DIA-Based Peptide Quantification Service
-
Comprehensive detection of peptide signals, reducing the omission of low-abundance peptides
-
Strong cross-sample quantitative consistency, suitable for large-batch analysis
-
Supports precise quantification based on spectral-library and library-free approaches
-
Applicable to differential analysis, pathway studies, and drug effect evaluation
-
RAW mass spectrometry data files;
-
DIA quantification result tables (Excel format);
-
Quantitative spectra, peak area plots, and related visual graphics (TIFF or PNG format);
-
Comprehensive analysis report (PDF), including experimental conditions and result interpretation.
MtoZ Biolabs uses a high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS) platform to provide the DIA-based peptide quantification service which enables high-throughput and comprehensive quantitative analysis of peptides in complex samples. This service is suitable for studies of protein expression differences, biomarker screening, and multi-condition sample comparison, providing a reliable data foundation for systematic peptide quantification research.
Principle of Data-Independent Acquisition
Data-independent acquisition (DIA) is a mass spectrometry acquisition mode in which all ions within a defined mass range are fragmented and detected simultaneously, allowing comprehensive capture of peptide information from a sample. Compared with the DDA strategy that selects only a portion of ions, DIA offers higher coverage and stronger reproducibility, reducing the omission of low-abundance peptides. In peptide quantification, DIA uses wide or variable acquisition windows to collect full-spectrum data, then applies spectral-library-based or library-free algorithms to accurately compare peptide abundance across samples, providing a stable and reliable technical basis for large-scale quantitative studies.
Goetze, S. et al. Clinical Proteomics, 2024.
Figure1. Diagram of DIA Acquisition Mode.
DIA-Based Peptide Quantification Service at MtoZ Biolabs
MtoZ Biolabs uses mass spectrometry technology based on data-independent acquisition (DIA) to provide high-coverage and highly consistent large-scale peptide quantification services, enabling accurate evaluation of peptide abundance changes under different conditions.
Our analysis offers systematic insights into peptide expression changes, helping researchers gain a deeper understanding of key biological processes and regulatory mechanisms.
Workflow of DIA-Based Peptide Quantification Service
1. Sample Preparation and Peptide Generation
Proteins are extracted, digested, and purified to obtain high-quality peptides suitable for DIA analysis.
2. DIA Mass Spectrometry Data Acquisition
A high-resolution DIA mode is used to comprehensively collect all ion information, ensuring high coverage and strong reproducibility of peptide signals.
3. Data Processing and Quantification
Library-based or library-free software is applied for feature extraction, quantitative comparison, and differential analysis to achieve accurate cross-sample quantification.
4. Result Integration and Report Generation
Quantitative data tables, spectra, and statistical outputs are provided, along with a standardized report for subsequent biological interpretation and research validation.
Krasny, L. et al. Molecular Omics, 2021.
Figure 2. Schematic Workflow of the Label-Free DDA-MS and DIA-MS Experiments.
Why Choose MtoZ Biolabs?
✅ High Coverage Acquisition: The DIA mode simultaneously captures all peptide signals, avoiding the omission of low-abundance molecules.
✅ High Reproducibility Quantification: Strong stability across batches, suitable for large-scale comparative analyses.
✅ Unbiased Scanning Mode: No predefined targets are required to discover unknown peptides.
✅ Data Re-Mining Capability: DIA data supports reanalysis based on newly generated spectral libraries.
✅ Customized Workflow Design: Personalized quantitative strategies are provided according to research objectives.
Applications of DIA-Based Peptide Quantification Service
1. Pathway and Network Analysis
Infers key pathway activities through peptide-level changes and supports the construction of regulatory networks.
2. Environmental and Ecological Research
Can be used to detect expression differences of low-abundance peptides in water, soil, or microbial communities, supporting ecological process analysis.
3. Agriculture and Plant Science
Quantifies peptide changes in plant tissues to study stress responses, developmental characteristics, or nutrient regulation mechanisms.
4. Modified Peptide Research Support
Suitable for the quantitative evaluation of post-translational modification–related peptides, such as phosphorylation, glycosylation, or oxidative modifications.
5. Multi-Omics Integration
Can be integrated with transcriptomic or metabolomic data to provide multidimensional support for mechanistic studies.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Peptide Quantification Result Table
4. DIA Quantitative Spectra and Peak Area Plots
5. Bioinformatics Analysis
6. Raw Data Files
FAQ
Q1: What types of samples are suitable?
A1: DIA-based peptide quantification service is suitable for various types of biological samples, including cells, tissues, serum, plasma, and purified proteins. To ensure accurate quantification results, it is recommended that samples maintain high purity, avoid residual salts, surfactants, lipids, or strong buffers, and minimize degradation and external contamination.
Q2: What is the service general workflow?
A2:
Q3: What data formats are provided?
A3: The deliverables include multiple standardized formats to facilitate downstream analysis and archiving:
If special analytical requirements exist, data formats can be customized according to project specifications.
Q4: How should I prepare my samples?
A4: To obtain high-quality DIA quantification data, sample preparation is recommended to follow these requirements:
(1) Purity requirements: avoid high salts, strong buffers, lipids, and precipitates;
(2) Storage conditions: store at -20℃ for short-term and -80℃ for long-term;
(3) Transportation: ship on cold chain or dry ice, ensuring sealed and leak-proof containers;
(4) Additional information: provide sample source, concentration, treatment conditions, and analytical needs to optimize DIA acquisition parameters.
For more information, please refer to Sample Submission Guidelines for Proteomics, Sample Submission Guidelines for Metabolomics.
Start Your Project with MtoZ Biolabs
Contact us to discuss your experimental design or request a quote. Whether you are exploring peptide expression changes under different conditions or investigating their regulatory features in complex systems, MtoZ Biolabs can provide you with high-quality and reproducible DIA quantitative analysis support.
How to order?
