Targeted SUMOylation Analysis Service

MtoZ Biolabs provides Targeted SUMOylation Analysis Service focused on comprehensive profiling of SUMO modifications on specific proteins of interest supplied by clients. This service enables systematic identification, quantification, and structural characterization of SUMO-conjugated proteins to reveal how SUMOylation regulates protein function, stability, and cellular localization. By integrating advanced LC-MS/MS technology with optimized SUMO-remnant enrichment workflows, MtoZ Biolabs delivers high-quality, reproducible, and site-specific SUMOylation data to support molecular biology and therapeutic research.

Overview

SUMOylation is a reversible post-translational modification in which Small Ubiquitin-like Modifier (SUMO) proteins are covalently attached to lysine residues on target substrates via an enzymatic cascade involving E1 activating, E2 conjugating, and E3 ligating enzymes. This modification does not typically mark proteins for degradation but instead modulates their localization, activity, and interaction networks. SUMOylation is involved in vital processes such as transcriptional regulation, DNA repair, chromatin organization, and stress response, and its dysregulation has been linked to cancer, neurodegeneration, and immune disorders.

Comprehensive analysis of SUMOylation on targeted proteins provides valuable insight into protein function and regulatory mechanisms. For example, SUMOylation of p53 alters its transcriptional activity, HDACs undergo SUMO modification to regulate gene silencing, and PCNA SUMOylation plays a role in DNA damage repair and replication control. MtoZ Biolabs performs high-resolution SUMOylation profiling on client-defined proteins to support studies of signaling, regulation, and therapeutic discovery.

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Li, M. et al. Nat. Commun. 2018.

Figure 1. Model of SUMO2-PCNA-Mediated Resolution of Transcription-Replication Conflicts

Targeted SUMOylation Analysis Service at MtoZ Biolabs

✅ SUMOylated Peptide Enrichment

SUMO-modified peptides are selectively enriched using affinity purification, enabling high specificity and reproducibility in downstream mass spectrometric analysis.

✅ Targeted SUMOylation Identification

High-resolution LC-MS/MS on Thermo Fisher Orbitrap Fusion Lumos and Q Exactive HF systems enables accurate site localization and mapping of SUMO modification patterns, including mono- and poly-SUMO conjugation.

✅ Targeted SUMOylation Quantification

Quantitative LC-MS/MS approaches, such as label-free, SILAC, and TMT labeling, are used to measure SUMOylation changes across different conditions, ensuring precise assessment of modification dynamics.

✅ Targeted SUMOylation Structural Characterization

Advanced LC-MS/MS, combined with top-down or crosslinking MS, provides insights into structural alterations and interaction shifts induced by SUMO conjugation.

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform: MtoZ Biolabs established advanced Targeted protein PTM analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. Optimized Enrichment Method: SUMO-remnant affinity purification ensures selective capture of modified peptides and consistent data quality.

3. Comprehensive Quantitative Capabilities: Multiple quantification methods, including SILAC and TMT labeling, allow accurate evaluation of SUMOylation dynamics in diverse biological contexts.

4. Expert Scientific Team: Experienced proteomics specialists provide detailed analytical reports, including site identification, quantitative results, and biological interpretation tailored to research goals.

5. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

Sample Submission Guidelines

Proper sample preparation is essential for accurate SUMOylation analysis. MtoZ Biolabs accepts a wide range of biological materials, including purified proteins, cell lysates, and tissue extracts.

To ensure data reliability, please follow these recommendations before submission:

Protein Concentration and Purity: Ensure sufficient purity and concentration for digestion and enrichment. Avoid detergents, chaotropes, and strong reducing agents.

Sample Storage: Store samples at –80°C immediately after preparation to prevent degradation.

Buffer Requirements: Use MS-compatible or volatile buffers such as ammonium bicarbonate and indicate all additives used.

Typical sample requirements are listed below for reference:

Sample Type	Recommended Amount
Purified proteins	≥100 µg
Cell lysates or tissue extracts	≥500 µL
Affinity-purified complexes	≥50 µg

For more information, please refer to Sample Submission Guidelines for Proteomics.

Applications of Targeted SUMOylation Analysis Service

Targeted SUMOylation analysis supports a broad range of molecular and biomedical studies, including:

Regulation of transcription and chromatin architecture

DNA replication and repair mechanisms

Protein transport and localization

Cellular stress and senescence pathways

Oncology, neurology, and immunology research

Start Your Project with MtoZ Biolabs

MtoZ Biolabs offers high-precision SUMOylation analysis for targeted proteins, providing reliable, site-level insight into modification patterns and functional regulation.

Contact us today to discuss your project or request a customized quotation. Our scientific team will design an analytical solution tailored to your research objectives.