Which Instruments Can Visualize Fluorescent Proteins on Native PAGE Without Staining? What Are the Common Markers
Native PAGE (polyacrylamide gel electrophoresis) is a technique for protein separation that preserves the native structure of proteins, as it does not involve denaturation or staining. In this method, fluorescent proteins can be directly visualized using fluorescence imaging systems. Additionally, several specific proteins are commonly employed as markers for Native PAGE.
Instruments Used
Fluorescent proteins in Native PAGE can be directly visualized using fluorescence imaging systems. These systems excite the proteins at specific wavelengths and capture the emitted fluorescence, enabling detection without the need for staining. This approach is particularly valuable for preserving protein functionality and native conformation during analysis.
Common Native PAGE Markers
1. Bovine Serum Albumin (BSA)
Bovine serum albumin is frequently used as a Native PAGE marker to assess protein migration rates and evaluate the performance of the electrophoresis system.
2. Blue Native Marker
The Blue Native marker is a mixture containing fluorescent proteins and is utilized as a molecular weight reference in Native PAGE.
3. Cytochrome C
Cytochrome C is a well-characterized marker protein with well-documented migration behavior and electrophoretic properties under native conditions.
4. Lactate Dehydrogenase (LDH)
Lactate dehydrogenase is another widely used Native PAGE marker, often employed to assess electrophoresis conditions and gel resolution.
5. Hexokinase
Hexokinase serves as an additional example of a marker in Native PAGE, particularly useful for referencing proteins with relatively low molecular weights.
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