Single-Cell & Tissue Exosome Combined Proteomic Research Service

Single-cell & tissue exosome combined proteomic research is an advanced research strategy that integrates single-cell proteomics with proteomic analysis of tissue-derived exosomes, aiming to deeply investigate the relationship between cellular states and exosomal functions from both the microenvironment and secretory vesicle perspectives. This service utilizes single-cell quantitative proteomic technologies and high-resolution mass spectrometry platforms to systematically analyze the proteomes of various cell types within tissues as well as their secreted exosomes. By integrating these datasets, it reveals intercellular communication, signal transduction, and disease-related protein regulatory networks.

 

The single-cell & tissue exosome combined proteomic research service is widely applied in tumor microenvironment studies, immune regulation mechanism exploration, stem cell therapy research, neurological disease investigation, and regenerative medicine. It is particularly well-suited for elucidating the coupling between cellular functional states and their exosomal roles in highly heterogeneous tissues. Through integrated multi-dimensional proteomic analysis, this service facilitates the identification of key molecules and potential biomarkers, thereby advancing disease mechanism studies and the development of precision medicine.

 

Services at MtoZ Biolabs

Based on advanced platforms such as high-resolution mass spectrometry and flow cytometry, the single-cell & tissue exosome combined proteomic research service was launched by MtoZ Biolabs enables in-depth proteomic analysis of both single cells and exosomes. The service includes single-cell isolation from tissue samples, proteomic profiling, and extraction and proteomic analysis of tissue-derived exosomes. Using high-throughput and high-sensitivity quantification techniques, the service provides protein expression profiles at the cell subpopulation level and comprehensive exosomal protein composition, achieving multidimensional analysis of cellular behavior and secretory activity within the tissue microenvironment while ensuring data accuracy and reproducibility.

 

Service Advantages

1. Multidimensional Integrated Analysis

Simultaneously obtains protein expression profiles of tissue single cells and proteomic data of tissue-derived exosomes, enabling coordinated analysis of intracellular and extracellular protein signaling to comprehensively elucidate the relationship between cellular functions and secretory activities.

 

2. High-Resolution and High-Sensitivity Detection

Leveraging advanced platforms such as high-resolution mass spectrometry, this service enables precise quantification of low-abundance proteins, making it well-suited for analyzing rare cell populations in complex tissues and trace amounts of exosomes.

 

3. Standardized Workflow

A standardized operating procedure has been established covering sample processing, separation, detection, and data analysis, ensuring consistency across batches and reliability of research outcomes.

 

4. Flexible Customization

Analysis depth, protein marker panels, and data interpretation strategies can be tailored based on project objectives, supporting fundamental mechanistic studies, biomarker discovery, and the development of novel therapeutic strategies.

 

Applications

1. Tumor Microenvironment Mechanism Research

The single-cell & tissue exosome combined proteomic research service can be applied to reveal the synergistic roles of different tumor cell subpopulations and their secreted exosomes in tumor progression, metastasis, and immune evasion, aiding in the identification of key regulatory pathways and potential therapeutic targets.

 

2. Immunoregulation and Inflammatory Mechanism Exploration

By integrating the functional status of immune cells with their exosomal proteomes, this service enables in-depth analysis of their roles in inflammatory responses, autoimmune diseases, and immunotherapies.

 

3. Stem Cell Function Validation and Tissue Regeneration Research

The single-cell & tissue exosome combined proteomic research service can be used to evaluate the characteristics of stem cell subpopulations and the dynamic protein expression of their exosomes during tissue repair and regeneration, supporting the design of regenerative medicine strategies.

 

4. Biomarker Discovery and Validation

In various disease models, this service enables the identification of cell subpopulation-specific and exosome-associated protein features, facilitating the discovery of highly specific and sensitive diagnostic and prognostic biomarkers.

 

FAQ

Q1: What Types of Samples Are Suitable for this Service?

A1: We support analysis of both fresh and frozen tissue samples. To ensure efficient separation of exosomes and cellular components as well as protein stability, samples should be kept as intact as possible and avoid repeated freeze-thaw cycles.

 

Q2: How Is Data Quality Ensured?

A2: All experimental procedures are performed using high-end instruments and standardized protocols. QC samples and replicate experiments are included to ensure the accuracy, reproducibility, and comparability of the data.