Resources

Proteomics Databases



Metabolomics Databases

• • LC-MS/MS Protein Sequencing in Proteomics

  LC-MS/MS protein sequencing is a powerful analytical technique for identifying and quantifying proteins in biological samples. By integrating the high-resolution separation capabilities of liquid chromatography (LC) with the sensitivity and specificity of tandem mass spectrometry (MS/MS), this method enables efficient separation, precise identification, and accurate quantification of proteins in complex biological mixtures. LC separates complex protein samples into smaller components, facilitating sub......

• • N and C Terminal Amino Acid Determination

  N and C terminal amino acid determination refers to the analytical process of identifying and characterizing the specific amino acid sequences at the N-terminal (amino terminus) and C-terminal (carboxyl terminus) of proteins using biochemical and mass spectrometry techniques. The N-terminal is the first amino acid in a polypeptide chain, while the C-terminal represents the last. By determining the sequences at these terminals, researchers can uncover critical information about protein function, evolut......

• • LC-MS/MS Analysis of Proteins

  LC-MS/MS analysis of proteins is a widely applied analytical technique in proteomics, integrating the high-resolution separation capability of liquid chromatography (LC) with the high sensitivity and specificity of tandem mass spectrometry (MS/MS). This approach enables comprehensive and detailed analysis of proteins in complex biological samples. The key advantage of LC-MS/MS analysis of proteins lies in its ability to simultaneously identify and quantify thousands of proteins in complex mixtures, pr......

• • Edman Degradation Method of Protein Sequencing

  The Edman degradation method of protein sequencing is a chemical approach that sequentially removes and identifies N-terminal amino acids from a polypeptide chain, enabling the precise determination of protein sequences. Renowned for its exceptional accuracy and reliability, this method is a pivotal tool in studying the structural and functional relationships of proteins. Its applications span various fields, including fundamental biological research, drug discovery, and disease mechanism exploration.......

• • Edman Sequencing Method

  The Edman sequencing method is a classical biochemical technique developed by Pehr Edman in the 1950s for determining the amino acid sequence of proteins. This method employs a stepwise chemical process to sequentially cleave amino acids from the N-terminus of a polypeptide chain. Each amino acid is subsequently separated and identified using chromatographic techniques, enabling the reconstruction of the protein's sequence. The core strength of the Edman sequencing method lies in its precise step-by-s......

• • LC-MS/MS Protein Quantification

  LC-MS/MS protein quantification combines the high-resolution separation capabilities of liquid chromatography (LC) with the sensitivity and precision of tandem mass spectrometry (MS/MS). This technique enables the comprehensive separation, identification, and quantification of complex protein mixtures, providing insights into protein expression levels, post-translational modifications, and interactions in biological systems.   Widely used across biomedical research, drug development, disease diagnosti......

• • LC-MS/MS Peptide Sequencing

  LC-MS/MS peptide sequencing is a powerful analytical method for identifying and analyzing peptide sequences in biological samples. By combining liquid chromatography (LC) for peptide separation with tandem mass spectrometry (MS/MS) for detailed sequence analysis, this technique provides a robust platform for studying complex proteomes. In LC, peptides are separated based on their retention times, while MS/MS generates peptide fragmentation spectra that allow the determination of their amino acid seque......

• • Mass Spec Peptide Sequencing

  Mass spec peptide sequencing is a highly sensitive and precise technique for analyzing proteins by sequencing their peptides to determine amino acid sequences. This method, a cornerstone of proteomics research, is particularly valuable for identifying and characterizing protein components in complex biological samples. It works by enzymatically or chemically breaking down proteins into smaller peptides, which are then analyzed for their mass using advanced mass spectrometry instruments.   Widely regar......

• • Mass Spectrometry Antibody Characterization

  Mass spectrometry antibody characterization is a powerful analytical method used to investigate the structure, composition, and modifications of antibodies at the molecular level. Antibodies, as essential components of the immune system, possess complex structures and diverse functions. This technique provides detailed insights into amino acid sequences, glycosylation patterns, and other post-translational modifications (PTMs), enabling a deeper understanding of antibody functionality and mechanisms. ......

• • Mass Spectrometry Amino Acid Sequencing

  Mass spectrometry amino acid sequencing is a highly sensitive and precise analytical method for determining the amino acid sequences of proteins and peptides. By leveraging the high resolution and sensitivity of mass spectrometry, this technique enables the detailed analysis of complex biological samples to elucidate protein structures. Amino acid sequences are the fundamental components of proteins, and understanding their arrangement is crucial for exploring protein functions, structures, and intera......