Single Molecule Protein Sequencing Service
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Comprehensive Experimental Details
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Materials, Instruments, and Methods
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Total Ion Chromatogram & Quality Control Assessment (project-dependent)
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Data Analysis, Preprocessing, and Estimation (project-dependent)
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Bioinformatics Analysis
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Raw Data Files
Single Molecule Protein Sequencing is a cutting-edge analytical technology that directly sequences individual protein molecules at the single-molecule level, providing unparalleled insight into the primary structure of proteins. Unlike traditional protein sequencing methods that rely on large quantities of sample material and often involve complex sample preparation processes, this innovative technique allows for direct and precise identification of amino acid sequences from individual molecules, without the need for enzymatic digestion or amplification steps.
Restrepo-Pérez L. et al. Nat Nanotechnol. 2018.
Schematic of the Single-molecule Protein Sequencing Workflow
Proteins are among the most important biological macromolecules in living organisms, and their functions are closely tied to overall health. A protein’s functionality does not depend solely on its amino acid sequence but is also intimately linked to its three-dimensional structure, modifications, and interactions. Traditional proteomic methods, such as mass spectrometry (MS) and tandem mass spectrometry (MS/MS), often require large sample volumes and complex pretreatment steps, and these techniques cannot directly reveal the precise arrangement of each amino acid within the protein molecule. As technology continues to evolve, single-molecule protein sequencing services have emerged, offering a completely new perspective for protein research.
Service at MtoZ Biolabs
As a leader in the biotechnology field, MtoZ Biolabs is dedicated to providing cutting-edge technical solutions to researchers and biopharmaceutical companies worldwide. Our Single Molecule Protein Sequencing Service is built on an innovative single-molecule sequencing platform that breaks through the limitations of traditional protein sequencing methods by achieving high-precision amino acid sequence analysis at the single-protein-molecule level. This approach not only provides accurate data support for protein structure and function research but also reveals protein variations, modifications, and dynamic behavior within cells.
Through the Single Molecule Protein Sequencing Service, MtoZ Biolabs helps clients deeply explore protein diversity and its potential applications in disease, drug development, and vaccine design, driving precise and personalized development in life sciences and biomedicine. Drawing on years of technical expertise and industry experience, MtoZ Biolabs stands at the global forefront of single-molecule protein sequencing, delivering unprecedented efficiency for both scientific and clinical applications.
Comparison with Traditional Protein Sequencing Technology
In traditional protein sequencing, proteins are typically digested by enzymes into smaller peptide fragments, and the mass of these fragments is then analyzed by mass spectrometry to infer the protein sequence. This approach depends on large-scale protein samples and can lead to the loss of important information during the cleavage process. In contrast, single-molecule protein sequencing does not rely on peptide digestion or large sample volumes; it directly analyzes intact protein molecules, reducing information loss and sample preparation steps while greatly enhancing data integrity and accuracy.
Applications
1. Proteomics Research
The Single Molecule Protein Sequencing Service can be employed for qualitative analysis of complex proteins in proteomic studies, helping researchers uncover previously unknown protein sequences and modifications.
2. Structural Biology
For researchers exploring protein folding and structure-function relationships, single-molecule protein sequencing offers reliable amino acid sequence information, laying the groundwork for three-dimensional structure determination.
3. Cancer Research
This technology can detect mutations in cancer-related proteins and reveal their roles within cells, facilitating early diagnosis, targeted therapy, and drug development for cancer.
4. Drug Development
During new drug development, researchers can use the Single Molecule Protein Sequencing Service to identify the structural and functional characteristics of target proteins, accelerating both drug discovery and optimization.
5. Precision Medicine
By examining mutations and modifications of proteins in patients, single-molecule protein sequencing provides robust support for personalized treatment plans, particularly in precise diagnosis and targeted therapy.
What Could be Included in the Report?
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Protein Sequence Analysis Service
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