Fructooligosaccharide Analysis Service
Fructooligosaccharides (FOS) are functional oligosaccharides composed of 2 to 10 fructose units linked by β-1,2-glycosidic bonds, and are widely found in plants such as onions, garlic, and chicory. As a natural prebiotic, FOS can promote the growth of beneficial bacteria, improve gut health, enhance immune function, and have effects such as regulating lipid metabolism and antioxidant activity. Due to their excellent bioactivity and stability, FOS are widely applied in the fields of food, dietary supplements, feed additives, and biomedicine.
Bhadra, S. et al. Bioresource Technology Reports, 2022.
Figure 1. (a) FOS General Structure; and 1-kestose (GF2) (A), Nystose (GF3) (B) and
Services at MtoZ Biolabs
Based on a high-resolution chromatography-mass spectrometry (LC-MS/MS) platform, MtoZ Biolabs offers the fructooligosaccharide analysis service which provides comprehensive qualitative and quantitative analysis of fructooligosaccharides. This service can analyze the content, purity, structural characteristics, and degree of polymerization distribution of fructooligosaccharides in samples, and identify different modification forms (such as acetylation). Through efficient separation and sensitive detection, the data obtained helps evaluate the quality and composition of fructooligosaccharides, providing support for the research and product optimization of functional oligosaccharides. MtoZ Biolabs offers services including but not limited to the following:
1. Quantitative Analysis
Using high-performance liquid chromatography (HPLC) and mass spectrometry (LC-MS/MS) techniques, the content of fructooligosaccharides is precisely measured to ensure data accuracy.
2. Purity Analysis
High-resolution separation techniques are used to evaluate the purity and impurities in the fructooligosaccharide sample, providing a basis for quality control.
3. Structural Characterization
High-resolution mass spectrometry (HR-MS) and nuclear magnetic resonance (NMR) are used to elucidate the molecular structure and modification features of fructooligosaccharides.
4. Degree of Polymerization Distribution
The distribution of fructooligosaccharides with different degrees of polymerization (DP) is analyzed, revealing structural complexity and functional differences.
5. Stability Evaluation
Changes in the structure and content of fructooligosaccharides under different storage conditions are monitored to assess their physicochemical stability and suitability for application.
6. Metabolic Analysis
Integrating metabolomic strategies, the metabolic pathways and dynamic changes of fructooligosaccharides in biological systems are studied.
Sample Submission Suggestions
1. Sample Types
Various types of samples are accepted, including plant extracts, fermentation broths, and purified fructooligosaccharide preparations. Liquid, powder, and lyophilized forms are all supported.
Note: Samples should be free from high salt, protein, or organic solvent residues to avoid interference with the accuracy of the results.
2. Sample Storage
Samples should be sealed and stored in the dark. For short-term storage, samples can be kept at -20°C, while long-term storage is recommended at -80°C. Avoid repeated freeze-thaw cycles to prevent oligosaccharide degradation.
3. Sample Transportation
Liquid samples should be transported on dry ice using a cold chain system. Lyophilized samples can be shipped at ambient temperature for short durations but must be protected from moisture, high temperatures, and light to ensure the stability of fructooligosaccharides.
Service Advantages
1. High-Resolution Analytical Platform
Leveraging an advanced LC-MS/MS system, the service ensures high-sensitivity detection and precise quantification of fructooligosaccharides, guaranteeing reliable and trustworthy results.
2. Wide Detection Range
The service can simultaneously analyze fructooligosaccharides with different degrees of polymerization, substitution types, and structural isomers, meeting the needs of various sample types.
3. One-Stop Service Process
Providing comprehensive technical support from sample preparation, detection analysis to data interpretation and report generation, offering an efficient and convenient integrated service experience.
4. Strict Quality Control System
Standard calibration and quality control monitoring are implemented throughout the process to ensure the accuracy and reproducibility of the detection results.
Applications
1. Plant Source Analysis
By identifying the composition and structural differences of fructooligosaccharides in various plant materials, this service helps evaluate extraction efficiency and raw material characteristics.
2. Metabolic Mechanism Research
The fructooligosaccharide analysis service can be used to explore the role of fructooligosaccharides in sugar and lipid metabolism as well as signal regulation, revealing their metabolic and physiological functional mechanisms.
3. Biomass Conversion
By studying the production mechanisms of fructooligosaccharides in lignocellulose and agricultural by-product conversion, this service supports the development of bio-based materials and energy.
4. Prebiotic Formulation Design
The fructooligosaccharide analysis service can be used to compare the prebiotic effects of fructooligosaccharides with different structures and degrees of polymerization, optimizing functional oligosaccharide formulations.
FAQ
Q1: Can the Analysis Identify Different Substitution Groups or Modification Forms of Fructooligosaccharides?
A1: Yes. By analyzing the fragment ions in high-resolution mass spectrometry, we can identify the substitution groups (such as acetylation, methoxylation) and terminal modification types of fructooligosaccharides, providing precise structural information.
Q2: Is Fructooligosaccharide Analysis Suitable for Integration with Metabolomics?
A2: Yes. The fructooligosaccharide data can be integrated with metabolite profiles or microbiome data, revealing its role in carbon source metabolism, microbial regulation, and energy conversion mechanisms.
Q3: Does the Analysis Account for Matrix Effects?
A3: Yes. All detection processes are calibrated with internal standards and blank matrix validation to eliminate ion suppression effects from complex samples, ensuring reliable data.
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