Semi-Quantitative Metabolites Analysis Service
- Detailed experimental information, including methods and conditions
- Raw data files
- Spectral and chromatographic outputs
- Data preprocessing and analysis results
- Bioinformatics analysis
- A tailored report based on project needs
MtoZ Biolabs offers a Semi-Quantitative Metabolites Analysis Service to help researchers evaluate relative changes in metabolite levels across biological samples. This service is built on advanced mass spectrometry platforms, which provide high sensitivity and broad coverage for diverse classes of metabolites.
Metabolomics is the study of all small molecules within cells, tissues, or organisms, and mass spectrometry has become one of its core analytical tools. Semi-quantitative metabolite analysis is a method that estimates relative abundance levels rather than providing exact absolute concentrations. The semi-quantitative metabolites analysis is particularly valuable because it lies between simple qualitative detection that only answers "present or absent" and fully quantitative measurement that delivers precise absolute values. By capturing relative differences, semi-quantitative analysis offers an efficient way to identify metabolic trends, screen potential biomarkers, and explore pathway alterations. It is well suited for exploratory studies where large sample sets need to be compared, providing a reliable overview that can guide subsequent targeted and absolute quantification.
Although semi-quantitative metabolite analysis is highly valuable for detecting metabolic trends, it does have limitations. Precision is lower than absolute quantification; signal responses may not always scale linearly with true concentrations, and results are generally comparable only within the same batch or platform. At MtoZ Biolabs, we address these challenges by implementing strict quality control, including technical replicates and pooled QC samples, to monitor instrument stability and correct for drift. In addition, key findings can be further validated with targeted metabolomics, ensuring that clients receive results that are both reliable and actionable.
Service at MtoZ Biolabs
MtoZ Biolabs provides semi-quantitative analysis of thousands of metabolites across diverse biological systems. The semi-quantitative metabolite profiling includes, but is not limited to, the following categories:
Analysis Workflow
Tong, F. et al. Food Res Int. 2025.
Figure 1. Semi-Quantitative Lipidomics Reveals the Characteristics of Lipid Metabolism in Sheep Milk Fermentation
Service Advantages
✔️Broad Coverage
Semi-quantitative profiling enables the detection of thousands of metabolites across diverse classes in a single analysis.
✔️High Sensitivity
Mass spectrometry allows reliable detection of metabolites even at low abundance, ensuring subtle changes are captured.
✔️Cost-Effective Approach
Provides wide-ranging metabolic insights without requiring isotope-labeled standards for every metabolite.
✔️Efficient Screening
Suitable for large sample cohorts, enabling rapid comparison across groups, treatments, or conditions.
✔️Pathway-Level Insight
Results highlight shifts in metabolic pathways, guiding further targeted validation or absolute quantification.
✔️Quality Assurance
Incorporation of technical replicates and QC samples ensures reproducibility and robustness of the data.
Sample Submission Suggestions
1. Sample Types
Microbial cultures, cell cultures, animal tissues, plant tissues, animal biofluids, and other biological samples.
2. Storage and Transport
Samples should be snap-frozen and stored at –80°C, shipped on dry ice, and protected from repeated freeze–thaw cycles.
3. Notes
Avoid detergents, salts, preservatives, or other additives that may interfere with mass spectrometry detection.
Clients are encouraged to contact MtoZ Biolabs before sample preparation to receive tailored instructions for their specific project.
Deliverables
For more information about our Semi-Quantitative Metabolites Analysis Service, please contact us. Our technical specialists are available to provide a free business assessment.
How to order?
