Extracellular Vesicles Proteomics Services
Extracellular Vesicles Proteomics Services provide comprehensive solutions for the identification, quantification, and functional annotation of proteins encapsulated within extracellular vesicles (EVs). EVs encompass various subtypes—such as exosomes, microvesicles, migrasomes, and apoptotic bodies—and are secreted by nearly all cell types into diverse biological fluids. These vesicles carry bioactive molecules including proteins, lipids, and RNAs from donor cells, acting as critical messengers in intercellular communication, immune modulation, and disease pathogenesis.
Among EV cargo, proteins play pivotal roles as signaling mediators in biological processes such as inflammation, apoptosis, autophagy, and angiogenesis. They are also gaining increasing attention as potential disease biomarkers and therapeutic targets. MtoZ Biolabs offers sensitive, high-coverage Extracellular Vesicles Proteomics Services by leveraging high-resolution mass spectrometry and integrated multi-omics capabilities. Our analyses cover global protein profiling, quantitative expression analysis, and post-translational modification (PTM) characterization—empowering discoveries in regenerative medicine, immunotherapy, oncology, and beyond.
Services at MtoZ Biolabs
Powered by advanced LC-MS/MS systems and validated EV handling protocols, MtoZ Biolabs offers a full spectrum of Extracellular Vesicles Proteomics Services:
· EV Protein Extraction and Identification
We isolate high-purity EV samples using optimized techniques such as density gradient ultracentrifugation, immunoaffinity capture, microfluidics, or nanomaterials, followed by LC-MS/MS for comprehensive protein identification and novel protein discovery.
· EV Marker and Transmembrane Protein Profiling
Identification of canonical markers (e.g., CD9, CD63, CD81, TSG101, ALIX) and structural proteins for validating sample origin and confirming vesicle subtype enrichment.
· Quantitative Proteomics
TMT/iTRAQ labeling or label-free quantification enables accurate comparative analysis across multiple conditions or sample groups, supporting differential expression studies and trend evaluation.
· Post-Translational Modification (PTM) Analysis
We offer in-depth profiling of PTMs such as phosphorylation, glycosylation, ubiquitination, and methylation to reveal regulatory mechanisms and signaling pathways active within EVs.
Analysis Workflow
MtoZ Biolabs follows a standardized and customizable analytical pipeline to ensure data accuracy, reproducibility, and biological relevance:
1. Strategy Design Based on Sample Profile
Selection of optimal EV isolation and protein extraction protocols based on sample type (e.g., cell culture supernatant, plasma, urine, cerebrospinal fluid).
2. EV Enrichment and Protein Processing
EVs are purified using ultracentrifugation, density gradients, affinity-based enrichment, or microfluidic platforms, followed by gentle lysis to extract protein content.
3. Mass Spectrometry Profiling
Protein profiling is performed using Orbitrap Fusion Lumos or Q Exactive instruments under DDA, DIA, or PRM modes for precise data collection.
4. Bioinformatics Analysis
Includes protein quantification, differential analysis, GO/KEGG enrichment, PTM site localization, and protein–protein interaction network reconstruction.
Why Choose MtoZ Biolabs?
✅ Multi-Platform Mass Spectrometry Coverage: Supports DDA, DIA, and PRM acquisition strategies to meet diverse experimental requirements.
✅ Expertise in EV Subtype Processing: Specialized workflows for exosomes, microvesicles, and apoptotic bodies ensure accurate subtype-specific proteomic profiling.
✅ Integrated PTM Characterization: Simultaneous identification of multiple PTM types enables functional protein insight beyond expression level alone.
✅ Cross-Omics Integration Capability: Seamless incorporation with transcriptomics, metabolomics, and lipidomics for multi-layered systems biology analysis.
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Applications
· Biomarker Discovery: Identify EV protein signatures associated with cancer, neurodegenerative diseases, autoimmune disorders, and other pathologies.
· Immunology and Inflammation Research: Investigate the role of EVs in immune cell communication and regulation of inflammatory pathways.
· Stem Cell-Derived EV Functional Studies: Explore protein-based mechanisms involved in tissue regeneration, cell repair, and paracrine signaling.
· Drug Delivery System Development: Characterize EV protein composition to support their use as natural carriers for RNA therapeutics, vaccines, or targeted agents.
· Mechanistic and Pathway Analysis: Use protein interaction networks and PTM mapping to reconstruct molecular mechanisms underlying disease processes.
FAQ
Q1: Can you support high-throughput proteomic analysis and quantitative comparisons across multiple EV samples?
Yes. MtoZ Biolabs supports high-throughput proteomics using TMT and iTRAQ labeling, allowing for simultaneous analysis of 8–18 samples with excellent quantification precision. For projects with limited sample material or budget constraints, we also offer robust label-free quantification alternatives.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
Whether you're exploring the biological roles of EV proteins, developing disease biomarkers, or engineering EV-based delivery systems, MtoZ Biolabs provides the analytical expertise and platform infrastructure to drive your discoveries forward. Our Extracellular Vesicles Proteomics Services are fully customizable to accommodate diverse research goals and sample types.
Beyond EVs, MtoZ Biolabs also offers organelle-focused omics services covering mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, and peroxisomes. We provide integrated solutions—from organelle isolation and purification to proteomics, metabolomics, and multi-omics integration—supported by advanced instrumentation and a dedicated bioinformatics team.
Contact us today to explore our full suite of subcellular omics services and accelerate your journey in functional systems biology.
Related Services
Extracellular Vesicles Analysis Services
How to order?
