Resources

Proteomics Databases



Metabolomics Databases

• • DSC and TGA Analysis

  DSC and TGA analysis are widely used thermal characterization techniques in fields such as materials science, biology, and chemistry. DSC is primarily employed to assess the thermal properties of materials by recording the heat flow difference between the sample and the reference during temperature changes. This technique is used to investigate thermal behaviors such as phase transitions, glass transitions, crystallization, and melting. TGA, on the other hand, measures the relationship between sample......

• • De Novo Sequencing in Proteomics Research

  De novo sequencing, a method widely used in proteomics research, allows for the determination of the amino acid sequence of unknown or partially known proteins. This technique is essential in scientific exploration and biomedical research because it overcomes the limitations of traditional database-dependent methods, enabling the direct derivation of protein sequences from raw mass spectrometry data. De novo sequencing is particularly useful for studying new species, phenotypic variations, or genomic......

• • N-terminal Sequencing in Proteomics

  N-terminal sequencing is a critical technique used to analyze the primary structure of proteins, specifically aimed at identifying the amino-terminal (N-terminal) sequence of a protein or peptide chain. As a key approach in proteomics research, this technology is widely employed for protein structure identification, functional studies, sample purity validation, and the investigation of post-translational modifications. By precisely analyzing the amino acid sequence, N-terminal sequencing provides val......

• • Edman Protein Sequencing

  Edman protein sequencing is a precise method for determining the amino acid sequence of proteins by sequentially identifying the N-terminal residues of a polypeptide chain, thereby establishing the protein's primary structure. Introduced by Pehr Edman in 1950, it has since become a cornerstone in biochemical and molecular biology research. The technique relies on the Edman degradation reaction, where the N-terminal amino acid of the protein or polypeptide reacts with phenylisothiocyanate (PITC) to for......

• • Size-Exclusion Chromatography Protein Purification

  Size-exclusion chromatography protein purification is a technique used to separate and purify proteins based on their differences in molecular size. It utilizes porous materials packed within a column, functioning as a sieve that allows components of a mixture to pass through at varying rates, depending on their size, leading to separation. The core principle of this technique is the volume exclusion effect, where larger molecules cannot enter the pores or can only enter larger pores, causing them to ......

• • Size Exclusion Chromatography for Protein Purification

  Size exclusion chromatography for protein purification is a widely utilized method in biochemical and proteomics research, allowing the separation of molecules based on differences in size and shape. This technique is essential for ensuring the accuracy and reliability of subsequent analyses, particularly in proteomics. Also known as gel filtration chromatography, the method uses columns packed with porous beads to separate proteins in a mixture. Larger molecules are excluded from the bead pores and e......

• • Antibody Profiling

  Antibody profiling refers to the detailed study and characterization of the structure, function, and properties of antibodies. As essential components of the immune system, antibodies specifically recognize and bind to antigens, thereby contributing to immune defense. In biomedicine, antibodies are used not only as diagnostic tools but also for the treatment of various diseases, including cancer, autoimmune disorders, and infections. Consequently, antibody profiling plays a pivotal role in drug develo......

• • Protein Sequence Analysis Overview

  Protein sequence analysis involves determining the amino acid sequence and chemical modifications of proteins using advanced methodologies. Proteins are essential macromolecules that adopt complex three-dimensional structures derived from their linear amino acid sequences, enabling them to perform diverse cellular functions. The primary objective of protein sequence analysis is to elucidate the primary structure of proteins, providing critical insights into their function, structure, and interactions.......

• • Protein Sequencing by Mass Spectrometry

  Protein sequencing by mass spectrometry is a powerful method for determining the amino acid sequence of proteins. Proteins play central roles in biological processes, with their structure and function directly influencing an organism's growth, development, and metabolism. Understanding the amino acid sequence of proteins provides crucial insights into their functional mechanisms. Traditional methods such as Edman degradation, while accurate, have been largely supplanted by mass spectrometry-based tech......

• • Protein Characterization Techniques

  Protein characterization techniques comprise a diverse array of methodologies that facilitate in-depth understanding of protein structure, function, and interactions. Key techniques include mass spectrometry, nuclear magnetic resonance (NMR) spectroscopy, X-ray crystallography, circular dichroism (CD), and cryo-electron microscopy (Cryo-EM). Mass spectrometry is indispensable in the identification and quantification of proteins, particularly within proteomics, enabling the rapid and precise analysis o......