Resources

Proteomics Databases



Metabolomics Databases

• • Bradford Protein Concentration

  Bradford method, also known as the Coomassie Brilliant Blue method, is a fast and simple method for determining protein concentration. The method is based on the binding of proteins with a dye (Coomassie Brilliant Blue G-250), and protein concentration is determined by colorimetry.

• • How Much Protein Is Needed for Mass Spectrometry Sequencing?

  The amount of protein needed for mass spectrometry determination of protein sequence depends on several factors, including the sensitivity of the mass spectrometer, the purity of the sample, the type of analysis (such as peptide mapping, protein identification or post-translational modification analysis) and the properties of the specific protein. With advances in technology, the sensitivity of mass spectrometry is increasing and the amount of sample required is decreasing.

• • Protein Sequence Homology Analysis

  Protein sequence homology analysis is an important technique in bioinformatics, used to identify the similarity and evolutionary relationship between two or more protein sequences. Homology analysis is crucial for predicting the function of unknown proteins, studying the evolutionary history of proteins, and discovering new biological markers.

• • What Analysis Can Be Performed on Protein Sequencing Results?

  After obtaining the protein sequencing results, various analyses can be performed. These analyses help to reveal the function, structure, interactions and roles of the protein in the organism. The commonly used types of analysis mainly include:   1. Sequence Alignment Align with known protein sequences to identify homologous proteins, which helps to infer protein function and evolutionary relationships.   2. Functional Domain and Active Site Prediction Identify functional domains and active sites in protein

• • Can Protein Mass Spec Analysis Determine Amino Acid Aequences?

  Protein mass spectrometry analysis: An effective tool for obtaining amino acid sequences of proteins. Proteins are one of the most important functional molecules in living organisms, performing functions such as catalyzing biochemical reactions, constructing cellular structures, and regulating biological activities. Therefore, understanding the relationship between protein structure and function is crucial for biological and medical research.

• • Determination of Collagen Molecular Weight

  Collagen molecular weight determination is a technique used to determine the molecular weight of collagen, a complex protein. Collagen is one of the most abundant proteins in animals and is mainly found in the skin, bones, tendons, and connective tissues. Accurate determination of collagen molecular weight is of great significance in biomedical and cosmetic industries due to its critical role in cellular structure and tissue regeneration.

• • Application of TMT Labeling in Quantitative Proteomics Detection

  TMT (Tandem Mass Tag) labeling-based quantitative proteomics is an advanced mass spectrometry technique used to analyze and quantify protein expression differences in different samples. The core of this technique involves labeling protein samples with TMT tags. During this process, protein samples from different conditions or treatments are individually labeled with different TMT tags.

• • mRNA Capping Rate Detection

  mRNA capping efficiency detection is an experimental process used to evaluate the frequency and efficiency of cap structure addition at the 5' end of messenger RNA (mRNA) molecules. This cap structure is a special chemical modification that is crucial for the stability and translation efficiency of mRNA.

• • Infrared Spectroscopy is Used for Protein Sample Analysis

  Infrared spectroscopy analysis is a very important analytical technique widely used in fields such as chemistry, biology, and materials science. It is based on the principle of molecular absorption of specific wavelengths of infrared light, resulting in vibrational energy level transitions, thus obtaining molecular structural information of the sample. Proteins, as important macromolecules in living organisms, are essential for understanding biological processes.

• • How to lnterpret an lnfrared Spectrum

  The infrared spectrum is a technique that identifies chemical substances by analyzing the changes in the vibration frequency of chemical bonds in molecules. Analyzing an IR spectrum primarily involves identifying characteristic absorption peaks, which correspond to the vibration frequencies of different functional groups in the molecule.