Resources

Proteomics Databases



Metabolomics Databases

• • iTRAQ/TMT: Isotope Labeling and Tandem Mass Spectrometry Quantification

  iTRAQ/TMT (isotope labeling and tandem mass spectrometry quantification analysis) rely on the principle of using isotope tags to label proteins, enabling the simultaneous comparison of multiple samples in mass spectrometry analysis. iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and TMT (Tandem Mass Tag) are widely used isotope labeling and quantification techniques in tandem mass spectrometry. These methods allow researchers to conduct both relative and absolute quantification of protei......

• • Amino Acid Profile Analysis

  Amino acid profile analysis is a technique used to assess the types, concentrations, and proportions of various amino acids in biological samples, such as blood, tissues, and cell extracts, as well as in food samples. As fundamental building blocks of proteins, amino acids directly influence protein structure and function. This analysis enables researchers to determine both the relative and absolute quantities of amino acids present in a sample, unveiling potential biological functions and application......

• • Co-IP Protein Interaction

  Co-immunoprecipitation (Co-IP) is a classical biochemical technique used to study Co-IP protein interaction by isolating and detecting protein complexes from cell extracts. Using specific antibodies to target proteins, Co-IP enables researchers to analyze protein interactions and their roles in biological processes. This technique is widely applied in areas such as cell signaling, gene expression regulation, and cell cycle control, as it helps uncover complex protein networks and signaling pathways.  ......

• • Applications and Prospects of C-Terminal Sequencing in Proteomics

  C-terminal sequencing, a proteomics analytical method, specifically targets the identification of protein C-terminal sequences. The protein C-terminus is critical for its function, stability, degradation, and interactions. Therefore, obtaining an in-depth understanding of protein C-terminal sequences holds significant value in biological research. Recently, with continuous advancements in mass spectrometry (MS), the technology has made substantial progress and is widely applied in various proteomics f......

• • The Evolution of C-Terminal Sequencing: From Traditional to Modern Techniques

  C-terminal sequencing, the methodology for determining the sequences at protein C-termini (carboxyl termini), is a critical technique in proteomics. Compared to N-terminal sequencing, the analysis of C-terminal sequences has encountered numerous challenges, making its development a reflection of both technological advancements and the evolution of methods in protein chemistry research. From traditional chemical degradation to modern high-resolution mass spectrometry, this technology has undergone sign......

• • Hybridoma Sequencing

  Hybridoma sequencing involves the genomic sequencing of cells generated through hybridoma technology, which is crucial for exploring the genomic composition of hybridoma cells. These cells are produced in laboratories by fusing antibody-producing B lymphocytes with immortalized myeloma cells, resulting in cell lines capable of both indefinite proliferation and antibody secretion. This technique underpins the production of monoclonal antibodies, essential for creating cells that retain the specificity ......

• • Amino Acid Analysis LC-MS Plasma

  Amino acid analysis LC-MS plasma utilizes the combined strengths of liquid chromatography and mass spectrometry for precise detection and quantification of plasma amino acids. This technique enables accurate characterization of amino acid composition, concentration, and potential modifications. As essential metabolic components, plasma amino acids play critical roles in protein synthesis, energy metabolism, and neurotransmission. Measuring plasma amino acid levels with high accuracy is crucial for ass......

• • Lipidomics Pathway Analysis

  Lipidomics pathway analysis is a method for studying the intricate processes of lipid synthesis, degradation, and transformation in living organisms. Lipids, a diverse group of organic compounds including fats, phospholipids, and sterols, are ubiquitous in biological systems. This analysis focuses on lipid molecules and their metabolic pathways, aiming to elucidate their roles and mechanisms in biological functions. By employing lipidomics pathway analysis, researchers can gain deeper insights into di......

• • Glycopeptide Mapping

  Glycopeptide mapping is an analytical technique used to separate, identify, and analyze glycopeptides derived from glycoproteins following enzymatic or chemical cleavage. This method provides detailed information about glycopeptide structures, glycosylation sites, and glycan composition. Glycopeptides are macromolecules formed when sugar chains covalently bond to protein amino acid residues. Glycosylation plays a critical role in various biological processes, including cellular signal transduction, im......

• • Mass Spec Phosphorylation

  Mass spec phosphorylation analysis refers to the study of protein phosphorylation using advanced mass spectrometry techniques. The process begins with protein extraction from biological samples, such as cells or tissues. Due to the low relative abundance of phosphorylated proteins and the instability of phosphate groups, specialized enrichment methods, such as immunoprecipitation and chemical affinity chromatography, are commonly employed to isolate phosphorylated proteins or peptides.   Once enriched......