Ultra-deep and Quantitative Saliva Proteomics Solutions
- DIA-MS for comprehensive protein identification and quantification, suitable for exploratory studies.
- PRM and other targeted proteomics techniques for high-precision quantification of specific proteins, ideal for validation studies.
- Early biomarker discovery for oral cancer, head and neck cancer, and Alzheimer’s disease.
- Molecular classification and treatment monitoring for inflammatory diseases (e.g., periodontitis).
- Investigating saliva-microbiome interactions.
- Pharmacokinetics/pharmacodynamics (PK/PD) studies.
- Saliva proteomics for dynamic health monitoring.
- Evaluating the effects of sports medicine and nutritional interventions.
Saliva proteomics is a field that utilizes high-throughput proteomic analysis technologies to study the composition and dynamic changes of proteins in saliva. Compared to blood and urine, saliva collection is non-invasive, convenient, and reflective of an individual’s physiological state, making it a promising tool for early disease diagnosis, biomarker discovery, and personalized medicine. However, saliva proteomes exhibit high complexity and variability, with low-abundance proteins being difficult to detect, posing a major challenge in high-sensitivity and ultra-deep quantitative analysis. Quantitative saliva proteomics enables the precise measurement of protein expression changes in saliva, providing valuable molecular insights for biomarker identification and precision medicine applications.
To address these challenges, MtoZ Biolabs offers Ultra-deep and Quantitative Saliva Proteomics Solutions that leverages advanced mass spectrometry platforms and integrating data-independent acquisition (DIA) and targeted proteomics. Ultra-deep and Quantitative Saliva Proteomics Solutions enables comprehensive coverage and precise quantification of the saliva proteome, supporting both fundamental research and clinical applications. With customized analytical strategies, MtoZ Biolabs provides robust solutions for disease mechanism studies, drug discovery, and biomarker validation.
Services at MtoZ Biolabs
The Ultra-deep and Quantitative Saliva Proteomics Solutions at MtoZ Biolabs employs DIA-MS and targeted proteomics to offer a comprehensive workflow from protein identification to quantitative analysis. Our services include:
· DIA-MS for High-Throughput Quantitative Analysis
Utilizing high-resolution mass spectrometry for unbiased, high-coverage proteomic analysis, enabling precise quantification in complex proteomes.
· Targeted Proteomics
Implementing parallel reaction monitoring (PRM) for high-sensitivity, precise quantification of specific proteins, making it ideal for biomarker validation.
· High-Resolution Quantification Analysis
Employing TMT or iTRAQ labeling strategies to enhance experimental reproducibility, facilitating large-scale proteomic studies.
Analysis Workflow
Ultra-deep and Quantitative Saliva Proteomics Solutions ensures comprehensive protein profiling and precise quantification through an optimized workflow integrating advanced sample processing, high-resolution mass spectrometry, and robust bioinformatics analysis.
1. Sample Processing
Quality assessment, protein extraction, and high-abundance protein depletion to enhance low-abundance protein detection.
2. Protein Digestion
Using optimized enzymatic digestion strategies to improve peptide recovery and reproducibility, ensuring quantitative accuracy.
3. Mass Spectrometry Detection
4. Data Analysis
Constructing high-resolution proteomic datasets, performing quantification, differential expression analysis, and functional annotation, and integrating machine learning algorithms for data optimization.
Grassl, N. et al. Genome Med. 2016.
Figure 1. Workflow for Ultra-Deep and Quantitative Saliva Proteomics
Why Choose MtoZ Biolabs?
1. Ultra-Deep Coverage: DIA technology enables detection of more low-abundance proteins in a single experiment, improving the identification of key biomarkers.
2. High-Precision Quantification: PRM and targeted proteomics provide high-sensitivity, accurate quantification for biomarker validation and mechanistic studies.
3. Flexible Experimental Strategies: Supporting DIA, PRM, and TMT/iTRAQ for customized quantitative proteomics approaches, maximizing data utility.
4. State-of-the-Art Mass Spectrometry Platforms: Leveraging high-resolution Orbitrap mass spectrometry and nano-LC to ensure high sensitivity and data accuracy.
5. Comprehensive Bioinformatics Support: Integrating multi-omics data to provide protein interaction networks, biomarker discovery, and functional enrichment analysis.
Applications
· Disease Diagnosis & Prognostics
· Mechanistic Research & Drug Development
· Personalized Medicine & Health Monitoring
Case Study
Case 1
A 2024 study utilized DIA-MS for comprehensive proteomic profiling of whole saliva and cell-free saliva samples. The study revealed significant differences in protein expression, enhancing our understanding of salivary components in biology and disease diagnosis. The DIA approach enabled the accurate quantification and identification of numerous proteins, supporting biomarker discovery and in-depth saliva proteomics research.
Jiao, L. L. et al. Proteomics Clin Appl. 2024.
Case 2
A groundbreaking study combined discovery and targeted proteomics to identify prognostic markers in OSCC patients. Using SRM-MS, researchers analyzed key proteins in saliva samples, such as LTA4H, COL6A1, and CSTB, which differentiated patients with and without lymph node metastasis. This study highlights the potential of saliva proteomics in clinical prognosis and validates Ultra-deep and Quantitative Saliva Proteomics Solutions for high-level proteomics research.
Carnielli, C. M. et al. Nat. Commun. 2018.
What Could be Included in the Report?
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment
4. Data Analysis, Preprocessing, and Estimation
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs' Ultra-deep and Quantitative Saliva Proteomics Solutions offers cutting-edge DIA-MS and targeted proteomics techniques, providing comprehensive saliva proteome analysis for biomarker discovery, disease research, and precision medicine.
Contact us today for customized research solutions!
How to order?
