Semi-Targeted Metabolites Profiling Service
- Disease biomarker discovery
- Drug development and pharmacometabolomics
- Clinical and translational research
- Food and nutrition science
- Agriculture and crop improvement
- Environmental and microbial studies
- Industrial biotechnology
- Detailed experimental information, including methods and conditions
- Raw data files
- Spectral and chromatographic outputs
- Data preprocessing and analysis results
- Bioinformatics analysis
- A tailored report based on project needs
MtoZ Biolabs provides a Semi-Targeted Metabolites Profiling Service designed to bridge the gap between untargeted metabolomics and fully targeted quantification. The Semi-Targeted Metabolites Profiling Service combines broad metabolite coverage with focused detection of biologically relevant compounds, enabling researchers to gain both discovery-driven and hypothesis-driven insights in a single workflow.
Metabolites are central intermediates and end products of biochemical processes that reflect the physiological and pathological state of organisms. Comprehensive study of metabolites, or metabolomics, has become an indispensable tool in biomedicine, agriculture, nutrition, and biotechnology. The semi-targeted metabolomics approach is a versatile strategy that integrates the strengths of both untargeted and targeted analyses within a single experiment. Similar to targeted workflows, it begins with the detection and measurement of a predefined set of metabolites relevant to the study. Beyond this, the acquired data can be revisited and mined retrospectively to capture broader metabolic variations that extend beyond the initial scope. This dual capability not only ensures reliable quantification of known metabolites but also uncovers unexpected yet biologically significant changes, offering deeper insights into cellular pathways and regulatory networks.
Mass spectrometry has emerged as the method of choice for semi-targeted metabolomics due to its sensitivity, accuracy, and ability to provide structural information. When coupled with chromatographic separation, it enables high-throughput profiling of metabolites across diverse biological samples. At MtoZ Biolabs, we employ advanced LC-MS/MS and HRMS platforms, supported by curated metabolite libraries and bioinformatics pipelines, to deliver reliable semi-targeted metabolite profiles.
Tronel, A. et al. Metabolomics. 2025.
Figure 1. Workflow for the Combined Approach of Semi-Targeted and Untargeted Metabolomics to Characterize Small Intestinal and Fecal Metabolomes
Service Advantages
✔️Balanced Approach
Combines the wide coverage of untargeted metabolomics with the accuracy of targeted analysis.
✔️Broad Metabolite Coverage
Detects and annotates thousands of metabolites across diverse chemical classes.
✔️High Sensitivity and Accuracy
Advanced LC-MS/MS and HRMS platforms ensure reliable identification and reproducible results.
✔️Biological Interpretation
Results are integrated with bioinformatics tools for pathway mapping and functional insights.
✔️Customizable Design
Flexible panels tailored to specific project objectives and sample types.
✔️Quality and Reproducibility
Strict quality control and technical replicates ensure consistent, publication-ready data.
Applications
Sample Submission Suggestions
1. Sample Types
Microbial cultures, cell cultures, animal tissues, plant tissues, animal biofluids, and other biological samples.
2. Storage and Transport
Samples should be snap-frozen and stored at –80°C, shipped on dry ice, and protected from repeated freeze–thaw cycles.
3. Notes
Avoid detergents, salts, preservatives, or other additives that may interfere with mass spectrometry detection.
Clients are encouraged to contact MtoZ Biolabs before sample preparation to receive tailored instructions for their specific project.
Deliverables
For more information about our Semi-Targeted Metabolites Profiling Service, please contact us. Our technical specialists are available to provide a free business assessment.
How to order?
