Resources

Proteomics Databases



Metabolomics Databases

• • SILAC

  Stable isotope labeling by cell culture (SILAC) is a technique used to study proteomics, metabolic pathways, and cellular biological processes. SILAC involves labeling cellular proteins with amino acids containing stable isotopes during cell culture, allowing for the distinction of labeled proteins from unlabeled ones in mass spectrometry analysis. This method provides a powerful tool for researchers to quantify proteins, examine protein-protein interactions, and investigate functional changes, with ......

• • Cyclic Peptide Synthesis

  Cyclic peptide synthesis refers to the chemical or biotechnological processes used to create peptides with a cyclic structure. These cyclic peptides are structurally diverse and exhibit notable biological activity, making them highly applicable in fields such as drug development, agriculture, and biochemical research. For instance, cyclosporine, an important immunosuppressant, is widely used in organ transplants to prevent rejection, while cucurbitacin has demonstrated potential as an anticancer agen......

• • Quantitative Protein Profiling

  Quantitative protein profiling integrates mass spectrometry technology with bioinformatics analysis to accurately measure the abundance and changes in protein levels within samples. This technique provides researchers with detailed insights into protein expression in biological samples, which is crucial for understanding cellular processes, disease mechanisms, and drug development. In basic research, quantitative protein profiling enables scientists to uncover dynamic alterations in protein networks ......

• • Solid Phase Polypeptide Synthesis

  Solid phase polypeptide synthesis (SPPS) is a chemical synthesis technique used to construct polypeptides with specific sequences. First introduced by Robert Bruce Merrifield in the 1960s, this method revolutionized traditional liquid-phase synthesis by fixing the initial amino acid onto an insoluble solid support (usually resin) and adding subsequent amino acids step by step. In each synthesis cycle, unreacted reagents and by-products are removed through simple washing, thus enhancing the ease and e......

• • Proximity Extension Assay PEA Technology

  Proximity extension assay PEA technology is a highly sensitive and specific molecular analysis method used to detect and quantify target proteins in proteomics. This technique utilizes a pair of specific antibodies that bind to the target protein, followed by DNA sequence ligation and amplification, enabling ultra-high sensitivity and specificity in protein detection. The core principle of proximity extension assay PEA technology relies on a phenomenon called the "proximity effect," whereby the DNA s......

• • Solid Phase Peptide Synthesis

  Solid phase peptide synthesis (SPPS) is a widely used chemical method for the stepwise synthesis of peptides on an insoluble solid support. First introduced by Robert Bruce Merrifield in 1963, solid phase peptide synthesis (SPPS) has become a fundamental technique in biochemistry and biotechnology. The process involves sequentially adding amino acids to a solid phase resin, greatly enhancing both the efficiency and purity of peptide synthesis. SPPS has broad applications, including drug development, ......

• • Quantitative Proteomics in Disease and Drug Discovery

  Quantitative proteomics is an advanced technique designed to accurately measure and compare protein expression levels across different samples. Unlike traditional proteomics methods, quantitative proteomics not only identifies proteins but also provides detailed information on their relative or absolute abundances under various biological conditions. One of the primary advantages of this technique is its ability to capture dynamic changes in protein levels, which often reflect the physiological or pat......

• • Protein Analysis Enhancing Scientific Discovery

  Protein analysis plays a pivotal role in elucidating the molecular mechanisms underlying biological processes. By thoroughly examining proteins, researchers gain insights into their structure, function, interactions, and variations across biological contexts. This knowledge is crucial for advancing fundamental research, improving disease diagnostics, and developing novel therapeutics. A major challenge in protein research lies in the efficient separation and extraction of samples. Given the diversity ......

• • Top Down Proteomics for Protein Analysis

  Top down proteomics is an advanced technology that has been gaining increasing prominence in the field of protein research. Compared to traditional proteomics methods, this approach analyzes intact proteins directly, avoiding the conventional enzymatic digestion step, and thus provides more detailed and comprehensive protein information. It offers distinct advantages, particularly in the study of protein post-translational modifications, protein variants, and structural complexities. This technology i......

• • MALDI-TOF Mass Spectrometry in Proteomics

  MALDI-TOF Mass Spectrometry integrates Matrix-Assisted Laser Desorption/Ionization (MALDI) with Time-of-Flight Mass Spectrometry (TOF-MS). Since its introduction by Karas and Hillenkamp in 1988, this technique has become a cornerstone of modern mass spectrometry due to its high resolution, broad mass range, and rapid analytical capabilities. It has been extensively utilized in proteomics, metabolomics, clinical diagnostics, environmental monitoring, and forensic investigations.   The MALDI-TOF Mass Sp......