4-Hydroxynonenal Proteomics Analysis Service

4-Hydroxynonenal Proteomics Analysis Service is a service that utilizes Mass Spectrometry (MS) and related enrichment techniques to analyze proteins modified by 4-hydroxynonenal (4-HNE) and their modification sites. By precisely capturing and identifying 4-HNE-modified peptides, this service helps researchers uncover the distribution, functional changes, and disease associations of 4-HNE-modified proteins. It provides crucial data support for the study, early diagnosis, and development of therapeutic strategies related to oxidative stress-associated diseases.

Rauniyar N. et al. Anal Bioanal Chem. 2007.

4-HNE is a common lipid peroxidation product with an α,β-unsaturated ketone structure, typically generated through oxidative stress in cells, and is associated with various diseases, such as neurodegenerative diseases, cancer, and cardiovascular diseases. It can react with amino acid residues on proteins (such as lysine, cysteine, and histidine) to form modified products, which in turn affect protein function, stability, and cellular biological processes. Therefore, detecting and analyzing 4-Hydroxynonenal-modified proteins is crucial for the study of oxidative stress, disease mechanisms, and drug development.

Based on advanced chromatography and mass spectrometry technologies, MtoZ Biolabs offers 4-Hydroxynonenal Proteomics Analysis Service to identify and quantitatively analyze 4-HNE-modified proteins, helping researchers deeply explore the biological effects triggered by oxidative stress, uncover potential disease mechanisms, and provide data support for drug development and biomarker discovery.

Analysis Workflow

The general process of 4-Hydroxynonenal Proteomics Analysis Service is as follows:

1. Sample Preparation and Protein Extraction

Extract total proteins from the sample and perform pre-treatment.

2. Enrichment of 4-HNE-modified Proteins

Use specific 4-HNE-modified antibodies or chemical probes to enrich the modified proteins, ensuring that the modified peptides are captured.

3. Proteolysis

Digest the enriched proteins with enzymes to break them down into peptides that can be analyzed.

4. Liquid Chromatography-Mass Spectrometry (LC-MS/MS) Analysis

Use a liquid chromatography system to separate the peptides, and perform high-resolution analysis with a mass spectrometer to obtain peptide mass and sequence information.

5. Data Processing and Analysis

Analyze the mass spectrometry data using specialized software to identify 4-HNE modification sites and perform functional annotation.

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced 4-Hydroxynonenal Proteomics Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all 4-Hydroxynonenal Proteomics Analysis data, providing clients with a comprehensive data report.

Sample Submission Suggestions

Sample Type: Cells, tissues, blood, urine, and other samples.

Shipping Requirements: Dry ice is recommended for shipping.

Additional Notes: If you are unsure about sample preparation and submission, feel free to contact our technical support team for assistance.

Applications

Domenico FD. et al. Free Radical Bio Med. 2017.

Application examples of 4-Hydroxynonenal Proteomics Analysis Service:

Oxidative Stress Research

Through analysis of 4-HNE-modified proteins, this service helps reveal the role of oxidative stress in cellular damage, aging, neurodegenerative diseases, and more.

Disease Mechanism Research

4-HNE modification is associated with many diseases. By analyzing the 4-HNE modifications on related proteins, this service aids in understanding the molecular mechanisms of disease development.

Biomarker Discovery

Identifying specific proteins modified by 4-HNE as biomarkers contributes to early disease diagnosis and risk assessment.

Drug Development

Analyzing 4-HNE-modified proteins provides valuable data for the development of drugs targeting oxidative stress and regulating 4-HNE modifications.