Allolactose Analysis Service
- Utilizes the anionic behavior of sugars in strong alkaline conditions to achieve precise separation.
- Enables direct electrochemical detection of allolactose based on its reducing properties.
- Provides high selectivity, reproducibility, and sensitivity without complex derivatization steps.
- Combines efficient chromatographic separation with the molecular specificity of mass spectrometry.
- Delivers rapid and accurate quantification of allolactose in complex matrices with minimal interference.
- Offers superior sensitivity, excellent specificity, and a wide dynamic detection range.
- Employs β-galactosidase to hydrolyze allolactose into glucose and galactose.
- Quantifies hydrolysis products indirectly through colorimetric or fluorometric assays.
- Provides a cost-effective and reliable solution for routine detection in biological or fermentation samples.
- Uses enzyme-linked electrochemical biosensors for real-time monitoring of allolactose levels.
- Suitable for dynamic process control in fermentation and enzymatic reaction studies.
- Offers rapid, high-throughput measurement with excellent specificity and operational simplicity.
MtoZ Biolabs provides comprehensive Allolactose Analysis Services based on advanced chromatography, mass spectrometry, enzymatic assays, and biosensor technologies. Our Allolactose Analysis Services enable accurate quantification, purity assessment, structural characterization, stability testing, and metabolic evaluation of allolactose in biological and industrial samples. This service supports studies in microbiology, metabolic regulation, and biotechnology with reliable, high-resolution analytical data.
What Is Allolactose?
Allolactose is a β-1,6-linked disaccharide composed of galactose and glucose. It is a structural isomer of lactose, formed through transgalactosylation reactions catalyzed by β-galactosidase. Allolactose plays a critical biological role as the natural inducer of the lac operon in Escherichia coli and related bacteria, regulating genes responsible for lactose metabolism.
Source: Wikipedia
Figure 1. The Structure of Allolactose
The biological importance of allolactose lies in its dual roles in gene regulation and metabolic activity:
1. Lac Operon Inducer
Allolactose acts as the natural inducer of the lac operon. It binds to the lac repressor protein, releasing its inhibition and allowing RNA polymerase to activate the transcription of structural genes involved in lactose metabolism.
2. Metabolic Regulation
The presence of allolactose reflects active lactose catabolism and helps researchers evaluate β-galactosidase activity and enzyme kinetics in microbial systems.
3. Human Gut Function
Although not digested by human enzymes, allolactose reaches the colon intact, where it is fermented by intestinal bacteria to produce short-chain fatty acids (SCFAs). These SCFAs provide energy to colonocytes and contribute to gut health.
4. Microbiota Balance
Allolactose helps maintain the balance of intestinal microflora by promoting beneficial bacterial growth and reducing harmful compounds like ammonia and sulfides.
In summary, allolactose is not only essential for understanding gene regulation and enzymatic activity in microorganisms but also plays a potential role in human intestinal health and metabolic research.
Service at MtoZ Biolabs
MtoZ Biolabs provides a full range of Allolactose Analysis Services designed to meet research and industrial needs through advanced chromatographic, spectrometric, enzymatic, and biosensor-based methods. Each approach offers unique advantages in terms of accuracy, sensitivity, and detection efficiency.
1. High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD)
2. Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
3. Enzymatic Method
4. Biosensor-Based Detection
Service Advantages
✔️Comprehensive Analytical Solutions integrating chromatography, mass spectrometry, and enzymatic platforms.
✔️High Specificity and Sensitivity for distinguishing allolactose from structural isomers.
✔️Customizable Services tailored to project goals, sample type, and detection requirements.
✔️Reliable and Reproducible Results supported by strict quality control and instrument calibration.
✔️Expert Data Interpretation provided by experienced carbohydrate chemists and bioanalysts.
✔️Efficient Turnaround Time with flexible scheduling and rapid reporting.
Applications
1. Microbial Genetics and Physiology: Studying the role of allolactose as a natural inducer in lac operon regulation.
2. Enzymology and Biocatalysis: Evaluating β-galactosidase activity and its transgalactosylation efficiency.
3. Biotechnology and Fermentation: Monitoring enzymatic conversion of lactose and optimizing fermentation parameters.
4. Food and Dairy Industry: Investigating lactose transformation products and functional disaccharide formation.
5. Pharmaceutical Research: Supporting metabolic studies and formulation of enzyme-based therapeutics.
Sample Submission Suggestions
1. Sample Types
Food products, beverages, pharmaceuticals, fermentation broths, plant extracts, or other biological samples.
2. Recommended Sample Quantity
Solid samples: ≥ 2 g
Liquid samples: ≥ 2 mL
3. Storage and Transport
Samples should be sealed in airtight containers and stored at –80°C. Transport on dry ice or with ice packs to prevent degradation.
*Note: For samples with limited availability or specific handling requirements, please contact MtoZ Biolabs for customized sample preparation instructions.
Deliverables
1. Detailed experimental information including instrumentation, analytical methods, and parameters
2. Raw and processed data with chromatograms, spectra, and calibration curves
3. Comprehensive quality control evaluation ensuring data reliability and reproducibility
4. Bioinformatics and statistical analysis for data interpretation and metabolic insights
5. Customized analytical report summarizing results, conclusions, and project-specific recommendations
Our Allolactose Analysis Service is designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.
How to order?
