Resources
Proteomics Databases
Metabolomics Databases
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• HCP Analysis by Mass Spectrometry
In the biopharmaceutical industry, host cell proteins (HCPs) refer to residual contaminant proteins derived from host cells during the production process. These proteins can co-purify with therapeutic products, posing potential risks to the safety and efficacy of the final product. Therefore, rigorous detection and control of HCPs are essential. HCP analysis by mass spectrometry is a highly sensitive and reliable approach for identifying and quantifying these contaminants, ensuring product purity and ......
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• Advances in HPLC Protein Analysis
HPLC protein analysis is a key technique in proteomics research, widely employed for protein separation and characterization. The method relies on using a liquid mobile phase under high pressure to flow through a chromatographic column containing a stationary phase, enabling the separation of components in a protein mixture. Given the complexity and diversity of proteins, HPLC protein analysis plays an essential role in evaluating protein purity, identifying protein modifications, quantifying protein ......
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Proteomics sequencing is an advanced analytical technology designed to comprehensively study the entire protein complement of an organism, playing a central role in understanding biological processes. Proteins function as molecular machines, executing essential tasks such as maintaining cellular structures, catalyzing biochemical reactions, and regulating physiological processes. Following the completion of the Human Genome Project, scientists recognized that decoding the genome-wide expression of gen......
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ABPP proteomics, short for activity-based protein profiling, is an advanced chemical probe-based technology designed to investigate protein functional states and activities. It enables monitoring and regulation of protein activity by employing specialized chemical probes that form stable covalent bonds with active sites in proteins. This approach is widely applied in modern biomedical research, particularly in drug discovery, disease mechanism elucidation, and biomarker identification. At the heart of......
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LC-MS proteomics is an analytical technique that integrates liquid chromatography (LC) and mass spectrometry (MS) to comprehensively study proteins in complex biological samples. At its core, this technology harnesses the separation efficiency of LC and the identification precision of MS, enabling scientists to perform protein identification, quantification, and post-translational modification analyses. LC-MS proteomics is indispensable in proteomic research, offering insights into intracellular prote......
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• Identification of Drug Targets
The identification of drug targets involves determining biomacromolecules, such as proteins, nucleic acids, and carbohydrates, that interact with drug molecules using various experimental and computational techniques. These targets are typically molecules associated with disease processes, playing critical roles in disease onset, progression, and development. As a cornerstone of drug discovery, target identification enhances our understanding of disease mechanisms, enables the identification of key mo......
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Isoform analysis refers to the identification, characterization, and quantification of compounds-known as isoforms-that share the same molecular formula but differ in their structures or spatial arrangements. In chemical and biological research, isoforms refer to molecules with identical chemical formulas but distinct molecular structures or stereochemical configurations. These structural differences often lead to significant variations in physicochemical properties, biological activities, metabolism,......
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The complete amino acid profile provides a detailed qualitative and quantitative analysis of both essential and non-essential amino acids, offering critical insights into protein composition and function. This analysis plays an essential role in fields such as protein research, biomedicine, food science, and agriculture. Amino acids, as the fundamental building blocks of proteins, are vital for numerous biological processes. Analyzing the complete amino acid profile allows researchers to understand pr......
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The protein pulldown assay is a widely employed in vitro technique for analyzing protein-protein interactions. By using a tagged bait protein to capture interacting partners, this method provides critical insights into protein relationships, binding affinity, and interaction specificity. Compared to yeast two-hybrid and co-immunoprecipitation assays, the protein pulldown assay offers direct and specific detection of interactions in a controlled environment. Its simplicity, high sensitivity, and abilit......
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Enzyme analysis involves investigating enzyme activity, kinetics, catalytic mechanisms, and regulatory effects (inhibition or activation) using a variety of experimental and technical approaches. Enzymes, which are protein or RNA molecules, act as biological catalysts, facilitating chemical reactions both intracellularly and extracellularly. They are often referred to as the "catalysts of life." The primary objective of enzyme analysis is to comprehensively understand enzymatic catalytic properties, r......
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