Resources
Proteomics Databases
Metabolomics Databases
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Affinity purification-mass spectrometry (AP-MS) integrates affinity purification techniques with mass spectrometry to investigate molecular interactions, the composition of protein complexes, and the dynamic behavior of functional biomolecules. This method leverages the specific binding properties of target molecules, such as proteins, nucleic acids, or small molecules, to isolate them from complex biological samples while simultaneously capturing associated interacting molecules. Subsequent identific......
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The ligand binding assay is a fundamental methodology used to investigate interactions between biomolecules and ligands, providing critical insights into binding properties and kinetic parameters. Ligands, which may include small molecules, proteins, nucleic acids, or other chemical compounds, often play essential roles in biological processes such as enzymatic catalysis, signal transduction, and molecular transport. By assessing parameters such as binding affinity (e.g., dissociation constant), bindi......
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Ligand assay is a pivotal technique for investigating interactions between biomolecules and their ligands, enabling the determination of critical parameters such as binding mechanisms, kinetic profiles, and affinity. Ligands, which include small molecules, proteins, nucleic acids, or other chemical entities, bind specifically to target molecules. This binding often governs essential biological processes such as enzymatic catalysis, signal transduction, metabolic regulation, and gene expression. By qua......
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• Immunoprecipitation Analysis
Immunoprecipitation analysis is a technique that utilizes the specific binding between antigens and antibodies to isolate and enrich target proteins or their complexes from complex biological samples. By capturing the target protein with a specific antibody and applying elution and detection methods, this technique facilitates the separation and functional analysis of proteins. It is a versatile and widely applied tool in molecular biology, proteomics, and biomedical research, particularly for explori......
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Proximity Labeling is an innovative biological technique designed to investigate spatial protein interactions and molecular relationships within the cellular microenvironment. This approach leverages enzymes capable of catalyzing proximity-specific reactions, such as biotin ligase or peroxidase, to covalently attach distinct markers (e.g., biotin or fluorescent molecules) to molecules in the vicinity of target proteins. These labeled molecules are subsequently identified and quantified using mass spec......
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• Methods for Protein Structure Elucidation and Their Roles in Research
Methods for protein structure elucidation and their roles in research are foundational to understanding the molecular mechanisms of biological processes. Techniques such as X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and cryo-electron microscopy (Cryo-EM) have enabled researchers to investigate protein architectures with unprecedented precision. These methodologies provide detailed insights into protein function and dynamics, driving progress in fields such as drug discovery,......
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• Protein Structure Analysis Methods and Applications
Protein structure analysis methods and applications play a pivotal role in understanding biological functions and designing biomedical solutions. This article explores key methods and their advantages, limitations, and broader applications in research and industry. X-ray Crystallography The three-dimensional atomic structure of a protein is determined by analyzing the diffraction patterns generated when X-rays interact with protein crystals. 1. Advantages Provides high-resolution structural data, ......
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• Protein Structure Determination Techniques and Applications
Protein structure determination techniques and applications are foundational to elucidating the molecular mechanisms of life. These methodologies, encompassing X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and cryo-electron microscopy (Cryo-EM), have profoundly impacted research in biomedicine and pharmaceutical innovation. By leveraging these advanced techniques, researchers can uncover intricate protein architectures essential for biological function and therapeutic intervent......
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Inductively Coupled Plasma Metal Analysis (ICP Metal Analysis) is a technique for detecting and quantifying trace elements and metal contaminants in samples. The principle involves atomizing and ionizing the sample with an inductively coupled plasma, followed by detection of the ions using mass spectrometry. ICP Metal Analysis is renowned for its high sensitivity and multi-element detection capability, allowing for the identification of dozens of elements at ultra-low concentrations. This makes it wi......
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• How to Determine the Amino Acid Sequence of Unknown Proteins
The process to determine the amino acid sequence of unknown proteins involves a structured series of steps, including protein purification, enzymatic digestion, LC-MS/MS analysis, and database searching. Protein Purification To enable accurate analysis, the target protein must first be purified from complex biological samples. Techniques such as centrifugation, precipitation, and chromatography are commonly employed to isolate the protein of interest. Enzymatic Digestion Proteins are enzymatically......
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