What Analytical Methods Are Available for Metabolomic Profiling of Morinda officinalis Polysaccharides?
Currently, metabolomic studies of Morinda officinalis polysaccharides primarily utilize high-performance liquid chromatography–mass spectrometry (HPLC-MS) and gas chromatography–mass spectrometry (GC-MS), depending on the nature of the sample and the target analytes.
Liquid Chromatography–Mass Spectrometry (LC-MS)
1. Principle
Metabolites are first separated via liquid chromatography and subsequently ionized and analyzed by mass spectrometry.
2. Advantages
High sensitivity and resolution; well-suited for the analysis of complex biological matrices.
3. Application
Applicable to a broad range of metabolite classes, including small molecules and oligosaccharides derived from polysaccharides.
Gas Chromatography–Mass Spectrometry (GC-MS)
1. Principle
After chemical derivatization to enhance volatility and thermal stability, metabolites are separated by gas chromatography and detected using mass spectrometry.
2. Advantages
High sensitivity; particularly effective for the detection of volatile and semi-volatile compounds.
3. Application
Suitable for analyzing derivatized monosaccharide or oligosaccharide fragments generated from acid- or enzymatic hydrolysis of polysaccharides.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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