Resources

Proteomics Databases



Metabolomics Databases

• • Detection of O-Glycans by MALDI-TOF-MS

  O-glycans, a common type of glycosylation modification, are widely present on the surface of proteins and play critical roles in various biological processes such as cell recognition, signal transduction, and immune responses. Due to the structural complexity and high heterogeneity of O-glycans, mass spectrometry-based methods have become indispensable tools for analyzing glycoproteins and studying glycosylation patterns.

• • Detection of Glycosylation Sites and Glycoforms Based on LC-MS/MS

  Glycosylation is a crucial post-translational modification that influences protein folding, stability, and function. Accurate detection of glycosylation sites and glycoforms is essential for studying biological processes, disease mechanisms, and drug development. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the primary method for detecting glycosylation sites and glycoforms due to its high sensitivity and resolution.

• • Mechanism of Proximity Extension Assay in Olink Analysis

  Olink analysis is a highly sensitive protein detection platform with broad applications in biomarker discovery, clinical research, and drug development. One of its core technologies is the Proximity Extension Assay (PEA), which utilizes antibody recognition combined with DNA extension to detect hundreds of proteins in complex biological samples simultaneously.

• • Application of Olink Proteomics in Biomarker Discovery

  Olink proteomics technology, known for its highly sensitive and precise protein quantification capabilities, has become a pivotal tool in the field of biomarker discovery. By combining multiplex immunoassays with high-throughput liquid chip technology, Olink allows the quantification of a large number of proteins in a single experiment. This not only shortens research timelines but also improves the accuracy and reproducibility of results.

• • Advantages and Disadvantages of Olink Analysis

  Olink analysis is a cutting-edge proteomics tool based on Proximity Extension Assay (PEA) technology, increasingly applied in biomedical research. Its combination of high sensitivity, specificity, and high-throughput detection allows the measurement of hundreds of proteins from minimal sample volumes. Below, we explore in detail the advantages and disadvantages of this technology.

• • Workflow of Olink Proteomics

  Olink proteomics is a high-throughput technique leveraging protein-specific antibodies, widely used in life sciences for the simultaneous detection and quantification of numerous proteins. This method uses a unique immuno-amplification mechanism, enabling the analysis of hundreds to thousands of target proteins in a single assay, which is especially useful in clinical and basic research.

• • Principle of Olink Proteomics

  Olink proteomics is an advanced technology based on Proximity Extension Assay (PEA), allowing the simultaneous measurement of multiple target proteins with exceptional sensitivity and specificity. The core concept of PEA involves the use of antibodies that specifically bind to target proteins, coupled with precise protein quantification using DNA sequence tags attached to the antibodies.

• • Mechanism of Chemical Proteomics Analysis

  Chemical proteomics is a research approach that combines chemical tools with mass spectrometry to study protein function, interactions, and modification states. The central mechanism involves using chemical probes, reactive compounds, or labeling molecules to identify proteins or their modification sites, followed by qualitative and quantitative analysis using mass spectrometry.

• • Application of Chemical Proteomics Analysis

  Chemical proteomics, a technique that utilizes chemical probes and targeted reactions to detect and functionally analyze proteome components, has found broad applications in the biomedical field. With advancements in analytical techniques, chemical proteomics has become a powerful tool for biological research and drug development. Its application spans from target identification to biomarker discovery, advancing research in precision medicine and drug development.

• • Principle of Chemical Proteomics Analysis

  Chemical proteomics is an emerging field that leverages the interaction between chemical probes and proteins to study protein function, structure, and activity within the cellular environment. By integrating chemical synthesis with proteomics techniques, it allows the identification, quantification, and functional analysis of proteins through specific chemical modifications or labeling.