Acetylomics Data Analysis Service
Protein acetylation is a widely occurring and functionally diverse type of post-translational modification (PTM), primarily including N-terminal acetylation and ε-acetylation on lysine residues. By regulating protein stability, subcellular localization, molecular interactions, and gene expression, acetylation plays a critical role in numerous cellular processes. In recent years, advances in mass spectrometry have enabled acetylomics to emerge as a powerful strategy for studying disease mechanisms, metabolic pathways, and drug target validation.
Zhong, Q. et al. MedComm (2020). 2023.
Figure 1. Representative Functions of Protein Acetylation
Based on high-resolution mass spectrometry platforms and robust data analysis capabilities, MtoZ Biolabs offers a professional acetylomics data analysis service. This service includes comprehensive modules such as acetylation site identification, quantitative analysis, functional annotation, and pathway enrichment—designed to help clients systematically explore the regulatory roles and biological implications of protein acetylation. The service is suitable for both fundamental and translational research.
Services at MtoZ Biolabs
MtoZ Biolabs' acetylomics analysis integrates multiple mainstream protein databases (e.g., UniProt, GO, KEGG) and computational tools, enabling multi-level interpretation from the protein to the systems biology scale. Our service supports both isobaric labeling methods (TMT, iTRAQ) and label-free quantitative strategies.
MtoZ Biolabs offers professional acetylomics data analysis services to help clients deeply explore acetylation modifications from existing mass spectrometry datasets. If the quality or results of your current data are unsatisfactory, we also provide an integrated Acetylproteomics Analysis Service—from experimental detection to data interpretation. Leveraging high-resolution mass spectrometry platforms, we ensure accurate identification and quantification of acetylation sites, along with comprehensive functional annotation and detailed result reporting to meet diverse research needs.
Analysis Workflow
1. Raw Data Preprocessing
Includes file format conversion, peak detection, quality control, and background noise filtering to ensure reliable analysis.
2. Acetylation Site Identification
Utilizes database search algorithms to identify acetylated peptides and modification sites, with localization scores used to evaluate site confidence.
3. Quantitative Expression Analysis
Measures expression differences in acetylation levels across experimental groups using tag intensities or peptide peak areas.
4. Functional Annotation and Classification
Encompasses GO enrichment, subcellular localization prediction, and Pfam domain analysis.
5. Pathway and Network Analysis
Performs pathway enrichment using KEGG and Reactome databases, providing insights into regulatory networks involving acetylated proteins.
6. Visualization and Report Delivery
Delivers comprehensive graphical outputs (e.g., heatmaps, volcano plots, enrichment bubble charts) and standardized reports suitable for publication or internal review.
Service Advantages
1. Advanced Analytical Platform
MtoZ Biolabs operates multiple high-resolution and high-sensitivity mass spectrometers, including Thermo Scientific Orbitrap Fusion Lumos and Q Exactive HF systems. These instruments offer exceptional mass accuracy, scan speed, and dynamic range, enabling confident identification of low-abundance acetylated peptides. Our nano-LC systems and automated sample loading procedures enhance reproducibility and throughput—ideal for complex or large-scale studies.
2. Standardized Workflow, High-Quality Output
All data is processed by experienced analysts with years of proteomics and PTM expertise. The pipeline includes rigorous quality control checkpoints (e.g., signal intensity distribution, peptide ID rates, site localization probability), ensuring consistent and reproducible results. Cross-batch comparison is also supported for multi-group or longitudinal studies.
3. Strong Compatibility with Multiple Data Types
Supports various data formats and quantitation strategies, including Label-Free, TMT, and iTRAQ. Customized preprocessing workflows and parameter optimization are available—for example, isotope correction in TMT data or normalization and imputation strategies for label-free datasets. Accepted input formats include RAW, mzML, and mzXML.
4. Comprehensive Annotation
Results include not only acetylation site information but also extensive functional annotation: GO categories (MF, BP, CC), Pfam domain mapping, UniProt features, and predicted protein–protein interaction networks. Pathway enrichment analyses via KEGG and Reactome help reveal functional roles and regulatory pathways associated with acetylated proteins.
5. Professional Technical Support
We offer end-to-end support throughout the project lifecycle. Pre-analysis consultation includes assistance with experimental design, sample prep strategy, and enrichment method selection. During data analysis, we tailor reporting depth and format to client needs. Post-delivery, we offer result interpretation via written reports or remote meetings. For publication purposes, we assist in figure preparation, methods section writing, and reviewer response strategies.
Applications
1. Fundamental Biological Research
Explore the regulatory roles of acetylation in cell cycle control, DNA repair, chromatin remodeling, and more.
2. Disease Mechanism Studies
Investigate aberrant acetylation patterns in cancer, neurodegenerative diseases, and inflammatory conditions.
3. Drug Mechanism Evaluation
Assess the impact of drug candidates on acetylation status of target proteins, aiding in mechanism-of-action studies and target validation.
4. Microbial Stress Response Research
Analyze dynamic changes in acetylation levels in response to environmental stress (e.g., nutrient shifts, pH, temperature) in bacteria or yeast.
Protein acetylation is a key regulatory mechanism in epigenetics and signal transduction. Global analysis of acetylation patterns provides valuable insights into the molecular mechanisms underlying health and disease. With advanced mass spectrometry platforms and an experienced bioinformatics team, MtoZ Biolabs offers high-quality, customizable acetylomics data analysis services. If you have relevant research needs, please feel free to contact us for detailed consultation and service planning.
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