Protein Analysis via Mass Spectrometry
Protein analysis via mass spectrometry is a widely used technique in proteomics and includes the following steps:
Sample Preparation
Proteins are extracted and purified through methods such as gel electrophoresis and liquid chromatography.
Protein Digestion
Proteins are enzymatically digested into peptide fragments, typically using enzymes like trypsin.
Mass Spectrometry Analysis
The peptide fragments are ionized, with common methods being MALDI and ESI. Their mass-to-charge ratio (m/z) is then measured using mass spectrometers such as TOF, Q-TOF, or Ion Trap.
Data Analysis
The mass spectrometry data are processed using specialized software and compared against protein databases to identify the peptide sequences and assign them to corresponding proteins.
Applications of this technique include protein identification, quantitative analysis, and studies on post-translational modifications.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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