Resources

Proteomics Databases



Metabolomics Databases

• • Yeast Proteomics Analysis Strategy

  In the field of biotechnology and molecular biology, yeast (especially the brewing yeast Saccharomyces cerevisiae) has become an extremely important model organism due to its ease of cultivation, relatively simple genome, and remarkable similarity to higher organisms. It is not only widely used in industrial production and fermentation processes, but also provides an outstanding model for understanding the biological processes of more complex organisms.

• • AI and Multiplex Cloning Redefine Aantibody Discovery

  Antibodies, as important biopharmaceuticals, play a significant role in the treatment of cancer, autoimmune diseases, and infectious diseases. Antibody discovery, as a crucial process in antibody drug design, has witnessed the application of numerous new technologies. However, antibody research still faces multiple challenges such as affinity, specificity, stability, and immunogenicity.

• • Lipidomics Study of Escherichia Coli Metabolism

  In modern life science research, lipidomics has become an indispensable technique for understanding cell functions, disease mechanisms, and drug action mechanisms. Lipids, as important components in organisms, not only play a key role in the structure and function of cell membranes, but also participate in various biological processes such as energy storage, signal transduction, and cell death.

• • Metabolic Flux Analysis of Blood Cells

  A deep understanding of cellular metabolism is particularly important in modern biomedical research and clinical diagnostics. This is because cellular metabolic processes are directly related to the physiological state of cells, the onset and development of disease, and the response to treatment.

• • CD Spectroscopy of Protein Conformational Changes

  Introduction:In biology, the structure and function of proteins are closely related. Therefore, understanding and observing the conformational changes of proteins are crucial for unraveling their biological functions. This article mainly discusses how to observe and analyze protein conformational changes using circular dichroism spectroscopy.

• • Plant Stem Vein Protein Isoelectric Point Analysis Based on cIEF

  Plant stem vein-based protein isoelectric point analysis based on capillary isoelectric focusing (cIEF) is a refined biotechnology specifically used to investigate the isoelectric points (pI) of proteins within plants. This technique is particularly important in plant science research as it not only reveals the molecular characteristics of proteins but also helps researchers gain a deeper understanding of plant physiological processes.

• • Protein Mass Spectrometry Analysis Experimental Tips for Success

  Mass spectrometry is an important technique in modern proteomics research, with a crucial role in revealing the structure, function, and interaction of proteins. However, the success of mass spectrometry experiments often relies on the skill and experience of the experimenter. This article will share some tips and suggestions for successfully conducting mass spectrometry experiments, helping readers improve experimental efficiency and reduce errors.

• • Protein Purity Analysis in E. coli Using Chromatography Techniqu

  Protein purity analysis of Escherichia coli is a crucial step in biotechnology and molecular biology research, especially in the fields of protein engineering, drug development, and proteomics. This technique primarily utilizes two chromatographic methods, molecular sieving (gel filtration) and reverse-phase chromatography (RP-HPLC), to separate and purify proteins from Escherichia coli, ensuring the obtainment of high-purity protein samples for subsequent structural and functional analysis.

• • Following Up on Proteins Identified by Mass Spectrometry

  For differentially expressed proteins identified by mass spectrometry, the following steps are generally involved in subsequent studies: 1. Identification and validation of target proteins: Differentially expressed proteins identified by mass spectrometry form a relatively broad protein collection, which may include various differentially expressed proteins. Therefore, the first step is to determine the target proteins for study.

• • Quantitative Proteomic Study of Yeast Phosphorylation

  Yeast, as a model organism in biological research, has always played an important role in fields such as genetics, biochemistry, and cell biology. Its simple genome structure and abundant genetic resources make it an excellent research subject for functional gene identification, metabolic regulation, and signal transduction. In this context, quantitative phosphoproteomics studies provide a powerful tool for exploring the function and regulatory mechanisms of phosphorylated proteins in yeast.