Sea Cucumber Polysaccharide Analysis Service

Sea cucumber polysaccharides are natural bioactive polysaccharide compounds found in the body wall, viscera, and body fluids of sea cucumbers. They possess complex branched structures and unique sulfation modifications. Studies have shown that these polysaccharides exhibit significant biological activities such as immunomodulatory, antioxidant, antitumor, antiviral, and tissue repair-promoting effects. Therefore, they have attracted great attention in the fields of marine drugs, functional foods, and health products.

 

With the increasing utilization of marine biological resources, the structural elucidation and functional studies of sea cucumber polysaccharides have become research hotspots. However, due to their complex molecular weight distribution, glycan heterogeneity, and multiple substitution modifications, analyzing their structural composition and functional mechanisms remains challenging.

 

Equipped with advanced analysis platforms, MtoZ Biolabs has established a systematic sea cucumber polysaccharide analysis service that enables comprehensive support from structural characterization to functional verification, providing reliable technical data and scientific insights for both academic and industrial clients.

 

Services at MtoZ Biolabs

1. Structural Analysis

MtoZ Biolabs offers a comprehensive structural analysis service covering all levels of sea cucumber polysaccharide characterization, from basic composition to advanced structural details. The service includes monosaccharide composition analysis, glycosidic linkage determination, sulfation and acetylation modification identification, branch distribution, and molecular weight measurement. By integrating multiple analytical technologies such as HPLC, gas chromatography–mass spectrometry (GC-MS), high-resolution LC-MS/MS, Fourier-transform infrared spectroscopy (FT-IR), and NMR, we can fully reveal the main-chain framework, branching patterns, and substitution features of sea cucumber polysaccharides, accurately identifying sulfation sites and glycan conformations. This comprehensive approach is suitable for both purified components and complex mixtures, providing reliable structural information for investigating structure–function relationships and bioactivity mechanisms.

 

2. Bioactivity Analysis

In bioactivity analysis, MtoZ Biolabs has established an integrated research platform from in vitro assays to cellular-level validation, allowing comprehensive evaluation of antioxidant, free radical scavenging, anti-inflammatory, immunomodulatory, and cell-proliferative activities of sea cucumber polysaccharides. We employ classical chemical systems such as DPPH, ABTS, and FRAP to measure antioxidant capacity, use macrophage and T-cell activation assays to analyze immunostimulatory effects, and apply MTT or CCK-8 assays to monitor cell proliferation and cytotoxicity. Additionally, flow cytometry and ELISA are used to quantify cytokine levels. By combining multi-angle quantitative results with structural data, we provide a scientific interpretation of the physiological functions and application potential of sea cucumber polysaccharides, supporting drug discovery, functional food development, and biopharmaceutical research.

 

Analysis Workflow

1. Sample Pretreatment: drying, desalting, and deproteinization to ensure sample compatibility with analytical instruments.

2. Monosaccharide Composition Analysis: determination of sugar types and ratios using HPAEC-PAD or GC-MS.

3. Glycan Structure Elucidation: LC-MS/MS and NMR for glycosidic linkage types, branching patterns, and substitution sites.

4. Modification Identification: FT-IR and high-resolution MS for sulfation and acetylation detection.

5. Activity Evaluation: in vitro assays for antioxidant and immunological activity quantification.

6. Data Analysis and Report Generation: integration of structure and activity data into a standardized technical report.

 

Service Advantages

1. Multi-Platform Integrated Analysis

MtoZ Biolabs integrates HPLC, GC-MS, LC-MS/MS, and NMR technologies to provide comprehensive analysis of sea cucumber polysaccharides with different molecular weights, polarities, and complexities, ensuring accuracy and completeness of structural information.

 

2. High-Sensitivity Detection

Using high-resolution mass spectrometry platforms such as Orbitrap and Q-TOF, we can accurately characterize glycan structures and modification features even from trace samples, meeting the needs of both research-grade and rare specimens.

 

3. Extensive Polysaccharide Analysis Experience

MtoZ Biolabs has accumulated years of research and technical expertise in polysaccharide analysis, covering marine, plant, and microbial polysaccharides. Our team is familiar with different types of polysaccharide compositions, glycosidic linkages, and modification patterns, with full-process experience from extraction and purification to high-resolution detection and data interpretation. Through extensive project experience, we have developed systematic analytical methods and characteristic spectral databases that enable high-quality, reproducible results, providing reliable scientific support for polysaccharide research and product development.

 

Applications

☑️ Marine Drug Research: exploring the antitumor, anti-inflammatory, and immunomodulatory mechanisms of sea cucumber polysaccharides

☑️ Functional Food Development: evaluating nutritional activity and health-promoting properties

☑️ Biomarker Discovery: analyzing chemical differences among species or production regions of sea cucumbers

☑️ Natural Product Structural Research: revealing the relationship between glycan modifications and biological functions

☑️ Quality Control and Standardization: establishing structural fingerprints and quality indicators for industrial applications

 

Deliverables

1. Comprehensive analysis report

2. High-resolution mass spectrometry and chromatogram data

3. NMR spectra and interpretation

4. Bioactivity test results and statistical analysis

 

MtoZ Biolabs provides a comprehensive and reliable sea cucumber polysaccharide analysis service to help you explore the molecular mechanisms and application potential of marine bioactive compounds. Contact us today to obtain a customized analysis plan and quotation, and advance your research with scientific precision and confidence.