Single Reaction Monitoring Analysis Service

The Single Reaction Monitoring Analysis Service  utilizes cutting-edge mass spectrometry technology to achieve efficient quantitative analysis of low-abundance proteins in complex biological samples. Single Reaction Monitoring (SRM) technique quantifies by selecting and monitoring preset peptides and their characteristic ions, significantly enhancing detection sensitivity and specificity, particularly for low-abundance proteins. Compared to traditional full-scan mass spectrometry methods, Single Reaction Monitoring reduces background interference from other proteins in the sample by focusing only on ions related to the target peptides, thereby providing more precise and reliable data.

 



Guerrero L, et al. Metabolites. 2022.

Principle of Single Reaction Monitoring Analysis 

 

Services at MtoZ Biolabs

MtoZ Biolabs’ Single Reaction Monitoring Analysis Service offers high sensitivity and specificity in protein quantification, with a focus on accurate monitoring of target peptides. By leveraging advanced mass spectrometry technology and stringent quality control, we ensure precise detection of low-abundance proteins in complex biological samples. Through customized analysis plans, MtoZ Biolabs provides highly reliable experimental data, widely applied in areas such as disease biomarker screening and drug target validation. Throughout the analysis, we eliminate background interference, ensuring high reproducibility and scientific integrity of the data, helping clients gain a deeper understanding of protein function and supporting precise decision-making in both research and clinical applications.

 

Analysis Workflow

MtoZ Biolabs' Single Reaction Monitoring Analysis Service is managed by our experienced research scientists and technical team throughout the entire process, from sample receipt to final data report, ensuring the accuracy of each step and the reliability of the data.

1. Sample Preparation and Processing

Based on the sample type provided by the client, MtoZ Biolabs' team carries out detailed sample preprocessing, including protein extraction, peptide separation, concentration, and other steps. This ensures a solid foundation for the subsequent Single Reaction Monitoring analysis.

 

2. Mass Spectrometry Analysis and Data Collection

During the Single Reaction Monitoring phase, we configure appropriate analysis parameters based on the peptide sequence and mass spectrometry characteristics of the target proteins to perform mass spectrometry scanning. Our mass spectrometers can simultaneously monitor multiple target peptides, ensuring the accuracy of high-throughput data collection.

 

3. Data Analysis and Quantitative Reporting

Using advanced data analysis software, we extract clear quantitative results from complex data, providing detailed reports to help clients understand the analysis outcomes and offering scientific evidence for further research or decision-making.

 

4. Customized Solutions

MtoZ Biolabs offers flexible, customized services based on different client needs, including quantification of specific proteins, protein variation studies in disease models, target validation, and more. We ensure that each project receives the most suitable analysis plan and data support.

 



 

Service Advantages

With extensive experience in proteomics and mass spectrometry, MtoZ Biolabs' Single Reaction Monitoring Analysis Service offers significant advantages in terms of accuracy, sensitivity, and quantitative stability. Our technical team is highly skilled and capable of tailoring analysis plans to meet the specific needs of clients, ensuring the efficiency and reliability of our services.

 

1.High Sensitivity

Our Single Reaction Monitoring Analysis Service can detect extremely low levels of target proteins in samples, making it particularly suitable for quantifying low-abundance proteins. Through precise peptide selection and optimized mass spectrometry parameters, MtoZ Biolabs helps clients identify and quantify proteins that are difficult to detect in complex biological samples.

 

2. High Selectivity

Since Single Reaction Monitoring only monitors characteristic ions of specific peptides, it effectively removes interference from other components in the sample. This results in high selectivity, ensuring more accurate quantification of target proteins, even when analyzing multiple samples.

 

3. High Reproducibility and Reliability

Our Single Reaction Monitoring Analysis Service provides excellent experimental reproducibility, ensuring consistent results for clients with every analysis. Whether performing multiple analyses on the same batch of samples or comparing different sample batches, our analyses maintain data stability and reliability.

 

4. Fast and Efficient

Compared to traditional full-spectrum mass spectrometry methods, Single Reaction Monitoring analysis is faster and more efficient. By focusing solely on the ions related to the target peptides, we reduce analysis time without compromising on data quality, enabling rapid and accurate results.

 

5.Advanced Analysis Platform

MtoZ Biolabs established an advanced Single Reaction Monitoring analysis  platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

6.One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

7.High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Single Reaction Monitoring analysis data, providing clients with a comprehensive data report.

 

 

Applications

1. Differential Protein Validation: Suitable for precise validation of iTRAQ differential expression proteins, ensuring the accuracy of results.

 

2. Subsequent Validation: Provides further validation and quantification of results from label-free quantitative proteomics.

 

3. Protein Quantification: Enables both absolute and relative quantification of proteins to meet diverse research needs.

 

4. Disease Biomarker Analysis: Supports quantitative analysis of disease biomarkers, construction of diagnostic models, and clinical validation.

 

5. Post-translational Modification Quantification: Accurately analyzes the quantitative levels of protein post-translational modifications such as phosphorylation and methylation.

 

6. Pathway Quantification Analysis: Performs quantitative analysis of key proteins in signaling pathways, uncovering regulatory mechanisms in biological processes.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

4. Data Analysis, Preprocessing, and Estimation (project-dependent)

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs' Single Reaction Monitoring Analysis Service is a high-precision protein quantification service based on mass spectrometry technology, designed to meet the diverse needs of research, drug development, and clinical studies. With our expertise and customized services, clients can obtain more accurate and reliable data to support their work. Please feel free to contact us for more details about our services!