Exosome-Mediated mRNA Delivery Service

Exosomes are nanoscale membrane-bound vesicles secreted by cells. They possess excellent biocompatibility, low immunogenicity, and intrinsic transmembrane transport capabilities. Exosomes can carry a variety of bioactive molecules such as mRNA, miRNA, and proteins, enabling intercellular material transfer. Exosome-mediated mRNA delivery utilizes exosomes as natural delivery vehicles to encapsulate and transport functional mRNA into target cells, thereby enhancing mRNA stability and translational efficiency while providing an efficient and safe transport solution for mRNA research.

 

Exosome-mediated mRNA delivery service is widely applied in fields such as cellular reprogramming, protein function studies, evaluation of mRNA stability and expression efficiency, and the exploration of signaling pathway regulation mechanisms. Leveraging a mature exosome isolation and mRNA loading platform, MtoZ Biolabs provides end-to-end services to support researchers in efficiently conducting mRNA delivery-related studies.

 



Yao, T. et al. International Journal of Biological Macromolecules, 2025.

Figure 1. Biogenesis, Secretion and Uptake of Exosomes.

 

Services at MtoZ Biolabs

Leveraging an advanced technology platform, MtoZ Biolabs has launched the exosome-mediated mRNA delivery service focusing on the use of exosomes as carriers for mRNA delivery. The service encompasses exosome isolation and purification, mRNA loading, delivery system construction, and evaluation of delivery efficiency to target cells. Using techniques such as fluorescence microscopy, we help clients systematically assess the cargo-loading efficiency and delivery capacity of exosomes, uncovering their roles in intercellular communication and supporting a broad range of biomedical and basic research applications.

 

Analysis Workflow

1. Exosome Isolation and Purification

High-purity exosomes are efficiently isolated from samples using ultracentrifugation, SEC, or ultrafiltration methods.

 

2. mRNA Loading

Target mRNA is loaded into exosomes via electroporation or chemical transfection, with subsequent evaluation of loading efficiency.

 

3. Delivery Efficiency Assessment

The delivery performance of exosome-mediated mRNA transfer is validated in target cells using fluorescence labeling, RT-qPCR, and other quantitative techniques.

 

4. Data Analysis and Reporting

Loading efficiency, delivery efficacy, and target cell expression data are integrated to generate a standardized analytical report supporting further experimental design and optimization.

 



Alsan, C. et al. BMC Biotechnology, 2021.

Figure 2. mRNA-Encapsulated Exosomes.

 

Sample Submission Suggestions

1. Sample Types

Exosome samples derived from cell culture supernatant, serum/plasma, urine, etc., are accepted. Purified exosomes or target mRNA molecules can also be submitted. For cell samples, please specify the culture medium composition and treatment conditions.

 

2. Sample Transportation

Samples should be centrifuged to remove cell debris, stored at −80°C, and shipped on dry ice to prevent repeated freeze-thaw cycles.

 

3. Sample Information

Please provide detailed background information, including sample origin, processing methods, and whether any exogenous labeling is involved, to help us develop the optimal analysis plan.

 

Service Advantages

1. Advanced Analytical Platforms

Equipped with high-resolution mass spectrometry, ultracentrifugation, NTA, and other technologies to ensure analytical accuracy and reliability.

 

2. Experienced Research Team

Our team has extensive experience in exosome research, proficient in handling various sample types and well-versed in the entire process of exosome loading and delivery.

 

3. Customized Services

Flexible experimental design tailored to clients' research needs, supporting diverse scientific objectives.

 

4. One-Stop Service

Comprehensive support from sample processing to data analysis, streamlining experimental procedures and accelerating research progress.

 

Applications

1. Exosome Delivery Efficiency Evaluation

Exosome-mediated mRNA delivery service can be used to evaluate the efficiency, stability, and tissue specificity of exosomes derived from different cell sources during mRNA loading and delivery, providing data support for subsequent optimization of delivery systems.

 

2. Cellular Uptake and Functional Response Verification

By analyzing the uptake efficiency and expression outcomes of exosome-loaded mRNA in target cells, this service helps validate the potential application of exosomes in modulating cellular functions.

 

3. Mechanistic Study of mRNA Delivery

Exosome-mediated mRNA delivery service supports investigation into the underlying mechanisms of exosome-based mRNA delivery, including membrane fusion, endocytosis pathways, and intracellular release, offering experimental evidence for understanding delivery behavior.

 

4. Development and Evaluation of Delivery Systems

This service provides a technical evaluation platform for developing new delivery systems, facilitating the screening of efficient and safe exosome-based carrier platforms.

 

FAQ

Q1: Are Exosome or mRNA Samples Provided by Customers Supported?

A1: Yes. Clients may submit pre-isolated exosomes or mRNA, and we will develop an optimized loading and delivery strategy based on the sample characteristics.

 

Q2: Can the in Vivo Distribution of Exosomes Be Tracked?

A2: If required, we can assist clients with exosome tracking analysis using fluorescent dye labeling or other tracing techniques.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files