Exosome-Mediated miRNA Delivery Service

Exosomes are nanoscale membrane-bound vesicles secreted by cells, characterized by excellent biocompatibility, low immunogenicity, and efficient transmembrane delivery capacity. They are widely involved in intercellular communication. MicroRNAs (miRNAs) are small non-coding RNAs approximately 22 nucleotides in length that regulate gene expression by targeting mRNAs. MtoZ Biolabs offers high-purity exosome isolation and purification services, along with efficient miRNA loading strategies, to support the construction of stable and reliable exosome-based miRNA delivery systems.

 

Exosome-mediated miRNA delivery service utilizes exosomes as natural carriers to encapsulate functional miRNAs for downstream delivery studies. This service is widely applicable to various research contexts, including miRNA functional studies, signaling pathway regulation, validation of miRNA roles in disease models, and modulation of cellular phenotypes. Exosome-mediated miRNA delivery enables targeted, low-toxicity interventions, providing a powerful tool for molecular mechanism exploration and biological function validation.

 



Fang, Z W. et al. CCL, 2022.

Figure 1. Application of Exosomes as miRNA Drug Carriers.

 

Services at MtoZ Biolabs

Based on advanced analytical platforms, MtoZ Biolabs has launched the exosome-mediated miRNA delivery service focusing on exosome-based miRNA delivery. We utilize electroporation and chemical transfection methods to load miRNAs into high-purity exosomes, and employ quantitative approaches to assess loading efficiency. This service provides high-quality carrier preparation to support subsequent cellular delivery experiments, facilitating miRNA functional research and delivery system development.

 

Analysis Workflow

1. Exosome Isolation and Purification

High-purity exosomes are efficiently extracted from cell culture supernatants or biofluid samples using techniques such as ultracentrifugation, size exclusion chromatography (SEC), or ultrafiltration.

 

2. miRNA Loading

Target miRNAs are efficiently loaded into exosomes via electroporation, electrotransfection, or chemical transfection methods. Loading efficiency is quantitatively evaluated to ensure reproducibility.

 

3. Quality Validation

Post-loading validation includes assessment of particle size distribution and miRNA loading levels to ensure the stability and consistency of the delivery system.

 



Foo, B J. et al. Pharmaceuticals, 2021.

Figure 2. miRNA Rnrichment Can Be performed Via (A) Cell Modification or (B) Direct Loading of miRNAs into Exosomes.

 

Sample Submission Suggestions

1. Sample Types

We accept liquid-based samples such as cell culture supernatants and serum/plasma, as well as customer-provided purified exosomes or target miRNA molecules.

 

2. Sample Transportation

Samples should be pre-cleared by low-speed centrifugation to remove cellular debris, stored at −80°C, and shipped on dry ice to preserve exosome integrity and avoid freeze-thaw cycles.

 

3. Information Note

Please provide comprehensive background information, including sample origin, processing methods, and whether exogenous labels were used, to optimize the subsequent miRNA loading strategy and workflow.

 

Service Advantages

1. Advanced Analytical Platforms

Equipped with ultracentrifugation and nanoparticle tracking analysis (NTA), ensuring high efficiency and purity in exosome isolation and purification.

 

2. Extensive Exosome Research Experience

Skilled in handling various sample types and proficient in key steps of exosome extraction and miRNA loading, ensuring stable and reliable service quality.

 

3. Customized Workflow

Tailored experimental strategies based on specific sample characteristics and research goals to meet diverse scientific needs.

 

4. Efficient One-Stop Service

From sample preparation to miRNA loading, MtoZ Biolabs offers a streamlined and complete workflow to simplify operations and enhance experimental efficiency.

 

Applications

1. Cell Function Regulation Studies

Exosomes carrying miRNAs can influence gene expression in recipient cells, enabling investigation into the regulatory mechanisms of key biological processes such as cell proliferation, differentiation, and apoptosis.

 

2. Disease Model Studies

Exosome-mediated miRNA delivery service can be used to construct exosomes loaded with specific miRNAs to mimic intercellular communication under disease conditions, contributing to the elucidation of the pathogenesis of various diseases.

 

3. Signal Pathway Regulation Analysis

By delivering specific miRNAs to target cells, this service supports the study of their effects on the activation or inhibition of key signaling pathways, helping to decode intracellular and extracellular regulatory networks.

 

4. Exosome Carrier Screening and Evaluation

Exosome-mediated miRNA delivery service can be used to evaluate the differences among exosomes from various sources in terms of miRNA loading efficiency, targeting capability, and functional expression, thereby optimizing the delivery system.

 

FAQ

Q1: Can I Submit Only miRNA or Exosome Samples?

A1: Yes. We accept client-provided miRNA or exosome samples and will tailor the optimal loading strategy based on the specific sample conditions.

 

Q2: Do you Support Confidentiality Agreements for miRNA Sequences?

A2: Yes. We strictly comply with client confidentiality agreements to ensure the security of all sequence information and experimental data.

 

Q3: Can I Use Specifically Labeled Exosomes for Tracking Purposes?

A3: We can assist with exosome labeling upon request. Please inform us of your experimental needs in advance so we can assess feasibility and provide a compatible solution.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Data Analysis, Preprocessing, and Estimation

4. Bioinformatics Analysis

5. Raw Data Files