Fumarylation Proteomics Analysis Service
- Serum/Plasma: 100 µL
- Urine: 2 mL
- Animal Tissue: 200 mg
- Plant Tissue: 10 g
- Microorganisms: 500 µL
- Protein Extract: 5 mg
- Cells: 1×10⁸ cells
Fumarylation proteomics analysis is an advanced method used to study fumarylation, a post-translational modification, by detecting and quantifying the location and abundance of fumarylation in proteins through high-resolution techniques such as mass spectrometry. This modification plays a critical role in regulating cellular metabolism, signal transduction, and stress responses. Its importance in diseases such as cancer, metabolic disorders, and neurodegenerative conditions highlights the need for quantitative analysis of fumarylation-modified proteins to understand their biological significance in health and disease mechanisms.
MtoZ Biolabs provides precise Fumarylation Proteomics Analysis Service that identifies and quantifies fumarylation modifications on lysine residues in proteins. Our customized services support researchers worldwide by helping them explore the role of fumarylation in diverse biological and disease-related studies.
Services at MtoZ Biolabs
Utilizing Thermo Fisher’s Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos platforms, combined with Nano-LC systems, our Fumarylation Proteomics Analysis Service offers deep proteome analysis. It provides accurate identification of fumarylation sites and quantification of modification abundance, ensuring high sensitivity and precision, especially for large-scale research, including studies on disease mechanisms, metabolic regulation, and cancer.
We also offer targeted fumarylation analysis, focusing on specific proteins’ fumarylation modifications, which aids in exploring their roles in cancer, metabolic diseases, and neurodegenerative conditions, supporting target discovery and disease research.
Analysis Workflow
MtoZ Biolabs follows a standardized process for Fumarylation Proteomics Analysis Service to ensure reliable and reproducible data:
1. Sample Preparation
Proteins are extracted from cells, tissues, or body fluids using lysis buffer with proteinase inhibitors and demodification enzyme inhibitors, preserving fumarylation modifications.
2. Protein Digestion
Proteins are digested with trypsin, generating smaller peptides for enrichment and analysis.
3. Peptide Enrichment
Fumarylated peptides are selectively enriched using antibody-based or chemical methods, ensuring efficient separation and higher sensitivity in analysis.
4. Mass Spectrometry Analysis
Using LC-MS/MS technology, enriched fumarylated peptides are analyzed to identify modification sites accurately.
5. Data Analysis
We use specialized software like MaxQuant and Proteome Discoverer for data analysis, locating fumarylation sites and quantifying modification levels.
Figure 1. Fumarylation Proteomics Analysis Workflow
Why Choose MtoZ Biolabs?
✅ Advanced Analytical Platforms: Our Fumarylation Proteomics Analysis Service combines Thermo Fisher’s mass spectrometry platforms and Nano-LC systems, ensuring high-sensitivity, precise fumarylation analysis with reliable results.
✅ High-Quality Data: Data is rigorously controlled for quality, with AI-driven bioinformatics providing comprehensive reports.
✅ Extensive Coverage: By utilizing TMT/iTRAQ and Label-Free methods, we offer multi-sample quantitative analysis, improving experimental efficiency and ensuring reliable data.
✅ Customized Services: We offer tailored experimental designs and workflows to meet the specific needs of various research projects.
✅ Transparent Pricing: We offer transparent pricing with no hidden fees, providing fair and reasonable services.
Sample Submission Suggestions
To ensure efficient and accurate fumarylation proteomics analysis, we recommend the following sample types and quantities:
1. Sample Type and Amount
2. Sample Shipping
Lyophilized samples can be shipped at room temperature. Liquid samples should be shipped on ice or with dry ice. Fresh tissue samples must be transported with dry ice or refrigeration.
For special or limited samples, please contact us in advance for feasibility confirmation. MtoZ Biolabs also provides detailed sample submission guidelines via technical support.
What Could be Included in the Report?
1. Detailed Report: Includes experimental methods, materials list, and a detailed explanation of experimental procedures.
2. Data Analysis Report: Provides fumarylation site identification, quantification, and bioinformatics analysis results.
3. Quality Control Report: Ensures data quality through Total Ion Chromatogram (TIC) and quality control assessments.
4. Raw Data Files: Provides raw mass spectrometry data for further analysis.
For further information or to explore customized fumarylation analysis, please contact MtoZ Biolabs. We offer expert Fumarylation Proteomics Analysis Services to support your research and product development. We also provide other PTM proteomics services such as phosphorylation, glycosylation, acetylation, and ubiquitination to help you comprehensively study protein regulation across various dimensions.
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