Single Molecule Sequencing Service
- Sample Types: Includes blood, tissue, cells, microorganisms, and other biological samples.
- Sample Volume: Samples should meet project-specific quality and quantity requirements. Refer to the sample submission guidelines for detailed instructions.
- Sample Storage and Transport: Samples should be stored at low temperatures and protected from freeze-thaw cycles to preserve DNA or RNA integrity.
Single molecule sequencing is a third-generation sequencing technology that directly sequences individual DNA or RNA molecules without the need for amplification. Unlike first-generation sequencing (Sanger sequencing) and second-generation sequencing (high-throughput sequencing), single molecule sequencing avoids the biases introduced by PCR amplification, offering improved accuracy and longer read lengths. This makes it particularly advantageous for complex genome assembly, mutation detection, and the analysis of repetitive regions.
First-generation sequencing (Sanger sequencing) provides high-accuracy results but suffers from low throughput and slower processing times. Second-generation sequencing, which utilizes high-throughput parallel sequencing, offers significantly improved speed and throughput, though it is limited by shorter read lengths and challenges in analyzing complex genomic regions. In contrast, single molecule sequencing’s longer read lengths make it a superior choice for tackling complex genomic analyses and mutation detection.
MtoZ Biolabs provides high-quality Single Molecule Sequencing Service that leverage PacBio single-molecule real-time (SMRT) and Oxford Nanopore platforms, enabling clients to obtain precise data and advance research in genomics, transcriptomics, and personalized medicine.
Technical Principles
Single molecule sequencing directly reads DNA or RNA molecules, bypassing amplification-induced biases. PacBio SMRT technology monitors the real-time insertion of bases during DNA polymerase synthesis to generate highly accurate genomic data. Oxford Nanopore technology detects sequence information by monitoring current changes as DNA or RNA molecules pass through nanopores. Single molecule sequencing provides continuous read lengths of thousands to tens of thousands of bases, making it highly suitable for complex genomic analyses, such as genome assembly and structural variant detection.
Escalante, A. E. et al. Rev Mex Biodivers. 2014.
Figure 1. The Principle of SMRT
Services at MtoZ Biolabs
MtoZ Biolabs offers state-of-the-art Single Molecule Sequencing Service that provide highly accurate, long-read sequencing data, making them ideal for comprehensive genome and transcriptome analysis. These advanced services enable:
· Genome Sequencing: High-accuracy genome assembly and mutation detection, ideal for complex genomic analyses.
· Transcriptome Analysis: Direct RNA sequencing to investigate gene expression, splicing variations, and regulatory mechanisms, supporting comprehensive transcriptomic studies.
· Complex Genome Analysis: Accurate sequencing of complex genomic regions, including repetitive sequences and structural variations.
We offer a complete range of services from data acquisition to interpretation of results. All sequencing data undergo rigorous quality control to ensure reliability and precision. Our team delivers detailed result reports tailored to client needs and offers further analytical support for deeper data interpretation.
Sample Submission Suggestions
To ensure the efficiency and accuracy of our Single Molecule Sequencing Service, clients need provide samples that meet the following standards:
MtoZ Biolabs provides detailed submission guidelines to ensure optimal sample quality for accurate results.
Why Choose MtoZ Biolabs?
MtoZ Biolabs combines extensive expertise in single molecule sequencing with cutting-edge technology platforms to deliver efficient, accurate sequencing services. Our key advantages include:
· Leading Technology Platforms: Utilization of PacBio SMRT and Oxford Nanopore technologies for advanced single-molecule sequencing capabilities.
· Customized Services: Tailored sequencing and data analysis solutions to meet client-specific research objectives.
· Expert Support Team: Experienced professionals providing continuous support and data interpretation throughout the project.
· Timely Service Delivery: We guarantee high-quality data delivery within agreed timelines and offer ongoing support for data analysis and interpretation.
Case Study
Case 1: Early Cancer Diagnosis Using Single Molecule Sequencing
This study employed PacBio SMRT sequencing to analyze cfDNA from HCC patients and found a higher proportion of long cfDNA molecules compared to healthy controls. The development of an HCC methylation score improved diagnostic accuracy, with AUC increasing from 0.75 to 0.91 when using long cfDNA molecules. This study demonstrates the value of Single Molecule Sequencing Service in cancer detection, providing an efficient early diagnostic tool.
Lois Choy, L. Y. et al. lin Chem. 2022.
Case 2: Single Molecule Sequencing in Plant Genomics
This research used single molecule sequencing to construct an enhanced melon genome reference, uncovering the dramatic expansion of transposable elements (TEs) in the melon genome.In-depth analysis showed that the expansion of these transposable elements could have important impacts on gene expression and function, especially in genome evolution and adaptation. This study highlights the strong application of Single Molecule Sequencing Service in plant genomics, providing important scientific evidence for the role of transposable elements in genomic regulation.
Castanera, R. et al. Front. Plant Sci. 2020.
MtoZ Biolabs’ Single Molecule Sequencing Service provide cutting-edge support for your genomics, transcriptomics, and personalized medicine research. By combining PacBio SMRT and Oxford Nanopore technologies, we deliver high-quality sequencing data and reliable analytical results. For any inquiries or further information, please contact MtoZ Biolabs for comprehensive technical support and service.
How to order?
