Resources

Proteomics Databases



Metabolomics Databases

• • Protein Mass Analysis

  Protein mass analysis is a crucial analytical technique used to determine the molecular mass and structural properties of proteins. The core principle of this method is to precisely measure the molecular mass of proteins, thereby revealing their structural characteristics, functional roles, and involvement in biological processes. Protein mass analysis is widely applied in various fields of life sciences, including proteomics, biomarker discovery, and drug development.   Protein mass analysis is based......

• • Molecular Weight Determination by GPC

  Molecular weight determination by GPC is a chromatographic technique used for analyzing and characterizing the molecular weight distribution of polymers. It is based on the principle of size exclusion, where solute molecules are separated by the screening effect of the stationary phase's porous material. High molecular weight solutes, which cannot penetrate the pores, elute earlier, while low molecular weight solutes, which are able to enter and remain trapped in the pores for longer, elute later. Thi......

• • Native Protein Analysis

  Native protein analysis is a technique used to study the functions and characteristics of proteins in their natural state. This method preserves the native conformation and interactions of proteins, enabling scientists to directly observe and measure their biological activity. Unlike analyses conducted under denaturing conditions, which often disrupt the protein’s natural structure and may lead to the loss of functional properties, native protein analysis offers distinct advantages in studying protein......

• • Gel Filtration Molecular Weight Determination

  Gel filtration molecular weight determination is an important chromatographic technique used to analyze molecular weight and its distribution, widely applied in the study of biomacromolecules such as proteins, polysaccharides, and polymers. The basic principle of gel filtration molecular weight determination relies on size exclusion in porous materials, achieving separation based on differences in molecular size. Larger molecules, which cannot enter the pores of the gel particles, elute from the colum......

• • GPC Analysis Molecular Weight

  Gel permeation chromatography (GPC) is a crucial technique for gpc analysis molecular weight and distribution in polymer materials. In gpc analysis molecular weight determination, the sample is dissolved in an appropriate solvent and passed through a chromatographic column filled with porous packing material. This physical separation process allows GPC to provide detailed information about the molecular weight and distribution of the sample, which is essential for studying the physical and chemical pr......

• • GPC for Molecular Weight Determination

  The GPC for molecular weight determination is a widely used technique in polymer science for analyzing the molecular weight and distribution characteristics of polymers. This method is based on the principle of gel permeation chromatography (GPC), which separates polymer molecules by size.   The basic principle of GPC for molecular weight determination is the size exclusion mechanism. When polymer molecules in the sample solution pass through a chromatographic column filled with porous materials, thei......

• • Determine Molecular Weight from Mass Spectrum

  Mass spectrometry is a powerful analytical technique that allows researchers to determine molecular weight from mass spectrum with high accuracy, making it especially valuable for the study of biological macromolecules such as proteins, peptides, and nucleic acids. The principle of this method involves ionizing the sample molecules and analyzing their movement in an electric or magnetic field to calculate their molecular weight. The ability to determine molecular weight from mass spectrum plays a cruc......

• • Gel Permeation Chromatography for Molecular Weight Determination

  Gel permeation chromatography (GPC) is a widely used separation technique for determining the molecular weight and distribution of macromolecules. This method relies on the permeation and exclusion effects of molecules within a porous stationary phase, where separation is achieved based on differences in molecular volume. Due to its efficiency, rapidity, and stability, gel permeation chromatography for molecular weight determination has found extensive applications in polymer science, biochemistry, an......

• • MALDI-TOF Peptide Mass Fingerprinting

  MALDI-TOF Peptide Mass Fingerprinting (MALDI-TOF PMF) offers a rapid and highly accurate method for identifying and quantifying protein compositions in complex samples by detecting peptide masses and their relative abundances.   MALDI-TOF peptide mass fingerprinting combines matrix-assisted laser desorption/ionization (MALDI) with time-of-flight mass spectrometry (TOF-MS). Laser-induced desorption and ionization of peptides generate charged peptide ions from the sample. These ions are then analyz......

• • Molecular Weight Determination by Gel Filtration Chromatography

  The principle of molecular weight determination by gel filtration chromatography relies on the differential diffusion rates of molecules within gel-filled matrices. Larger molecules, unable to penetrate the pores of gel particles, elute earlier from the chromatography column. Conversely, smaller molecules diffuse into the gel pores, resulting in longer retention times and delayed elution. By accurately measuring the sample's elution time (or volume), the molecular weight can be determined. This proces......