ESI-MS Peptide Molecular Weight Identification Service
- Raw ESI-MS data files
- Excel or CSV result tables
- PDF analytical report containing methods and key conclusions
- High-resolution spectra in TIFF or PNG format
- Custom formats can be provided based on project requirements.
MtoZ Biolabs provides a professional ESI-MS peptide molecular weight identification service based on the electrospray ionization mass spectrometry (ESI-MS) platform to achieve rapid and accurate determination of peptide molecular weights. This service is widely applied in peptide synthesis validation, modification analysis, protein digestion product identification, and quality control. It provides high-sensitivity and high-resolution analytical data for research institutes, pharmaceutical companies, and biotechnology enterprises to accelerate peptide research and product development.
Principle of ESI-MS
ESI-MS is a liquid-phase-based soft ionization mass spectrometry technique suitable for detecting peptides, proteins, nucleic acids, and other polar biomolecules. The principle involves applying a high voltage to the liquid sample to generate charged droplets. As the solvent evaporates, the remaining charged molecules are transferred into the mass spectrometer for analysis. This process requires no high temperature or matrix assistance, avoiding fragmentation and maintaining molecular integrity.
After entering the mass spectrometer, ions are recorded by the detector according to their mass-to-charge ratio (m/z), from which the precise molecular weight is calculated. Because ESI produces multiply charged ion signals, deconvolution algorithms are used to determine the neutral molecule’s true mass. Compared with MALDI, ESI is more suitable for liquid-phase and complex samples, and it can be directly coupled with HPLC or UPLC systems to enable high-sensitivity and high-throughput continuous analysis.
Lavigne, JP. et al. Clin Chem Lab Med. 2013.
Figure 1. Principle of ESI MS
ESI-MS Peptide Molecular Weight Identification Service at MtoZ Biolabs
The ESI-MS peptide molecular weight identification service at MtoZ Biolabs covers the complete process from sample preparation to data reporting and is suitable for various peptide types. Using high-resolution electrospray ionization mass spectrometers such as Thermo Fisher Orbitrap and Q Exactive series, we provide accurate molecular weight measurements and spectrum interpretation for clients.
Sample types available for analysis include:
1. Synthetic Peptides: Used to verify sequence accuracy and purity of artificially synthesized peptides.
2. Natural Peptides: Detect molecular weight composition of peptides derived from biological tissues or extracts.
3. Enzymatic Peptides: Applied in peptide mass fingerprinting and protein identification.
4. Modified and Labeled Peptides: Analyze molecular weight variations caused by phosphorylation, acetylation, methylation, or isotopic labeling.
5. Peptide Drugs and Biologics: Verify structural integrity and quality consistency of therapeutic peptides and their derivatives.
Through optimized electrospray ionization conditions and high-resolution detection systems, MtoZ Biolabs obtains high signal-to-noise ratio spectra with minimal sample quantity, achieving precise molecular weight measurement and structural confirmation.
Workflow of ESI-MS Peptide Molecular Weight Identification Service
Why Choose MtoZ Biolabs
1. High-Resolution Analytical Platforms: Equipped with Thermo Fisher Q Exactive and Orbitrap ESI-MS systems, ensuring precise and reliable results.
2. Customized Analytical Strategies: Tailored methods designed according to sample characteristics and research objectives.
3. Broad Sample Compatibility: Suitable for peptides, proteins, and complex biological samples.
4. Strict Quality Control: Comprehensive QC and standard comparison ensure accuracy and traceability.
5. Rapid Turnaround: Optimized workflow supports high-throughput testing and fast report delivery.
Applications of ESI-MS Peptide Molecular Weight Identification Service
1. Peptide Synthesis Validation: Confirm sequence accuracy and modification completeness of synthetic peptides.
2. Protein Digestion Analysis: Used for peptide mass fingerprinting and protein identification.
3. Modified Peptide Research: Identify modification types and evaluate their impact on molecular weight.
4. Drug Quality Control: Assess structural consistency of peptide-based pharmaceuticals and biologics.
5. Metabolomic and Functional Research: Confirm structures and functions of peptide metabolites.
Sample Submission Guidelines
MtoZ Biolabs can provide detailed sample submission guidelines and pretreatment recommendations upon request to ensure the stability and reliability of analytical results.
Deliverables
1. Standardized experimental report
2. Original ESI-MS spectra and deconvolution results
3. Peptide molecular weight tables and main ion peak data
4. Signal intensity, isotope distribution, and peak area statistics
5. PDF report suitable for publication or quality validation
FAQs
Q1: What Is the Service's General Workflow?
Our ESI-MS peptide molecular weight identification service supports synthetic peptides, natural peptides, enzymatic peptides, modified peptides, and therapeutic peptides. MtoZ Biolabs optimizes analytical conditions for different sample matrices to ensure stable signals and accurate outcomes.
Q3: What Data Formats Are Provided?
Multiple standard formats are available for analysis and visualization, including:
Q4: How Should I Prepare My Samples?
Samples should be free of high salts or strong acid/base environments. Volatile buffers such as 0.1% formic acid or acetonitrile are recommended. Store samples below −20°C and ship on dry ice.
For more information, please refer to Sample Submission Guidelines for Proteomics and Sample Submission Guidelines for Metabolomics.
If you are unsure about your sample preparation, our technical team can provide personalized pre-analysis consultation and a sample submission guide to help you optimize preparation and shipping conditions.
Start Your Project with MtoZ Biolabs
Contact us to discuss your project or request a quote. Whether you are validating synthetic peptides, analyzing enzymatic digestion products, or characterizing therapeutic peptide formulations, MtoZ Biolabs is ready to support your research with accurate, efficient, and customized ESI-MS peptide molecular weight identification solutions.
How to order?
