Resources

Proteomics Databases



Metabolomics Databases

• • Affinity Purification-Mass Spectrometry

  Affinity Purification-Mass Spectrometry (AP-MS) is an integrated method that combines specific protein separation techniques with high-sensitivity molecular detection, widely applied in proteomics research. The principle of this technique involves selectively isolating target proteins and their associated molecules from complex biological samples through affinity purification, followed by identification and quantification using mass spectrometry. Affinity purification relies on high-specificity inte......

• • Competitive Ligand Binding Assay

  Competitive ligand binding assay is an experimental technique used to elucidate the binding mechanisms and biological functions of small molecules, drugs, or other ligands by analyzing their competition with biomacromolecules such as proteins, DNA, or RNA. This method is integral to drug discovery, target identification, and mechanistic studies, allowing researchers to characterize ligand binding properties, pinpoint critical binding sites, and refine the activity of lead compounds. The core principle......

• • AP-MS

  Affinity purification-mass spectrometry (AP-MS) integrates affinity purification techniques with mass spectrometry to investigate molecular interactions, the composition of protein complexes, and the dynamic behavior of functional biomolecules. This method leverages the specific binding properties of target molecules, such as proteins, nucleic acids, or small molecules, to isolate them from complex biological samples while simultaneously capturing associated interacting molecules. Subsequent identific......

• • Ligand Binding Assay

  The ligand binding assay is a fundamental methodology used to investigate interactions between biomolecules and ligands, providing critical insights into binding properties and kinetic parameters. Ligands, which may include small molecules, proteins, nucleic acids, or other chemical compounds, often play essential roles in biological processes such as enzymatic catalysis, signal transduction, and molecular transport. By assessing parameters such as binding affinity (e.g., dissociation constant), bindi......

• • Ligand Assay

  Ligand assay is a pivotal technique for investigating interactions between biomolecules and their ligands, enabling the determination of critical parameters such as binding mechanisms, kinetic profiles, and affinity. Ligands, which include small molecules, proteins, nucleic acids, or other chemical entities, bind specifically to target molecules. This binding often governs essential biological processes such as enzymatic catalysis, signal transduction, metabolic regulation, and gene expression. By qua......

• • Immunoprecipitation Analysis

  Immunoprecipitation analysis is a technique that utilizes the specific binding between antigens and antibodies to isolate and enrich target proteins or their complexes from complex biological samples. By capturing the target protein with a specific antibody and applying elution and detection methods, this technique facilitates the separation and functional analysis of proteins. It is a versatile and widely applied tool in molecular biology, proteomics, and biomedical research, particularly for explori......

• • Proximity Labeling

  Proximity Labeling is an innovative biological technique designed to investigate spatial protein interactions and molecular relationships within the cellular microenvironment. This approach leverages enzymes capable of catalyzing proximity-specific reactions, such as biotin ligase or peroxidase, to covalently attach distinct markers (e.g., biotin or fluorescent molecules) to molecules in the vicinity of target proteins. These labeled molecules are subsequently identified and quantified using mass spec......

• • Methods for Protein Structure Elucidation and Their Roles in Research

  Methods for protein structure elucidation and their roles in research are foundational to understanding the molecular mechanisms of biological processes. Techniques such as X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and cryo-electron microscopy (Cryo-EM) have enabled researchers to investigate protein architectures with unprecedented precision. These methodologies provide detailed insights into protein function and dynamics, driving progress in fields such as drug discovery,......

• • Protein Structure Analysis Methods and Applications

  Protein structure analysis methods and applications play a pivotal role in understanding biological functions and designing biomedical solutions. This article explores key methods and their advantages, limitations, and broader applications in research and industry.   X-ray Crystallography The three-dimensional atomic structure of a protein is determined by analyzing the diffraction patterns generated when X-rays interact with protein crystals.   1. Advantages Provides high-resolution structural data, ......

• • Protein Structure Determination Techniques and Applications

  Protein structure determination techniques and applications are foundational to elucidating the molecular mechanisms of life. These methodologies, encompassing X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and cryo-electron microscopy (Cryo-EM), have profoundly impacted research in biomedicine and pharmaceutical innovation. By leveraging these advanced techniques, researchers can uncover intricate protein architectures essential for biological function and therapeutic intervent......