What Is the Blue Liquid Added to Protein Samples During Electrophoresis
In protein electrophoresis experiments, samples are typically prepared using a solution known as protein loading buffer. This buffer is an aqueous mixture that contains denaturing agents, reducing agents, and tracking dyes. The denaturing and reducing agents serve to unfold the protein structures and break disulfide bonds, thereby linearizing the proteins to promote consistent migration through the gel matrix during electrophoresis. The tracking dye is included to provide visual monitoring of the electrophoresis progress. Commonly used dyes include bromophenol blue and methylene green, which generate a visible blue front during electrophoresis, enabling real-time observation of the separation process.
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