Targeted Metabolites Profiling Service

    MtoZ Biolabs provides a Targeted Metabolites Profiling Service that delivers high accuracy and reproducibility for the quantification of specific metabolites across diverse biological samples. The Targeted Metabolites Profiling Service focuses on a predefined set of metabolites of interest, enabling researchers to obtain precise and sensitive measurements that are directly aligned with specific biological questions or experimental hypotheses. By applying advanced mass spectrometry platforms, targeted metabolomics can selectively detect and quantify specific metabolites within complex mixtures. Moreover, the results can be integrated with other experimental approaches, such as transcriptomics, proteomics, or functional assays, to provide deeper insights into molecular mechanisms and clarify the biological roles of metabolites in health, disease, and treatment responses.

    Service at MtoZ Biolabs

    At MtoZ Biolabs, targeted metabolomics profiling is carried out through a systematic integration of chromatographic separation and mass spectrometry. Gas chromatography–mass spectrometry (GC-MS) is applied for volatile and thermally stable metabolites, while liquid chromatography–mass spectrometry (LC-MS) is employed for polar and thermolabile compounds. Together, these complementary techniques ensure broad chemical coverage and high analytical resolution. Quantification is achieved using selected ion monitoring (SIM) and multiple reaction monitoring (MRM). SIM enables sensitive tracking of specific ions, while MRM provides highly selective monitoring of precursor-product ion transitions, minimizing background interference. This dual strategy ensures that even low-abundance metabolites can be detected and quantified with confidence.

    Our Targeted Metabolites Profiling Service covers a wide range of metabolite classes, including but not limited to:

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    Analysis Workflow

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    Service Advantages

    ✔️Expert team with extensive experience in targeted metabolomics

    ✔️State-of-the-art mass spectrometry platforms for reliable data

    ✔️Comprehensive bioinformatics support for pathway-level insights

    ✔️Transparent project management and timely delivery

    ✔️Flexible solutions designed to meet both academic and industrial requirements

    Applications

    • Validation of hypotheses generated from untargeted metabolomics studies
    • Confirmation of biomarker candidates identified in discovery experiments
    • Quantitative verification of metabolites highlighted in pathway enrichment analyses
    • Verification of metabolic signatures associated with specific interventions or treatments

    Sample Submission Suggestions

    1. Sample Types

    Microbial cultures, cell cultures, animal tissues, plant tissues, animal biofluids, and other biological samples.

    2. Storage and Transport

    Samples should be snap-frozen and stored at –80°C, shipped on dry ice, and protected from repeated freeze–thaw cycles.

    3. Notes

    Avoid detergents, salts, preservatives, or other additives that may interfere with mass spectrometry detection.

    Clients are encouraged to contact MtoZ Biolabs before sample preparation to receive tailored instructions for their specific project.

    Deliverables

    • Detailed experimental information, including methods and conditions
    • Raw data files
    • Spectral and chromatographic outputs
    • Data preprocessing and analysis results
    • Bioinformatics analysis
    • A tailored report based on project needs

    For more information about our Targeted Metabolites Profiling Service, please contact us. Our technical specialists are available to provide a free business assessment.

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