FT-IR for Protein and Peptide Analysis Service

Fourier transform infrared (FT-IR) spectroscopy is a powerful technique for obtaining the infrared spectra of absorption/emission in solids, liquids, or gases. When infrared radiation passes through a test sample, a part of the infrared radiation is absorbed by specific covalent bonds of functional groups, while another part of the infrared radiation directly penetrates through. The collected spectra represent the absorption and transmission of molecules, forming a molecular fingerprint for chemical identification. This also enables infrared spectroscopy to be used for various types of analysis. FT-IR spectrometers collect high-resolution spectra across a wide wavelength range simultaneously, which has significant advantages over dispersion spectrometers that can only measure peak intensities within a considerably narrow wavelength range.



Fonte: Wikipédia. 2010.

Figure 1. Principle of FT-IR Spectrometer

 

FT-IR spectrometers can be used for all applications where dispersion instruments are used to improve sensitivity and speed, able to outperform infrared spectroscopy analysis methods such as dispersion or filter methods depending on its: non-destructive nature, no need for external calibration, faster speed, higher sensitivity, higher light flux, and simpler operation.

 

Applications

1. Chemical Identification based on Spectral Differences of Homologs, Geometric, and Optical Isomers

2. Identification of Functional Groups in the Tested Chemicals based on the Absorbed Wavelength

3. Purity Estimation by Studying the Peak Values of Potential Contaminants

4. Tracking Chemical Reactions by Comparing Peaks of Specific Functional Groups

5. Quantitative Analysis of Chemicals by Monitoring Specific Peaks

 

MtoZ Biolabs uses Thermo company's Nicolet series instruments to establish an FT-IR analysis platform, determining the infrared spectra of proteins and peptides in samples, and performs subsequent baseline correction, Gaussian deconvolution, second derivative fitting, ultimately determining the secondary structure information of proteins and peptides based on peak area.

 

Service at MtoZ Biolabs Biologics Characterization