Chitooligosaccharide Analysis Service

Chitooligosaccharides (COS) are a class of oligosaccharides composed of D-glucosamine units linked by β-1,4-glycosidic bonds, typically derived from the partial hydrolysis products of chitin or chitosan. COS possess excellent water solubility and biocompatibility, and exhibit multiple biological activities such as antioxidant, antimicrobial, and immunomodulatory effects. In research, COS are commonly used to explore the relationship between polysaccharide structures and biological functions; in application, they are widely employed in studies of cell proliferation and differentiation, antimicrobial mechanism analysis, and the evaluation of tissue repair material performance.

 



Huang, R H. et al. et al. Carbohydrate Ploymers, 2011.

Figure 1. Schematic Illustration of Chitooligosaccharides (COS) and Their Synthesized Derivatives with Different Substitution Groups.

 

Services at MtoZ Biolabs

Based on high-resolution liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magnetic resonance (NMR) analytical platforms, the chitooligosaccharide analysis service provided by MtoZ Biolabs offers comprehensive qualitative and quantitative detection of chitooligosaccharides. This service accurately analyzes the compositional ratio, degree of polymerization distribution, structural characteristics, and deacetylation degree of chitooligosaccharides, as well as identifies different modification types. Through efficient separation and sensitive detection, the obtained results can be used to evaluate sample purity and structural characteristics, providing scientific evidence for functional mechanism studies, process optimization, and application development of chitooligosaccharides. MtoZ Biolabs provides services including but not limited to the following:

 

1. Quantitative Analysis

Using a high-resolution LC-MS/MS system, this analysis performs high-sensitivity quantitative determination of chitooligosaccharide samples, ensuring data accuracy and reliability.

 

2. Composition Analysis

Evaluates the proportions of different polymerization units and linkage types in chitooligosaccharides, revealing the compositional characteristics of the sample.

 

3. Structural Characterization

Combines mass spectrometry and NMR techniques to elucidate the molecular structure, substitution positions, and deacetylation state of chitooligosaccharides.

 

4. Degree of Polymerization Distribution

Determines the relative content and distribution trend of chitooligosaccharides with different degrees of polymerization, providing a basis for functional differentiation analysis.

 

5. Stability Evaluation

Examines the structural integrity and content variation of chitooligosaccharides under various environmental conditions to assess their stability.

 

6. Metabolic Analysis

Integrates metabolomics approaches to investigate the metabolic transformation and functional mechanisms of chitooligosaccharides in cellular or microbial systems.

 

Sample Submission Suggestions

1. Sample Types

Various types of samples are accepted, including chitin hydrolysates, fermentation broths, microbial culture supernatants, and purified chitooligosaccharide preparations, available in liquid, powder, or lyophilized forms.

Note: Samples should be free from high salt, protein, and organic solvent residues to avoid compromising mass spectrometric sensitivity and result accuracy.

 

2. Sample Storage

Samples should be sealed and protected from light. For short-term storage, keep at -20°C; for long-term storage, it is recommended to store at -80°C. Avoid repeated freeze-thaw cycles to prevent structural degradation.

 

3. Sample Transportation

Liquid samples should be shipped with a dry ice cold chain; lyophilized samples may be sent at room temperature for short durations but must be protected from moisture, high temperatures, and light to ensure chitooligosaccharide stability.

 

Service Advantages

1. Multi-Dimensional Data Output

Covers multiple parameters including content, structure, and degree of polymerization, providing comprehensive analytical results.

 

2. Professional Technical Team

Equipped with extensive experience in carbohydrate chemistry and mass spectrometry analysis, ensuring the accuracy of experimental design and data interpretation.

 

3. Stable and Reliable Results

Employs standardized operating procedures and multiple verification mechanisms to guarantee consistency and reproducibility across different batches.

 

4. Flexible Project Customization

Designs personalized detection and analysis plans based on research objectives to meet diverse experimental requirements.

 

Applications

1. Biological Activity Research

By analyzing the antibacterial, antioxidant, and immunomodulatory properties of chitooligosaccharides, their biological functions and mechanisms of action can be elucidated.

 

2. Metabolic Mechanism Research

Chitooligosaccharide analysis service can be used to investigate the metabolic transformation patterns of chitooligosaccharides in cellular or microbial systems.

 

3. Agricultural Application Research

By analyzing the roles of chitooligosaccharides in plant disease resistance, growth promotion, and signaling regulation, their application potential in agriculture can be evaluated.

 

4. Microecological Function Research

Chitooligosaccharide analysis service can be applied to explore the potential of chitooligosaccharides in regulating gut microbial balance and promoting probiotic activity.

 

FAQ

Q1: Will Impurities or High Salt Content in the Sample Affect the Analysis?

A1: Yes. High salt or impurities may cause ion suppression, reducing signal intensity. MtoZ Biolabs employs desalting and purification steps during sample preparation to eliminate matrix interference and ensure data accuracy.

 

Q2: Can Different Modification Forms of Chitooligosaccharides (Such as Acetylation or Deacetylation) Be Identified?

A2: Yes. By combining high-resolution mass spectrometry fragment ion analysis with reference standards, acetylated, deacetylated, and other substituted forms can be accurately identified, revealing structural differences.

 

Q3: Can Sample Pretreatment Be Performed by the Client?

A3: Yes, but it must follow MtoZ Biolabs’ sample preparation guidelines to ensure analytical compatibility and data reliability.