Extracellular Vesicles Metabolomics Services
Extracellular Vesicles Metabolomics Services at MtoZ Biolabs provide comprehensive solutions for the identification, quantification, and functional analysis of metabolites encapsulated within extracellular vesicles (EVs). These small vesicles, which include exosomes, microvesicles, migrasomes, and apoptotic bodies, are secreted by various cell types into multiple biological fluids. EVs carry bioactive molecules such as proteins, lipids, and RNAs, offering a snapshot of the physiological and pathological states of their parent cells. Metabolites, as key components of small molecule signaling, are particularly important. With molecular weights typically under 2 kDa, they encompass amino acids, steroid hormones, lipids, and intermediates of energy metabolism. Metabolites are crucial for studying cellular metabolic dynamics, disease mechanisms, and identifying potential therapeutic targets.
Services at MtoZ Biolabs
To support global researchers in exploring EV metabolic signatures, MtoZ Biolabs combines state-of-the-art mass spectrometry platforms with an experienced metabolomics team, providing one-stop EV metabolomics service. Our Extracellular Vesicles Metabolomics Services include both targeted and untargeted strategies, ensuring high-resolution, high-sensitivity metabolite detection and quantification across different sample backgrounds.
1. Targeted Metabolomics Services
Targeted metabolomics focuses on the quantitative analysis of specific metabolites or metabolic pathways. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we employ advanced techniques such as Multiple Reaction Monitoring (MRM) and Parallel Reaction Monitoring (PRM) for high-selectivity metabolite quantification and qualitative analysis.
We establish standard curves using high-quality standards and incorporate endogenous or isotope-labeled internal standards to achieve absolute quantification. This service is ideal for validating candidate biomarkers, studying drug metabolism pathways, and uncovering mechanistic targets.
2. Untargeted Metabolomics Services
Untargeted metabolomics offers a comprehensive exploration of all small molecule metabolites in EVs. Utilizing ultra-high-performance liquid chromatography (UHPLC) combined with high-resolution mass spectrometry platforms like Orbitrap Fusion Lumos, we can analyze a broad range of metabolites.
Through high-throughput scanning modes, we capture the full metabolome, followed by principal component analysis (PCA), differential metabolite screening, hierarchical clustering, and pathway enrichment analysis to uncover potential biological associations. Relative quantification is typically performed using peak area ratios or internal standard correction, enabling accurate comparison of metabolite abundance. This service is ideal for discovering new disease biomarkers, investigating changes in cellular states, and exploring disease mechanisms.
Figure 1. Workflow for Extracellular Vesicles Metabolomics
Why Choose MtoZ Biolabs?
· Advanced Analysis Platform
Our advanced instruments, including the Orbitrap Fusion Lumos and Q Exactive, are compatible with PRM, MRM, and other analysis modes, ensuring high sensitivity and specificity for low-abundance metabolites.
· Flexible Quantification Strategies
We offer both absolute quantification (via standard curves) and relative quantification (using internal standards or peak area ratios), catering to a wide variety of research needs.
· Comprehensive Bioinformatics Support
Our bioinformatics platform supports data interpretation with PCA, differential metabolite screening, pathway enrichment, and visualization, enabling detailed insights into the metabolome.
· Versatile Sample Compatibility
Our Extracellular Vesicles Metabolomics Services support EVs from plasma, serum, urine, cerebrospinal fluid, cell culture supernatant, and in vitro cultured cells, making it adaptable to diverse research settings.
· End-to-End Customizable Workflow
We provide complete solutions, from EV enrichment and mass spectrometry detection to downstream bioinformatics analysis, minimizing research costs while ensuring high-quality results.
Applications
✔ Early Disease Diagnosis and Prognostic Biomarker Discovery: Identify EV-derived biomarkers for early diagnosis and monitoring of diseases such as cancer, neurodegenerative disorders, and metabolic diseases.
✔ Cell Communication Mechanisms and Pathological Process Analysis: Uncover the role of EVs in mediating intercellular signaling and regulating pathological processes in disease contexts.
✔ Cancer, Neurodegenerative Disease, and Metabolic Disease Research: Investigate metabolic changes in EVs to understand disease mechanisms and uncover potential therapeutic targets.
✔ Personalized Medicine and Targeted Therapeutic Strategy Development: Utilize EV metabolomics to design personalized treatment plans and identify novel therapeutic targets.
✔ Multi-Omics Integration and Systems Biology Research: Integrate metabolomics with other omics data (proteomics, transcriptomics) for comprehensive modeling and systems-level analysis of biological processes.
FAQ
Q1: What are the specific advantages of MRM and PRM in EV metabolite analysis?
Both MRM and PRM offer exceptional selectivity and sensitivity, making them ideal for quantifying low-abundance metabolites in complex matrices. MRM allows for high-throughput, multi-target analysis, while PRM, when used with Orbitrap platforms, provides high-resolution full scans for precise peak identification and traceability. We select the most appropriate method based on the complexity of your samples and the metabolites of interest.
Q2: Can metabolomics data be integrated with proteomics or transcriptomics data for a more comprehensive analysis?
Yes, MtoZ Biolabs provides multi-omics integration capabilities, enabling the combination of EV metabolomics data with proteomics, transcriptomics, and miRNA data. This integration allows for a holistic view of the regulatory mechanisms and biological pathways involved, supporting advancements in precision medicine and systems biology.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
At MtoZ Biolabs, we are committed to providing high-quality Extracellular Vesicles Metabolomics Services to help uncover cellular metabolic states and disease mechanisms under different environmental conditions. In addition to extracellular vesicles metabolomics, we also offer comprehensive omics services for various organelles, including mitochondria, Golgi apparatus, endoplasmic reticulum, lysosomes, and peroxisomes. Our services cover organelle isolation, proteomics, metabolomics, and multi-omics integration, helping researchers gain deep insights into the molecular functions of subcellular components.
Contact us today to learn more about our Extracellular Vesicles Metabolomics Services or to discuss customized research solutions tailored to your specific needs.
Related Services
Extracellular Vesicles Analysis Services
Extracellular Vesicles Proteomics Services
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