Resources
Proteomics Databases
Metabolomics Databases
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Single cell mRNA sequencing is a revolutionary gene expression analysis technology that profiles mRNA expression at the single-cell level, enabling the exploration of cellular heterogeneity and dynamic changes under various biological states. This technique provides transformative insights into cell function, developmental processes, disease mechanisms, and intercellular communication, with wide-ranging applications in tumor research, immunology, neuroscience, and stem cell studies. Tumor tissues, com......
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2D gel analysis is a high-resolution method for separating and identifying proteins, widely utilized in proteomics research. This technique integrates two electrophoresis methods-soelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-to separate protein samples on a two-dimensional plane. In the first dimension, proteins are separated based on their isoelectric points (pI) through IEF. In the second dimension, SDS-PAGE resolves proteins according to their m......
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• PPI Protein-Protein Interaction
PPI Protein-Protein Interaction refers to the stable or transient associations between two or more protein molecules mediated by specific binding sites and non-covalent forces, such as hydrogen bonds, electrostatic interactions, and hydrophobic effects. These interactions are fundamental to cellular functions, underpinning nearly all physiological and pathological processes, including signal transduction, gene regulation, cell cycle control, metabolic pathways, and immune responses. PPIs can be classi......
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• Protein-Protein Interaction Mass Spectrometry
Protein-protein interaction mass spectrometry is a robust technique for investigating protein interaction networks using advanced mass spectrometry approaches. Protein-protein interactions (PPIs) underpin critical cellular processes, including signal transduction, cell cycle regulation, gene expression, metabolic control, and immune responses. These interactions vary dynamically between physiological and pathological states, with aberrant PPIs closely linked to diseases such as cancer, neurodegenerati......
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• Protein Sequencing by Edman Degradation
Protein Sequencing by Edman Degradation is a well-established method for determining the amino acid sequences of proteins. Developed in 1950 by the Swedish biochemist Pehr Edman, this technique enables stepwise sequencing by cleaving and labeling the N-terminal amino acids of a protein. Specifically, phenyl isothiocyanate (PITC) reacts with the N-terminal amino acid to form a phenylthiohydantoin derivative, which is selectively cleaved under acidic conditions to release a cyclic compound. This derivat......
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Peptide characterization is a fundamental aspect of proteomics research, focusing on the comprehensive analysis of peptide structures, sequences, properties, and functions. Peptides, which are small molecular fragments typically composed of 2 to 50 amino acids linked by peptide bonds, are derived from protein degradation. Despite their simpler structures compared to intact proteins, peptides exhibit diverse and complex biological functions, including roles in signal transduction, immune regulation, an......
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HCP coverage analysis is a key technique for detecting and quantifying residual host cell proteins (HCPs) in biopharmaceutical products. During the production of therapeutic proteins, host cells such as E. coli, yeast, or mammalian cells are commonly used. However, these cells may release or secrete various non-target proteins, collectively known as HCPs, during production. The presence of HCPs can compromise drug safety and efficacy, necessitating accurate detection and analysis throughout biopharmac......
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• HPLC Method for Protein Analysis
HPLC method for protein analysis is a robust and versatile technique used to separate protein components in a sample by employing a liquid mobile phase that flows through a chromatography column. This approach facilitates the fractionation of proteins based on specific properties such as size, hydrophobicity, and charge. Compared to traditional analytical methods, HPLC method for protein analysis offers significant advantages, including faster analysis and improved detection precision and accuracy. Re......
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Aptamer-based proteomics involves the analysis of protein composition and abundance through the specific recognition and binding capabilities of nucleic acid aptamers. These aptamers, composed of DNA or RNA sequences, are identified using SELEX (Systematic Evolution of Ligands by EXponential enrichment)—an in vitro selection process that isolates nucleic acid sequences with high affinity and specificity for target proteins from a vast library. Compared to traditional antibody-based detection methods......
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• Edman Degradation Protein Sequencing
Edman degradation protein sequencing is a classical technique for protein sequence analysis that facilitates the precise determination of protein amino acid sequences through sequential removal of amino acid residues. Developed in the 1950s by Swedish chemist Pehr Edman, this method has become an indispensable tool in proteomics research. The core principle of Edman degradation involves the selective binding of specific chemical reagents to the amino terminus of a protein, followed by stepwise removal......
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