SDS-Page Protein Purification Service

Proteins are fundamental to understanding biological systems, serving as enzymes, structural components, and signaling molecules. However, analyzing a specific protein often requires its isolation from a complex mixture of biomolecules. SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis) is a widely utilized technique for separating proteins based on molecular weight, providing an efficient and precise method for protein purification. At MtoZ Biolabs, we offer state-of-the-art SDS-PAGE Protein Purification Service, delivering high-quality results tailored to your research needs.

 



Portolano, N. et al. J Vis Exp. 2014.

SDS-PAGE showing the first step of the purification

 

 

Introduction to SDS-PAGE Protein Purification

SDS-PAGE is a robust electrophoretic method for resolving proteins in a sample. SDS, an anionic detergent, denatures proteins by disrupting non-covalent bonds and imparting a uniform negative charge. The proteins are then separated in a polyacrylamide gel matrix under the influence of an electric field, with smaller proteins migrating faster than larger ones.

 

When paired with protein extraction from the gel, SDS-PAGE serves as an effective purification strategy. This method is especially useful for isolating proteins for downstream applications such as mass spectrometry, enzymatic activity assays, or structural studies. Based on advanced operating procedures and a highly skilled team of professionals, the SDS-PAGE protein purification service provided by MtoZ Biolabs ensures precise separation and recovery of target proteins.

 

Why Choose SDS-PAGE for Protein Purification?

1.High Resolution and Precision: SDS-PAGE allows proteins to be separated with molecular weight resolution as fine as a few kilodaltons. This precision ensures the effective differentiation of proteins with similar sizes.

 

2. Adaptability to Complex Samples: SDS-PAGE can handle crude protein mixtures from various biological sources, including cell lysates, tissue homogenates, and extracellular fluids.

 

3. Broad Compatibility with Downstream Applications: The purified proteins can be used for an array of analyses, including Western blotting, protein sequencing, and functional assays.

 

4. Scalability: From analytical mini-gels to preparative formats, SDS-PAGE can be scaled to match the protein quantity required for your research.

 



Workflow of SDS-Page Protein Purification Service

 

Advantages of MtoZ Biolabs’ SDS-PAGE Protein Purification Service

✅ Cutting-Edge Equipment

Our laboratories are equipped with state-of-the-art electrophoresis and protein recovery systems, ensuring accuracy and consistency in results.

 

✅ Expertise in Protein Chemistry

Our team of highly skilled scientists brings years of experience in protein purification and analysis, offering technical insights to optimize your project.

 

✅ Tailored Protocols

Our SDS-PAGE protein purification service can customize every step in the purification process, including gel composition, staining method and recovery strategy, to meet diverse research needs.

 

✅ High Recovery Rates

Advanced recovery techniques and quality control measures ensure maximum yield and minimal loss of target proteins.

 

✅ Comprehensive Downstream Support

Beyond purification, we offer complementary services, including protein identification, functional assays, and structural analysis, to support your research objectives.

 

Applications of SDS-PAGE Protein Purification

SDS-PAGE purification finds applications across various research fields:

✅ Proteomics Research——Enables isolation of proteins for mass spectrometry-based identification and post-translational modification analysis.

 

✅ Structural Biology——Provides pure protein samples for crystallography, NMR, or cryo-EM studies.

 

✅ Functional Studies——Facilitates enzyme kinetics, protein-protein interaction assays, and binding studies.

 

✅ Antibody Production——Ensures antigen purity for raising specific antibodies.

 

✅ Diagnostics and Therapeutics——Used to purify biomarkers and therapeutic proteins for further validation.

 

Sample Submission Suggestions

To ensure successful purification, we recommend the following:

1. Protein Source: Cell lysates, tissue extracts, or other biological fluids.

 

2. Sample Volume: Minimum volume of 50 µL at a protein concentration of ≥1 mg/mL. Adjustments can be made based on project requirements.

 

3. Buffer Compatibility: Samples should be provided in compatible buffers (contact us for detailed guidelines).

 

*Note: Please contact us if you have any special requirements or need assistance with sample preparation.

 

Whether you are isolating a single protein or processing complex samples for proteomics, MtoZ Biolabs is your trusted partner. Contact us today to learn how our SDS-PAGE Protein Purification Service can advance your research endeavors. Together, we can achieve breakthrough discoveries in protein science.