Butyrylation Proteomics Analysis Service
- Serum/Plasma: 100 µL
- Urine: 2 mL
- Animal Tissue: 200 mg
- Plant Tissue: 10 g
- Microbial Samples: 500 µL
- Protein Extract: 5 mg
- Cells: 1×10⁸ cells
- Lyophilized samples can be shipped at room temperature.
- Solution samples should be refrigerated or shipped with dry ice to prevent freeze-thaw cycles.
- Fresh tissue samples should be transported with dry ice or refrigerated to avoid prolonged exposure to room temperature.
Butyrylation is an emerging post-translational modification (PTM) that plays a critical role in regulating protein structure and function. This modification involves the addition of a butyryl group (C4H9CO-) to lysine residues, significantly impacting cellular processes such as metabolism, gene expression, and disease mechanisms, particularly in cancer and metabolic disorders. Analyzing butyrylation sites and their associated functions is key to understanding disease mechanisms and developing therapeutic strategies.
MtoZ Biolabs offers Butyrylation Proteomics Analysis Service that leverages advanced mass spectrometry platforms combined with bioinformatics analysis to comprehensively identify and quantify butyrylation modification sites in your samples. This service helps uncover the role of butyrylation in various biological contexts and its involvement in cellular metabolism, gene regulation, and disease mechanisms.
Services at MtoZ Biolabs
MtoZ Biolabs’ Butyrylation Proteomics Analysis uses Thermo Fisher’s Q Exactive HF and Orbitrap Fusion mass spectrometry platforms along with advanced Nano-LC technology. These tools enable precise detection and quantification of butyrylation modification sites. Our goal is to identify and quantify butyrylation modifications accurately and explore their roles in cellular metabolism, gene expression, and disease mechanisms.
Additionally, we offer targeted butyrylation analysis for specific proteins, allowing you to focus on the impact of butyrylation modification sites on individual proteins.
Analysis Workflow
We follow a standardized workflow to ensure reliable and reproducible data for Butyrylation Proteomics Analysis. The process includes:
1. Sample Preparation
Proteins are extracted from cells, tissues, or body fluids using buffers containing protease and deacetylase inhibitors to preserve butyrylation modifications.
2. Protein Digestion
Proteins are digested into peptides using trypsin to prepare the sample for mass spectrometry analysis.
3. Butyrylated Peptide Enrichment
Specific anti-butyrylation antibodies are used to efficiently enrich butyrylated peptides, minimizing interference from unmodified peptides.
4. Mass Spectrometry (LC-MS/MS)
Enriched peptides are analyzed using high-resolution mass spectrometry, with data acquisition performed in both DDA and DIA modes.
5. Data Analysis
The mass spectrometry data is processed using specialized software to accurately identify butyrylation modification sites and quantify modification levels.
Figure 1. Butyrylation Proteomics Analysis Workflow
Why Choose MtoZ Biolabs?
✅ Advanced Mass Spectrometry Platforms: Utilizing high-resolution instruments such as Thermo Fisher’s Q Exactive HF and Orbitrap Fusion Lumos, we ensure accurate detection and quantification of butyrylation modifications.
✅ Comprehensive Analytical Coverage: Offering both targeted and global butyrylation profiling to support diverse research objectives across different sample types and experimental models.
✅ High-Quality Data Output: Delivering deep proteome coverage under rigorous quality control, powered by AI-driven bioinformatics for integrated, interpretable, and publication-ready reports.
✅ Tailored Experimental Design: Providing customizable workflows to investigate butyrylation in specific biological systems, pathways, or conditions
✅ One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Sample Submission Suggestions
To ensure efficient and accurate Butyrylation Proteomics Analysis, we recommend the following sample types and quantities:
1. Sample Types & Quantities
2. Transport Conditions
If your sample type is unique or the quantity is low, please contact us to confirm feasibility. MtoZ Biolabs also offers detailed sample submission guidelines upon request.
Applications
Our Butyrylation Proteomics Analysis Service is widely applicable across various research fields, including:
· Epigenetic Regulation: Investigating how butyrylation influences chromatin structure and gene expression.
· Metabolic Signal Transduction: Understanding the role of butyrylation in metabolic enzymes and cellular metabolism.
· Disease Mechanism Research: Exploring the involvement of butyrylation modifications in the onset and progression of diseases such as cancer and neurodegenerative disorders.
· Drug Target Discovery: Leveraging butyrylation as a dynamic PTM in drug target identification.
FAQ
Q1: Can butyrylation coexist with other post-translational modifications?
Yes, butyrylation often coexists with other PTMs, such as acetylation and phosphorylation. These modifications may interact to collectively regulate protein function. We offer joint analysis services to explore these interrelations.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields.
If you're interested in understanding the biological function of butyrylation or need a customized analysis plan, feel free to contact MtoZ Biolabs. We provide accurate and professional Butyrylation Proteomics Analysis Services to support your research progress and product development. Additionally, we offer other PTM services, including phosphorylation, glycosylation, acetylation, and ubiquitination, to help you comprehensively study protein function regulation.
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