Two-Dimensional Gel Electrophoresis Service

Two-dimensional gel electrophoresis (2-DE) is a powerful and widely used method. It combines IEF and SDS-PAGE. First, proteins are separated by their isoelectric point (pI) in IEF, and then by SDS-PAGE based on molecular weight. Each protein spot on the two-dimensional gel can be eluted and identified by high-throughput mass spectrometry. Therefore, 2-DE is particularly suitable for comparing protein profiles between different tissues, different conditions, or between control and experimental samples.

 

Analysis Workflow 

MtoZ Biolabs provides 2-DE services. The 2-DE service can separate thousands of proteins from complex mixtures for subsequent quantitative analysis.

 

1. Sample Preparation

The preparation process depends on the sample type.

 

2. First Separation by IEF

Load the protein mixture onto a pH gradient gel, and proteins migrate under the influence of an electric field.

 

3. Second Separation by SDS-PAGE

This method generally includes three steps: preparing the gel, equilibrating the pH gradient strips fixed in SDS buffer, and conducting electrophoresis.

 

4. Staining

Proteins are stained using various staining methods, such as silver staining and Coomassie Brilliant Blue staining.

 

5. Result Analysis

Besides protein separation, protein molecular weight, pI, and content can also be determined.

 

Sample Submission Requirements 

We accept various types of samples, including but not limited to the following types: 

1. Purified Protein 

2. Frozen Cell Pellets or Tissue 

3. Cells (such as Blood Cells) or Tissue 

4. Serum/Plasma/Urine 

5. Cell Cultures

6. Fermentation Cultures

7. Microorganisms 

8. Plants 

9. Others

 

Service Advantages 

1. High Throughput

Analyze thousands of proteins with just one 2-DE. 

 

2. High Resolution

Proteins are resolved by pI and molecular weight, and protein characterization is achieved through post-translational modifications that affect their charge state. 

 

3. Advanced Bioinformatics Software

We have SameSpots, Delta2D, ImageMaster, and other software for detecting and quantifying protein spots. 

 

4. Low Cost

2-DE is relatively cheaper compared to mass spectrometry analysis. 

 

5. Flexibility

MtoZ Biolabs provides customizable analytical services and designs optimal experimental plans to achieve your research goals within your budget.

 

Applications 

1. Protein Profiling 

2. Protein Isoform Distribution Analysis 

3. Protein Quantification 

4. Post-Translational Modifications of Proteins 

5. Protein and Antibody Quality Control 

6. Host Cell Protein Analysis 

7. Downstream Analyses Including Western Blotting 

8. Developmental Genetics, Disease Etiology, Biomarker, and Target Discovery

 

With many years of experience, MtoZ Biolabs provides high-resolution and high-throughput 2-DE services for protein analysis. 2-DE analysis services can directly observe changes in proteins and post-translational modifications, as well as discover and detect biomarkers and new drugs. We can also provide customizable services according to your project requirements.