Resources

Proteomics Databases



Metabolomics Databases

• • Advantages and Disadvantages of Glycosylation Site Analysis Using Enzyme Digestion and 18O Labeling

  Glycosylation is a crucial post-translational modification of proteins, affecting protein stability, function, and cell interactions. Accurate analysis of glycosylation sites is essential for understanding their role in biological processes. Among the various glycosylation analysis methods, enzymatic digestion and 18O labeling have been widely used for glycosylation site analysis due to their high accuracy and sensitivity.

• • Principle of Glycosylation Site Analysis Based on LC-MS/MS

  Glycosylation is a common and significant post-translational modification (PTM) of proteins, playing a critical role in cellular communication, immune response, protein folding, and disease progression. The study of glycosylation is crucial for understanding the pathological mechanisms of various disorders, such as cancer, metabolic dysfunction, and neurodegenerative diseases.

• • Mechanism of Glycoprotein Structural Analysis via Mass Spectrometry

  Glycoproteins are proteins covalently attached to one or more carbohydrate chains. They are widely present on the cell surface and in secreted proteins, playing vital roles in cell recognition, signal transduction, and immune response. Due to the complexity and diversity of glycan structures, elucidating glycoprotein structures is crucial for understanding their biological functions.

• • Application of Mass Spectrometry in Glycoprotein Analysis

  Glycoproteins are essential biomolecules involved in various biological processes, including cell communication, immune responses, protein folding, and molecular recognition. Glycosylation, a key post-translational modification, significantly influences the function and stability of glycoproteins. Hence, precise and sensitive analysis of glycosylation is crucial to understanding the biological roles of glycoproteins.

• • Workflow of Glycoprotein Detection Using LC-MS

  Glycoproteins play a critical role in various biological processes, including cell recognition, signal transduction, and immune responses, primarily due to their glycosylation modifications. Studying these glycosylation patterns can help elucidate molecular mechanisms behind diseases such as cancer and immune disorders. LC-MS (Liquid Chromatography-Mass Spectrometry) is a widely used technique for detecting and characterizing glycoproteins' glycosylation modifications.

• • Advantages and Disadvantages of Glycoprotein Analysis Methods

  Glycoproteins are a class of proteins that are widely present in living organisms and play essential roles in biological processes such as protein regulation, cell communication, and immune responses through glycosylation. Changes in glycosylation are often associated with various diseases, including cancer and metabolic disorders.

• • Principle of Glycoprotein Analysis Based on Mass Spectrometry

  Glycoproteins are biomolecules where proteins are covalently bonded with carbohydrate chains, playing vital roles in cellular recognition, signal transduction, immune regulation, and other biological processes. Glycosylation, one of the most complex post-translational modifications, significantly affects the structure, stability, and function of proteins. Hence, accurate analysis of glycoproteins is critical for understanding biological processes and developing disease biomarkers.

• • Mechanism of N-glycan Analysis Using HILIC-UHPLC

  N-glycosylation is a crucial post-translational modification of proteins, widely present in eukaryotic cells. It plays an important role in various biological processes, such as protein folding, stability, cell recognition, and signal transduction. Therefore, accurate analysis of protein N-glycosylation is essential for understanding many biological phenomena and disease mechanisms. N-glycan analysis involves the separation, identification, and quantification of glycan structures.

• • Protein Amino Acid Composition Analysis: Methodology and Practice

  Protein is one of the most important molecules in living organisms and plays a critical role in the structure and function of cells. Understanding the amino acid composition of proteins is crucial for understanding their function and structure.

• • Practical Guide to Monosaccharide Composition Analysis: Enhancing Sample Analysis Efficiency

  Monosaccharides are the basic units that make up polysaccharides and are of great importance in the field of biopharmaceutical research and development. Monosaccharide composition analysis is a key technique that can help scientists understand the structure and characteristics of polysaccharides. However, traditional methods for monosaccharide composition analysis suffer from low efficiency.