Resources
Proteomics Databases
Metabolomics Databases
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2D-DIGE proteomics, or two-dimensional difference gel electrophoresis proteomics, is a high-resolution protein separation technique derived from traditional two-dimensional gel electrophoresis (2-DE). By combining fluorescence dye labeling, it allows for the simultaneous analysis of multiple samples. In 2D-DIGE, protein mixtures are separated via isoelectric focusing and SDS-PAGE, enabling both resolution and quantification of samples on the same gel. This technique uniquely labels samples with differ......
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Antibiotic identification refers to the scientific techniques used to detect and confirm the types and characteristics of antibiotics. Antibiotics are secondary metabolites produced by microorganisms (such as bacteria and fungi) that can inhibit or kill other microorganisms. They are extensively used in both medical and agricultural applications. Antibiotics encompass a wide range of compounds, including penicillins, cephalosporins, aminoglycosides, and tetracyclines, each with distinct mechanisms of ......
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• SDS-PAGE Analysis of Proteins
SDS-PAGE analysis of proteins is a widely employed technique in biochemical and molecular biology research, designed for separating and analyzing individual proteins within complex mixtures. SDS-PAGE, which stands for Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, leverages the denaturing properties of SDS (Sodium Dodecyl Sulfate) and the resolving power of polyacrylamide gels to separate proteins based on their molecular weight. SDS, an anionic detergent, binds uniformly to proteins, mask......
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SDS-PAGE protein separation is a widely utilized electrophoretic technique in proteomics, enabling the separation of proteins based on their molecular weight. SDS, or Sodium Dodecyl Sulfate, is an anionic detergent that denatures proteins by disrupting their secondary and tertiary structures, ensuring uniform linear migration during electrophoresis. PAGE, short for Polyacrylamide Gel Electrophoresis, employs a gel matrix to facilitate size-based separation. In this method, protein samples are treated ......
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• Proximity Labeling Proteomics
Proximity labeling proteomics is an advanced biochemical approach designed to map protein spatial relationships and dynamic interactions within cells. By genetically fusing specific enzymes such as biotin ligase BirA or peroxidase APEX to target proteins, these enzymes can selectively label proximal proteins with biotin tags. The biotinylated proteins are subsequently enriched via streptavidin affinity purification and analyzed using high-resolution mass spectrometry for identification and quantificat......
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mRNA analysis is a method for investigating messenger RNA (mRNA) molecules, which serve as essential intermediaries in gene expression by conveying genetic instructions from DNA to the protein synthesis machinery. This technique primarily aims to determine gene expression levels and patterns, providing insights into the regulation of genes under various conditions. By examining mRNA expression, researchers can pinpoint disease-related genes, identify critical regulatory nodes in biological pathways, a......
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• Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry
Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful analytical technique extensively used in proteomics, chemistry, drug development, and clinical diagnostics. Its principle relies on the absorption of laser energy by matrix molecules, enabling the desorption and ionization of sample molecules. The flight time of these ions is then measured by a time-of-flight mass spectrometer to determine their mass. The efficiency of this technique largely depen......
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Enzyme Identification refers to the identification and characterization of enzymes in biological samples through a series of experimental and analytical approaches. Enzymes are proteins that serve as catalysts for biochemical reactions, playing critical roles in cellular metabolism, signal transduction, and gene expression regulation. Understanding enzyme types and functions is essential for elucidating biological mechanisms, developing novel therapeutics, and advancing biotechnological applications. ......
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Protein domain analysis is an essential component of protein research. Protein domains are distinct structural and functional units within protein macromolecules, capable of existing in a stable state and performing specific functions independently. In the drug development field, protein domain analysis is widely applied in the screening and design of drug targets. By analyzing protein domains, researchers can elucidate the binding mechanisms between drug molecules and protein targets, facilitating th......
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Protein expression analysis is an analytical technique used to investigate the levels and dynamic changes of proteins within organisms. By examining the expression levels and states of proteins in cells or tissues, this technique provides insights into their functional roles in biological processes. Through protein expression analysis, researchers can elucidate the molecular mechanisms underlying various biological phenomena, such as cell differentiation, signal transduction, and immune responses. In ......
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