HPLC Analysis: Employing advanced chromatography systems and standardized methods, we separate and analyze samples.

5. Data Processing: After acquiring chromatographic data, technicians conduct detailed analysis and processing.

6. Report Delivery: We provide comprehensive analytical reports, including chromatograms, methodology, result interpretation, and relevant recommendations.

 

Service Advantages

MtoZ Biolabs offers significant advantages in HPLC analysis service:

1. Professional and Reliable: International standard methods and advanced equipment ensure precise results.

 

2. Efficient and Rapid: Standardized process management delivers rapid analysis and timely results.

 

3. Comprehensive and Flexible: Multiple detection methods and various chromatographic columns cater to diverse analytical requirements.

 

4. Customized Service: Tailored method development and optimization based on specific client requirements.

 

5. High Data Security: Strict confidentiality and data protection measures ensure the security and integrity of client data.

 

Applications

Thanks to its powerful analytical capabilities, HPLC is widely utilized in:

1. Pharmaceutical R&D and Manufacturing: Determination of drug purity, impurity content, and pharmacokinetic studies.

 

2. Food Safety: Qualitative and quantitative analyses of food additives, nutrients, pesticide residues, and contaminants.

 

3. Environmental Testing: Monitoring water quality, analyzing soil contaminants, and detecting air pollutants.

 

4. Biopharmaceuticals and Natural Products: Analysis of protein, peptide, and natural product purity and composition.

 

5. Chemical Industry: Measurement of chemical intermediates' content and purity.

 

Case Study

1. Protein Purity Anaysis by HPSEC

 



Riguero, V. et al. J Chromatogr A. 2020.

Figure 1. Protein Purity Analysis by HPSEC

 

2. Developing a High-Performance Liquid Chromatography Fast and Accurate Method for Quantifcation of Silibinin

 



Bakhshi, F. et al. BMC Res Notes. 2019.

Figure 2. Silibinin Chromatogram in Aqueous Samples with a Concentration of 50 µg/mL

 

MtoZ Biolabs assists clients in rapidly obtaining critical data to support scientific research and quality control through our specialized HPLC analysis service.

 

FAQ

Q1. How much sample is required for HPLC analysis?

A: Typically, 1-10 mg of sample is sufficient, depending on complexity and target concentration.

 

Q2. Who handles sample pretreatment?

A: MtoZ Biolabs provides comprehensive pretreatment services including filtration, extraction, and dilution. Clients only need to submit raw samples.

 

Q3. What is the typical turnaround time for HPLC analysis?

A: Regular analysis takes 3-7 working days, depending on sample quantity and analysis complexity.

 

Q4. Do you offer method development and validation services?

A: Yes, our experienced technical team provides custom method development and validation tailored to your project needs.

 

Q5. What sensitivity levels can HPLC achieve?

A: Sensitivity typically reaches microgram or even nanogram levels, depending on sample characteristics, detection methods, and equipment.

 

Q6. How do you ensure data accuracy and reliability?

A: We implement rigorous quality control, including instrument calibration, method validation, blank controls, and standard analyses, to ensure data accuracy and reliability.

 

Through our professional HPLC analysis service, MtoZ Biolabs is dedicated to efficiently addressing analytical needs, supporting scientific research and product development. Please contact us anytime for further inquiries!