α-Amidated Peptides Analysis Service
α-Amidated Peptides Analysis Service is a specialized analytical service for the qualitative and quantitative detection of C-terminal α-amidation in peptides. This service is applicable to peptide function studies, enzymatic mechanism analysis, biomarker discovery, and quality control of peptide-based therapeutics.
Mueller GP. et al. Post-Translational Modification of Proteins. 2019.
C-terminal α-amidation is one of the most common and functionally important post-translational modifications in peptides. It is widely found in neuropeptides, hormones, cytokines, and various bioactive peptides, with over half of all endogenous neuropeptides carrying an α-amide group. This modification is catalyzed by peptidylglycine α-amidating monooxygenase (PAM) and typically involves a C-terminal glycine residue as a precursor, undergoing two sequential steps—oxidation and amidation transfer—to form a stable α-amidated structure. α-Amidation plays a critical role in enhancing peptide receptor affinity, resistance to enzymatic degradation, and overall biological activity. Alterations in this modification have been closely linked to neurological disorders, metabolic abnormalities, and endocrine dysfunction. In the pharmaceutical industry, the α-amide moiety is a key structural feature in the design and modification of many peptide-based therapeutics (such as incretin mimetics), where the integrity of the modification directly affects drug potency and stability.
Leveraging advanced chromatography and mass spectrometry platforms, MtoZ Biolabs offers α-Amidated Peptides Analysis Service to accurately identify the α-amidation modification status of the C-terminus of peptide molecules, locate the modification site, and quantitatively evaluate the modification efficiency, helping customers analyze the regulation mechanism of peptide activity, verify the modification integrity of peptide drugs, and support related functional research and quality control needs.
Analysis Workflow
Mueller GP. et al. Post-Translational Modification of Proteins. 2019.
The analytical workflow of α-Amidated Peptides Analysis Service includes the following key steps:
1. Sample Preparation
Perform necessary pretreatment steps such as desalting, concentration, or enzymatic digestion to remove interfering components and enrich target peptides.
2. Chromatographic Separation
Use reversed-phase liquid chromatography to efficiently separate peptides.
3. Mass Spectrometry Detection
Utilize high-resolution mass spectrometry to acquire full-scan and fragmentation data of peptides, detecting mass shifts and diagnostic ions associated with α-amidation.
4. Data Analysis
Interpret mass spectrometry data using specialized software to precisely localize α-amidation sites and quantify modification levels.
5. Result Reporting
Deliver a comprehensive report including modification site identification, abundance analysis, and annotated MS/MS spectra.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced α-Amidated Peptides Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all α-Amidated Peptides Analysis Service data, providing clients with a comprehensive data report.
4. Multi-Sample Compatibility: Compatible with peptide solutions, crude extracts, and synthetic products, offering flexibility for diverse research contexts.
Sample Submission Suggestions
It is recommended to submit samples as lyophilized powder or stored at low temperature (–80°C). For liquid samples, ship on dry ice and avoid repeated freeze–thaw cycles.
If your samples have special conditions (e.g., low-abundance targets, limited sample amount), please contact the MtoZ Biolabs technical team in advance. We offer customized sample preparation advice and pretreatment support to ensure stable and reliable modification analysis results.
Applications
Application Examples of the α-Amidated Peptides Analysis Service:
Activity Validation of Neuropeptides
Determine whether peptides are fully C-terminally α-amidated and in their mature functional state.
PAM Enzyme Function Studies and Activity Screening
Analyze amidation efficiency under various conditions to screen for PAM inhibitors or activators.
Modification Mechanism and Signaling Pathway Studies
Use modification data to explore peptide involvement in cellular signaling and modification dependency.
Quality Control and Consistency Evaluation of Peptide Drugs
Assess modification integrity of synthetic peptides for drug development and manufacturing.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
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